Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70765 |
Resumo: | Pseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa. |
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Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa eliminationBio-based polymersDrug delivery platformNatural extractsTrigger-based releaseBactericidal effectsEngenharia e Tecnologia::Engenharia MédicaSaúde de qualidadePseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa.Authors acknowledge the Portuguese Foundation for Science and TechnologyMDPIUniversidade do MinhoMiranda, Catarina S.Antunes, Joana Isabel CostaHomem, Natália CândidoFelgueiras, Helena Prado20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70765eng2504-390010.3390/CGPM2020-07181https://www.mdpi.com/2504-3900/69/1/39info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22Zoai:repositorium.sdum.uminho.pt:1822/70765Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:21.775107Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
title |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
spellingShingle |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination Miranda, Catarina S. Bio-based polymers Drug delivery platform Natural extracts Trigger-based release Bactericidal effects Engenharia e Tecnologia::Engenharia Médica Saúde de qualidade |
title_short |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
title_full |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
title_fullStr |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
title_full_unstemmed |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
title_sort |
Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination |
author |
Miranda, Catarina S. |
author_facet |
Miranda, Catarina S. Antunes, Joana Isabel Costa Homem, Natália Cândido Felgueiras, Helena Prado |
author_role |
author |
author2 |
Antunes, Joana Isabel Costa Homem, Natália Cândido Felgueiras, Helena Prado |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Miranda, Catarina S. Antunes, Joana Isabel Costa Homem, Natália Cândido Felgueiras, Helena Prado |
dc.subject.por.fl_str_mv |
Bio-based polymers Drug delivery platform Natural extracts Trigger-based release Bactericidal effects Engenharia e Tecnologia::Engenharia Médica Saúde de qualidade |
topic |
Bio-based polymers Drug delivery platform Natural extracts Trigger-based release Bactericidal effects Engenharia e Tecnologia::Engenharia Médica Saúde de qualidade |
description |
Pseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70765 |
url |
http://hdl.handle.net/1822/70765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2504-3900 10.3390/CGPM2020-07181 https://www.mdpi.com/2504-3900/69/1/39 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132722591956992 |