Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos

Detalhes bibliográficos
Autor(a) principal: Teixeira, Ana
Data de Publicação: 2022
Outros Autores: Sárria, Marisa P., Pinto, Inês, Espiña, Begoña, Gomes, Andreia C, Dias, Alberto Carlos Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/80811
Resumo: The link between oxidative stress and environmental factors plays an important role in chronic degenerative diseases; therefore, exogenous antioxidants could be an effective alternative to combat disease progression and/or most significant symptoms. <i>Curcuma longa</i> L. (CL), commonly known as turmeric, is mostly composed of curcumin, a multivalent molecule described as having antioxidant, anti-inflammatory and neuroprotective properties. Poor chemical stability and low oral bioavailability and, consequently, poor absorption, rapid metabolism, and limited tissue distribution are major restrictions to its applicability. The advent of nanotechnology, by combining nanosacale with multi-functionality and bioavailability improvement, offers an opportunity to overcome these limitations. Therefore, in this work, poly-Ɛ-caprolactone (PCL) nanoparticles were developed to incorporate the methanolic extract of CL, and their bioactivity was assessed in comparison to free or encapsulated curcumin. Their toxicity was evaluated using zebrafish embryos by applying the Fish Embryo Acute Toxicity test, following recommended OECD guidelines. The protective effect against paraquat-induced oxidative damage of CL extract, free or encapsulated in PCL nanoparticles, was evaluated. This herbicide is known to cause oxidative damage and greatly affect neuromotor functions. The overall results indicate that CL-loaded PCL nanoparticles have an interesting protective capacity against paraquat-induced damage, particularly in neuromuscular development that goes well beyond that of CL extract itself and other known antioxidants.
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spelling Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryosCurcuminZebrafish embryogenesisOxidative stressNeuroprotectionPCL nanoparticlesParaquatScience & TechnologyThe link between oxidative stress and environmental factors plays an important role in chronic degenerative diseases; therefore, exogenous antioxidants could be an effective alternative to combat disease progression and/or most significant symptoms. <i>Curcuma longa</i> L. (CL), commonly known as turmeric, is mostly composed of curcumin, a multivalent molecule described as having antioxidant, anti-inflammatory and neuroprotective properties. Poor chemical stability and low oral bioavailability and, consequently, poor absorption, rapid metabolism, and limited tissue distribution are major restrictions to its applicability. The advent of nanotechnology, by combining nanosacale with multi-functionality and bioavailability improvement, offers an opportunity to overcome these limitations. Therefore, in this work, poly-Ɛ-caprolactone (PCL) nanoparticles were developed to incorporate the methanolic extract of CL, and their bioactivity was assessed in comparison to free or encapsulated curcumin. Their toxicity was evaluated using zebrafish embryos by applying the Fish Embryo Acute Toxicity test, following recommended OECD guidelines. The protective effect against paraquat-induced oxidative damage of CL extract, free or encapsulated in PCL nanoparticles, was evaluated. This herbicide is known to cause oxidative damage and greatly affect neuromotor functions. The overall results indicate that CL-loaded PCL nanoparticles have an interesting protective capacity against paraquat-induced damage, particularly in neuromuscular development that goes well beyond that of CL extract itself and other known antioxidants.Marisa P. Sarria was supported by Marie Skłodowska—Curie Actions from European Union’s 7th Framework Programme for Research, Technological Development and Demonstration under Grant Agreement 600375. This work was supported by the strategic programme UID/BIA/04050/ 2019 funded by national funds through the Fundação para a Ciência e a Tecnologia I.P. Marisa P. Sárria was supported by Marie Curie COFUND funding from the European Union’s 7th Framework Programme for research, technological development, and demonstration under grant agreement 600375. Begoña Espiña is supported by the co-funding from Northern Regional Operational Program through the Project NORTE-45-2015-02 Nanotechnology based functional solutions. B.E. acknowledges the financial support of the project SbDtoolBox—Nanotechnology-based tools