Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/30864 https://doi.org/Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores”. Catarina Albuquerque, Sandra Cavaco, Pedro Caetano, Sandra Branco, Helena Monteiro, Marcos Ramos, Anabel Usié Chimenos, Célia Leão, Ana Botelho. Veterinary Microbiology Available online 10 January 2022, 109339. Doi:https://doi.org/10.1016/j.vetmic.2022.109339 https://doi.org/10.1016/j.vetmic.2022.109339 |
Resumo: | The Mediterranean climate region of Alentejo in the Southern of Portugal is an important sheep production centre but little is known about the presence and characteristics of Dichelobacter nodosus in association with Fusobacterium necrophorum in the different footrot lesion scores. DNA from 261 interdigital biopsy samples, taken from 14 footrot affected flocks and from three non-affected flocks, were analysed for the presence of D. nodosus and F. necrophorum by real-time PCR. Both virulence and serogroup were determined for 132 and 53 D. nodosus positive biopsy samples, respectively. The co-infection with both bacteria was the commonest epidemiological finding associated with a greater disease severity. There was a statistically significant association (p = 0.002) between footrot-affected flocks and the presence of D. nodosus. Most D. nodosus positive samples were virulent (96.2%) and belonged to serogroup B (90%). |
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Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores.ovine footrotinterdigital dermatitisDichelobacter nodosususobacterium necrophorummerino breedThe Mediterranean climate region of Alentejo in the Southern of Portugal is an important sheep production centre but little is known about the presence and characteristics of Dichelobacter nodosus in association with Fusobacterium necrophorum in the different footrot lesion scores. DNA from 261 interdigital biopsy samples, taken from 14 footrot affected flocks and from three non-affected flocks, were analysed for the presence of D. nodosus and F. necrophorum by real-time PCR. Both virulence and serogroup were determined for 132 and 53 D. nodosus positive biopsy samples, respectively. The co-infection with both bacteria was the commonest epidemiological finding associated with a greater disease severity. There was a statistically significant association (p = 0.002) between footrot-affected flocks and the presence of D. nodosus. Most D. nodosus positive samples were virulent (96.2%) and belonged to serogroup B (90%).Veterinary Microbiology2022-01-31T11:57:33Z2022-01-312022-01-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/30864https://doi.org/Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores”. Catarina Albuquerque, Sandra Cavaco, Pedro Caetano, Sandra Branco, Helena Monteiro, Marcos Ramos, Anabel Usié Chimenos, Célia Leão, Ana Botelho. Veterinary Microbiology Available online 10 January 2022, 109339. Doi:https://doi.org/10.1016/j.vetmic.2022.109339https://doi.org/10.1016/j.vetmic.2022.109339http://hdl.handle.net/10174/30864porndndndsmbb@uevora.ptndndndndndAlbuquerque, CatarinaCavaco, SandraCaetano, PedroBranco, SandraMonteiro, HelenaRamos, MarcosUsié Chimenos, AnabelLeão, CéliaBotelho, Anainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:29:52Zoai:dspace.uevora.pt:10174/30864Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:20:10.417294Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
title |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
spellingShingle |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. Albuquerque, Catarina ovine footrot interdigital dermatitis Dichelobacter nodosus usobacterium necrophorum merino breed |
title_short |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
title_full |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
title_fullStr |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
title_full_unstemmed |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
title_sort |
Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores. |
author |
Albuquerque, Catarina |
author_facet |
Albuquerque, Catarina Cavaco, Sandra Caetano, Pedro Branco, Sandra Monteiro, Helena Ramos, Marcos Usié Chimenos, Anabel Leão, Célia Botelho, Ana |
author_role |
author |
author2 |
Cavaco, Sandra Caetano, Pedro Branco, Sandra Monteiro, Helena Ramos, Marcos Usié Chimenos, Anabel Leão, Célia Botelho, Ana |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Albuquerque, Catarina Cavaco, Sandra Caetano, Pedro Branco, Sandra Monteiro, Helena Ramos, Marcos Usié Chimenos, Anabel Leão, Célia Botelho, Ana |
dc.subject.por.fl_str_mv |
ovine footrot interdigital dermatitis Dichelobacter nodosus usobacterium necrophorum merino breed |
topic |
ovine footrot interdigital dermatitis Dichelobacter nodosus usobacterium necrophorum merino breed |
description |
The Mediterranean climate region of Alentejo in the Southern of Portugal is an important sheep production centre but little is known about the presence and characteristics of Dichelobacter nodosus in association with Fusobacterium necrophorum in the different footrot lesion scores. DNA from 261 interdigital biopsy samples, taken from 14 footrot affected flocks and from three non-affected flocks, were analysed for the presence of D. nodosus and F. necrophorum by real-time PCR. Both virulence and serogroup were determined for 132 and 53 D. nodosus positive biopsy samples, respectively. The co-infection with both bacteria was the commonest epidemiological finding associated with a greater disease severity. There was a statistically significant association (p = 0.002) between footrot-affected flocks and the presence of D. nodosus. Most D. nodosus positive samples were virulent (96.2%) and belonged to serogroup B (90%). |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-31T11:57:33Z 2022-01-31 2022-01-10T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/30864 https://doi.org/Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores”. Catarina Albuquerque, Sandra Cavaco, Pedro Caetano, Sandra Branco, Helena Monteiro, Marcos Ramos, Anabel Usié Chimenos, Célia Leão, Ana Botelho. Veterinary Microbiology Available online 10 January 2022, 109339. Doi:https://doi.org/10.1016/j.vetmic.2022.109339 https://doi.org/10.1016/j.vetmic.2022.109339 http://hdl.handle.net/10174/30864 |
url |
http://hdl.handle.net/10174/30864 https://doi.org/Ovine footrot in Southern Portugal: detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores”. Catarina Albuquerque, Sandra Cavaco, Pedro Caetano, Sandra Branco, Helena Monteiro, Marcos Ramos, Anabel Usié Chimenos, Célia Leão, Ana Botelho. Veterinary Microbiology Available online 10 January 2022, 109339. Doi:https://doi.org/10.1016/j.vetmic.2022.109339 https://doi.org/10.1016/j.vetmic.2022.109339 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
nd nd nd smbb@uevora.pt nd nd nd nd nd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Veterinary Microbiology |
publisher.none.fl_str_mv |
Veterinary Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136683300487168 |