Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination

Detalhes bibliográficos
Autor(a) principal: Miranda, Catarina S.
Data de Publicação: 2021
Outros Autores: Antunes, Joana Isabel Costa, Homem, Natália Cândido, Felgueiras, Helena Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/70765
Resumo: Pseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa.
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spelling Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa eliminationBio-based polymersDrug delivery platformNatural extractsTrigger-based releaseBactericidal effectsEngenharia e Tecnologia::Engenharia MédicaSaúde de qualidadePseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa.Authors acknowledge the Portuguese Foundation for Science and TechnologyMDPIUniversidade do MinhoMiranda, Catarina S.Antunes, Joana Isabel CostaHomem, Natália CândidoFelgueiras, Helena Prado20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70765eng2504-390010.3390/CGPM2020-07181https://www.mdpi.com/2504-3900/69/1/39info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:22Zoai:repositorium.sdum.uminho.pt:1822/70765Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:21.775107Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
title Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
spellingShingle Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
Miranda, Catarina S.
Bio-based polymers
Drug delivery platform
Natural extracts
Trigger-based release
Bactericidal effects
Engenharia e Tecnologia::Engenharia Médica
Saúde de qualidade
title_short Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
title_full Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
title_fullStr Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
title_full_unstemmed Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
title_sort Controlled release of cinnamon leaf oil from chitosan microcapsules embedded within a sodium alginate/gelatin hydrogel-like film for Pseudomonas aeruginosa elimination
author Miranda, Catarina S.
author_facet Miranda, Catarina S.
Antunes, Joana Isabel Costa
Homem, Natália Cândido
Felgueiras, Helena Prado
author_role author
author2 Antunes, Joana Isabel Costa
Homem, Natália Cândido
Felgueiras, Helena Prado
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Miranda, Catarina S.
Antunes, Joana Isabel Costa
Homem, Natália Cândido
Felgueiras, Helena Prado
dc.subject.por.fl_str_mv Bio-based polymers
Drug delivery platform
Natural extracts
Trigger-based release
Bactericidal effects
Engenharia e Tecnologia::Engenharia Médica
Saúde de qualidade
topic Bio-based polymers
Drug delivery platform
Natural extracts
Trigger-based release
Bactericidal effects
Engenharia e Tecnologia::Engenharia Médica
Saúde de qualidade
description Pseudomonas aeruginosa is considered a public threat, with antibiotics increasing their resistance. Essential oils (EOs) have demonstrated significant effects against microorganisms. However, due to their volatile nature, they cannot be used in their free-state. Here, hydrogel-like films were produced from a combination of sodium alginate (SA) and gelatin (GN) to serve as delivery platforms for the controlled release of cinnamon leaf oil (CLO) entrapped within chitosan (CS) microcapsules. The minimum inhibitory concentration (MIC) of CLO was established at 39.3 mg/mL against P. aeruginosa. CS microcapsules were prepared via ionotropic gelation with tripolyphosphate (TPP), encapsulating CLO at MIC. Successful production was confirmed by fluorescent microscopy using Nile red as a detection agent. Microcapsules were embedded within a biodegradable SA/GN polymeric matrix processed by solvent casting/phase inversion with SA/GN used at 70/30 polymer ratio at 2 wt.% SA concentration. A concentration of 2 wt.% CaCl2 was used as a coagulation bath. The CLO-containing CS microcapsules’ homogeneous distribution was guaranteed by successive vortex and blending processes applied prior to casting. CLO controlled release from the films was monitored in physiological pH for 24 h. Hydrated films were obtained, with the presence of loaded CS capsules being confirmed by FTIR. Qualitative/quantitative antimicrobial examinations validated the loaded film potential to fight P. aeruginosa.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/70765
url http://hdl.handle.net/1822/70765
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2504-3900
10.3390/CGPM2020-07181
https://www.mdpi.com/2504-3900/69/1/39
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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