and tests for Safer-by-Design nanomaterials, with the reference nº NORTE-01-0145-FEDER-000047, funded by Norte2020—North-Regional Operational Programme under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoTeixeira, AnaSárria, Marisa P.Pinto, InêsEspiña, BegoñaGomes, Andreia CDias, Alberto Carlos Pires2022-09-092022-09-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80811engTeixeira, A.; Sárria, M.P.; Pinto, I.; Espiña, B.; Gomes, A.C.; Dias, A.C.P. Protection against Paraquat-Induced Oxidative Stress by Curcuma longa Extract-Loaded Polymeric Nanoparticles in Zebrafish Embryos. Polymers 2022, 14, 3773. https://doi.org/10.3390/polym141837732073-436010.3390/polym141837733773https://www.mdpi.com/2073-4360/14/18/3773info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:18Zoai:repositorium.sdum.uminho.pt:1822/80811Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:24.143358Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
title Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
spellingShingle Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
Teixeira, Ana
Curcumin
Zebrafish embryogenesis
Oxidative stress
Neuroprotection
PCL nanoparticles
Paraquat
Science & Technology
title_short Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
title_full Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
title_fullStr Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
title_full_unstemmed Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
title_sort Protection against paraquat-induced oxidative stress by Curcuma longa extract-loaded polymeric nanoparticles in zebrafish embryos
author Teixeira, Ana
author_facet Teixeira, Ana
Sárria, Marisa P.
Pinto, Inês
Espiña, Begoña
Gomes, Andreia C
Dias, Alberto Carlos Pires
author_role author
author2 Sárria, Marisa P.
Pinto, Inês
Espiña, Begoña
Gomes, Andreia C
Dias, Alberto Carlos Pires
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Teixeira, Ana
Sárria, Marisa P.
Pinto, Inês
Espiña, Begoña
Gomes, Andreia C
Dias, Alberto Carlos Pires
dc.subject.por.fl_str_mv Curcumin
Zebrafish embryogenesis
Oxidative stress
Neuroprotection
PCL nanoparticles
Paraquat
Science & Technology
topic Curcumin
Zebrafish embryogenesis
Oxidative stress
Neuroprotection
PCL nanoparticles
Paraquat
Science & Technology
description The link between oxidative stress and environmental factors plays an important role in chronic degenerative diseases; therefore, exogenous antioxidants could be an effective alternative to combat disease progression and/or most significant symptoms. <i>Curcuma longa</i> L. (CL), commonly known as turmeric, is mostly composed of curcumin, a multivalent molecule described as having antioxidant, anti-inflammatory and neuroprotective properties. Poor chemical stability and low oral bioavailability and, consequently, poor absorption, rapid metabolism, and limited tissue distribution are major restrictions to its applicability. The advent of nanotechnology, by combining nanosacale with multi-functionality and bioavailability improvement, offers an opportunity to overcome these limitations. Therefore, in this work, poly-Ɛ-caprolactone (PCL) nanoparticles were developed to incorporate the methanolic extract of CL, and their bioactivity was assessed in comparison to free or encapsulated curcumin. Their toxicity was evaluated using zebrafish embryos by applying the Fish Embryo Acute Toxicity test, following recommended OECD guidelines. The protective effect against paraquat-induced oxidative damage of CL extract, free or encapsulated in PCL nanoparticles, was evaluated. This herbicide is known to cause oxidative damage and greatly affect neuromotor functions. The overall results indicate that CL-loaded PCL nanoparticles have an interesting protective capacity against paraquat-induced damage, particularly in neuromuscular development that goes well beyond that of CL extract itself and other known antioxidants.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-09
2022-09-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/80811
url https://hdl.handle.net/1822/80811
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Teixeira, A.; Sárria, M.P.; Pinto, I.; Espiña, B.; Gomes, A.C.; Dias, A.C.P. Protection against Paraquat-Induced Oxidative Stress by Curcuma longa Extract-Loaded Polymeric Nanoparticles in Zebrafish Embryos. Polymers 2022, 14, 3773. https://doi.org/10.3390/polym14183773
2073-4360
10.3390/polym14183773
3773
https://www.mdpi.com/2073-4360/14/18/3773
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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