Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/21131 https://doi.org/10.1016/j.jelechem.2010.12.033 |
Resumo: | Biosensors have been prepared by modification of glassy carbon electrodes with functionalised multiwalled carbon nanotubes (MWCNT) dispersed in the room temperature ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethane)sulfonimide (BmimNTF2) and with lipase cross-linked with glutaraldehyde. The biosensor was applied to the determination of olive oil triglycerides by cyclic voltammetry. A phosphate buffer (pH 7.0) / BmimNO3 mixture is a better electrolyte than aqueous buffer alone. The response signal in the buffer-BmimNO3 mixture was found to increase with the number of cycles until a constant current was achieved. The calibration curve obtained exhibited a sigmoid-shape and a fourparameter model was used to fit the data which gave a limit of detection of 0.11 μg mL−1. Close inspection of such calibration curves showed two distinct linear regions indicating changes in the mechanism of the electrochemical response. Overall, the oxidative analytical response was found to be due to phenolic compounds present in the olive oil, released in the presence of lipase, rather than due to triglycerides per se. It was also found that there were no interferences from either cholesterol or glycerol. A possible mechanism of olive oil determination at a MWCNT-BmimNTF2/Lip biosensor is proposed. |
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Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble AnalytesRoom temperature ionic liquidsElectrochemical enzyme biosensorsLipaseGlutaraldehyde cross-linkingCarbon nanotubesTriglyceridesOlive oilBiosensors have been prepared by modification of glassy carbon electrodes with functionalised multiwalled carbon nanotubes (MWCNT) dispersed in the room temperature ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethane)sulfonimide (BmimNTF2) and with lipase cross-linked with glutaraldehyde. The biosensor was applied to the determination of olive oil triglycerides by cyclic voltammetry. A phosphate buffer (pH 7.0) / BmimNO3 mixture is a better electrolyte than aqueous buffer alone. The response signal in the buffer-BmimNO3 mixture was found to increase with the number of cycles until a constant current was achieved. The calibration curve obtained exhibited a sigmoid-shape and a fourparameter model was used to fit the data which gave a limit of detection of 0.11 μg mL−1. Close inspection of such calibration curves showed two distinct linear regions indicating changes in the mechanism of the electrochemical response. Overall, the oxidative analytical response was found to be due to phenolic compounds present in the olive oil, released in the presence of lipase, rather than due to triglycerides per se. It was also found that there were no interferences from either cholesterol or glycerol. A possible mechanism of olive oil determination at a MWCNT-BmimNTF2/Lip biosensor is proposed.Elsevier2011-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/21131http://hdl.handle.net/10316/21131https://doi.org/10.1016/j.jelechem.2010.12.033engPAULIUKAITE, Rasa [et al.] - Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes. "Journal of Electroanalytical Chemistry". ISSN 1572-6657. 656:1-2 (2011) 96-1011572-6657http://www.sciencedirect.com/science/journal/15726657Pauliukaite, RasaDoherty, Andrew P.Murnaghan, Kevin D.Brett, Christopher M. A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T13:17:39Zoai:estudogeral.uc.pt:10316/21131Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:48.352353Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
title |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
spellingShingle |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes Pauliukaite, Rasa Room temperature ionic liquids Electrochemical enzyme biosensors Lipase Glutaraldehyde cross-linking Carbon nanotubes Triglycerides Olive oil |
title_short |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
title_full |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
title_fullStr |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
title_full_unstemmed |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
title_sort |
Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes |
author |
Pauliukaite, Rasa |
author_facet |
Pauliukaite, Rasa Doherty, Andrew P. Murnaghan, Kevin D. Brett, Christopher M. A. |
author_role |
author |
author2 |
Doherty, Andrew P. Murnaghan, Kevin D. Brett, Christopher M. A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pauliukaite, Rasa Doherty, Andrew P. Murnaghan, Kevin D. Brett, Christopher M. A. |
dc.subject.por.fl_str_mv |
Room temperature ionic liquids Electrochemical enzyme biosensors Lipase Glutaraldehyde cross-linking Carbon nanotubes Triglycerides Olive oil |
topic |
Room temperature ionic liquids Electrochemical enzyme biosensors Lipase Glutaraldehyde cross-linking Carbon nanotubes Triglycerides Olive oil |
description |
Biosensors have been prepared by modification of glassy carbon electrodes with functionalised multiwalled carbon nanotubes (MWCNT) dispersed in the room temperature ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethane)sulfonimide (BmimNTF2) and with lipase cross-linked with glutaraldehyde. The biosensor was applied to the determination of olive oil triglycerides by cyclic voltammetry. A phosphate buffer (pH 7.0) / BmimNO3 mixture is a better electrolyte than aqueous buffer alone. The response signal in the buffer-BmimNO3 mixture was found to increase with the number of cycles until a constant current was achieved. The calibration curve obtained exhibited a sigmoid-shape and a fourparameter model was used to fit the data which gave a limit of detection of 0.11 μg mL−1. Close inspection of such calibration curves showed two distinct linear regions indicating changes in the mechanism of the electrochemical response. Overall, the oxidative analytical response was found to be due to phenolic compounds present in the olive oil, released in the presence of lipase, rather than due to triglycerides per se. It was also found that there were no interferences from either cholesterol or glycerol. A possible mechanism of olive oil determination at a MWCNT-BmimNTF2/Lip biosensor is proposed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/21131 http://hdl.handle.net/10316/21131 https://doi.org/10.1016/j.jelechem.2010.12.033 |
url |
http://hdl.handle.net/10316/21131 https://doi.org/10.1016/j.jelechem.2010.12.033 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PAULIUKAITE, Rasa [et al.] - Application of Room Temperature Ionic Liquids to the Development of Electrochemical Lipase Biosensing Systems for Water-Insoluble Analytes. "Journal of Electroanalytical Chemistry". ISSN 1572-6657. 656:1-2 (2011) 96-101 1572-6657 http://www.sciencedirect.com/science/journal/15726657 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133908776779776 |