Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods

Detalhes bibliográficos
Autor(a) principal: Mamede, A. P.
Data de Publicação: 2018
Outros Autores: Vassalo, A. R., Cunha, E., Gonçalves, D., Parker, S. F., Carvalho, L. A. E. Batista de, Marques, M. P. M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/84317
https://doi.org/10.1039/C8RA05660A
Resumo: Two different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.
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spelling Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methodsTwo different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/84317http://hdl.handle.net/10316/84317https://doi.org/10.1039/C8RA05660AporMamede, A. P.Vassalo, A. R.Cunha, E.Gonçalves, D.Parker, S. F.Carvalho, L. A. E. Batista deMarques, M. P. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-08-20T11:09:29ZPortal AgregadorONG
dc.title.none.fl_str_mv Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
spellingShingle Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
Mamede, A. P.
title_short Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_full Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_fullStr Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_full_unstemmed Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
title_sort Biomaterials from human bone – probing organic fraction removal by chemical and enzymatic methods
author Mamede, A. P.
author_facet Mamede, A. P.
Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Carvalho, L. A. E. Batista de
Marques, M. P. M.
author_role author
author2 Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Carvalho, L. A. E. Batista de
Marques, M. P. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mamede, A. P.
Vassalo, A. R.
Cunha, E.
Gonçalves, D.
Parker, S. F.
Carvalho, L. A. E. Batista de
Marques, M. P. M.
description Two different deproteination and defatting processes of human bone were investigated, by combined infrared and neutron techniques: a previously reported hydrazine extraction and a newly developed multi-enzymatic treatment. Complementary Fourier transform infrared total attenuated reflectance and inelastic neutron scattering spectroscopies were applied, allowing access to all vibrational modes of the samples. The effectiveness of the different experimental protocols for removing the organic constituents of bone (lipids and protein) was probed, as well as their effect on bone's structural and crystallinity features. The results thus gathered are expected to have an impact on bioanthropological, archaeological and medical sciences, namely regarding the development of novel biocompatible materials for orthopaedic xenografts.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/84317
http://hdl.handle.net/10316/84317
https://doi.org/10.1039/C8RA05660A
url http://hdl.handle.net/10316/84317
https://doi.org/10.1039/C8RA05660A
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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