The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo

Detalhes bibliográficos
Autor(a) principal: Duarte, Patrícia Rovisco e Silva
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/53864
Resumo: Tese de mestrado, Biologia Evolutiva e do Desenvolvimento, Universidade de Lisboa, Faculdade de Ciências, 2022
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spelling The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryoVangl2intercalação celularnó dorsalorientação da divisão celulardefeitos do tubo neuralTeses de mestrado - 2022Departamento de Biologia AnimalTese de mestrado, Biologia Evolutiva e do Desenvolvimento, Universidade de Lisboa, Faculdade de Ciências, 2022Mice carrying the homozygous Looptail (Lp) mutation, affecting the Vangl2 gene – a core element of the Wnt-PCP pathway – are known to develop craniorachischisis, a rare and severe neural tube defect. This derives from an impairment of convergent extensions cellular movements at the neural plate midline level, resulting in a broad floor plate and failure of the initiation of Closure 1 of the neural tube. Neurulation in an important developmental step that is strongly linked to node regression and the establishment of the notochord, both of which appear to be affected by the Lp mutation. Studies on the mouse embryonic node have focused mostly on its ventral component, as this is the one thought to contain all the properties of the organizer. Because the dorsal component of the node – or the dorsal midline primordium (DMP) – has been treated, over the years, as an extension of the epiblast, little is known about the cellular behaviour of this layer. Some evidence suggests that DMP cells may participate in notochordal formation, raising the question of whether or not the DMP contributes to cell intercalation at midline level and overall node regression. Following this hypothesis, and since (ventral) node morphology and midline formation have been shown to be affected in Vangl2Lp/Lp embryos, it is possible that the Lp mutation may also promote defects in the DMP. The main objective of the present work is to shed some light on how DMP cells behave during node regression, and how this process may affect neural plate midline establishment, later on in development. For this purpose, i) apical morphology and cells dynamics in the Vangl2+/+ DMP were characterized ii) and compared to that found in Vangl2Lp/Lp embryos; iii) the parameters found altered in the Vangl2Lp/Lp DMP were also assessed in the Vangl2+/Lp embryo; iv) possible changes at the apical level of the DMP, through its development, were assessed in different genotypes. Through whole mount immunohistochemistry and the use of an antibody against the zonula occludens 1 (ZO1) protein, it was possible to label the apical domain of DMP cells. This, coupled with image segmentation techniques, allowed a cell-by-cell approach which focused, solely, on this dorsal component of the embryonic node. Furthermore, the use of the Lp mouse strain helped to inquire how the Wnt/PCP pathway may act in the context of DMP development. The results reported here suggest an active contribution of the DMP for midline formation and that the Wnt/PCP pathway may be required for proper apical morphology within the DMP. Furthermore, the results suggest that a single copy of the Lp mutation may be sufficient to promote a defect in cell division orientation, and that this, coupled with a partial defect in apical constriction, may be a driver for the failure of Closure 1 in the NT of Vangl2Lp/Lp mice.Ybot-Gonzalez, PatriciaThorsteinsdóttir, Sólveig, 1962-Repositório da Universidade de LisboaDuarte, Patrícia Rovisco e Silva202220222025-02-24T00:00:00Z2022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/53864enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:00:05Zoai:repositorio.ul.pt:10451/53864Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:50.718926Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
title The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
spellingShingle The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
Duarte, Patrícia Rovisco e Silva
Vangl2
intercalação celular
nó dorsal
orientação da divisão celular
defeitos do tubo neural
Teses de mestrado - 2022
Departamento de Biologia Animal
title_short The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
title_full The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
title_fullStr The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
title_full_unstemmed The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
title_sort The Looptail mutation promotes a defect in apical morphology in the dorsal midline primordium of the early mouse embryo
author Duarte, Patrícia Rovisco e Silva
author_facet Duarte, Patrícia Rovisco e Silva
author_role author
dc.contributor.none.fl_str_mv Ybot-Gonzalez, Patricia
Thorsteinsdóttir, Sólveig, 1962-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Duarte, Patrícia Rovisco e Silva
dc.subject.por.fl_str_mv Vangl2
intercalação celular
nó dorsal
orientação da divisão celular
defeitos do tubo neural
Teses de mestrado - 2022
Departamento de Biologia Animal
topic Vangl2
intercalação celular
nó dorsal
orientação da divisão celular
defeitos do tubo neural
Teses de mestrado - 2022
Departamento de Biologia Animal
description Tese de mestrado, Biologia Evolutiva e do Desenvolvimento, Universidade de Lisboa, Faculdade de Ciências, 2022
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022-01-01T00:00:00Z
2025-02-24T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/53864
url http://hdl.handle.net/10451/53864
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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