The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration

Detalhes bibliográficos
Autor(a) principal: Uarrota, Virgílio Gavicho
Data de Publicação: 2016
Outros Autores: Moresco, Rodolfo, Schmidt, Eder Carlos, Bouzon, Zenilda Laurita, Nunes, Eduardo da Costa, Neubert, Enilto de Oliveira, Peruch, Luiz Augusto Martins, Rocha, Miguel, Maraschin, Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/38530
Resumo: Abstract This study aimed to investigate the role of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), polysaccharides, and protein contents associated with the early events of postharvest physiological deterioration (PPD) in cassava roots. Increases in APX and GPX activity, as well as total protein contents occurred from 3 to 5 days of storage and were correlated with the delay of PPD. Cassava samples stained with periodic acid-Schiff (PAS) highlighted the presence of starch and cellulose. Degradation of starch granules during PPD was also detected. Slight metachromatic reaction with toluidine blue is indicative of increasing of acidic polysaccharides and may play an important role in PPD delay. Principal component analysis (PCA) classified samples according to their levels of enzymatic activity based on the decision tree model which showed GPX and total protein amounts to be correlated with PPD. The Oriental (ORI) cultivar was more susceptible to PPD.
id RCAP_278a705973afc65b28f013c296613a87
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/38530
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deteriorationAntioxidant enzymesCassava rootsPostharvestL-ascorbic acid peroxidasePolysaccharidesPostharvestScience & TechnologyAbstract This study aimed to investigate the role of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), polysaccharides, and protein contents associated with the early events of postharvest physiological deterioration (PPD) in cassava roots. Increases in APX and GPX activity, as well as total protein contents occurred from 3 to 5 days of storage and were correlated with the delay of PPD. Cassava samples stained with periodic acid-Schiff (PAS) highlighted the presence of starch and cellulose. Degradation of starch granules during PPD was also detected. Slight metachromatic reaction with toluidine blue is indicative of increasing of acidic polysaccharides and may play an important role in PPD delay. Principal component analysis (PCA) classified samples according to their levels of enzymatic activity based on the decision tree model which showed GPX and total protein amounts to be correlated with PPD. The Oriental (ORI) cultivar was more susceptible to PPD.This work was supported by PEC-PG ("Programa de Estudantes Convenio de Pos-Graduacao") coordinated by CAPES ("Coorde nacao de Aperfeicoamento de Pessoal de Nivel Superior"). CNPq has provided a research fellowship to Marcelo Maraschin.Elsevier B.V.Universidade do MinhoUarrota, Virgílio GavichoMoresco, RodolfoSchmidt, Eder CarlosBouzon, Zenilda LauritaNunes, Eduardo da CostaNeubert, Enilto de OliveiraPeruch, Luiz Augusto MartinsRocha, MiguelMaraschin, Marcelo2016-04-152016-04-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/38530engUarrota, Virgílio Gavicho; Moresco, Rodolfo; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Nunes, Eduardo da Costa; Neubert, Enilto de Oliveira; Peruch, Luiz Augusto Martins; Rocha, Miguel; Maraschin, Marcelo, The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration. Food Chemistry, 197(Part A), 737-746, 20160308-81460308-814610.1016/j.foodchem.2015.11.02526617011http://www.elsevier.com/locate/issn/03088146info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:16Zoai:repositorium.sdum.uminho.pt:1822/38530Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:34:41.148511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
title The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
spellingShingle The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
Uarrota, Virgílio Gavicho
Antioxidant enzymes
Cassava roots
Postharvest
L-ascorbic acid peroxidase
Polysaccharides
Postharvest
Science & Technology
title_short The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
title_full The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
title_fullStr The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
title_full_unstemmed The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
title_sort The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
author Uarrota, Virgílio Gavicho
author_facet Uarrota, Virgílio Gavicho
Moresco, Rodolfo
Schmidt, Eder Carlos
Bouzon, Zenilda Laurita
Nunes, Eduardo da Costa
Neubert, Enilto de Oliveira
Peruch, Luiz Augusto Martins
Rocha, Miguel
Maraschin, Marcelo
author_role author
author2 Moresco, Rodolfo
Schmidt, Eder Carlos
Bouzon, Zenilda Laurita
Nunes, Eduardo da Costa
Neubert, Enilto de Oliveira
Peruch, Luiz Augusto Martins
Rocha, Miguel
Maraschin, Marcelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Uarrota, Virgílio Gavicho
Moresco, Rodolfo
Schmidt, Eder Carlos
Bouzon, Zenilda Laurita
Nunes, Eduardo da Costa
Neubert, Enilto de Oliveira
Peruch, Luiz Augusto Martins
Rocha, Miguel
Maraschin, Marcelo
dc.subject.por.fl_str_mv Antioxidant enzymes
Cassava roots
Postharvest
L-ascorbic acid peroxidase
Polysaccharides
Postharvest
Science & Technology
topic Antioxidant enzymes
Cassava roots
Postharvest
L-ascorbic acid peroxidase
Polysaccharides
Postharvest
Science & Technology
description Abstract This study aimed to investigate the role of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), polysaccharides, and protein contents associated with the early events of postharvest physiological deterioration (PPD) in cassava roots. Increases in APX and GPX activity, as well as total protein contents occurred from 3 to 5 days of storage and were correlated with the delay of PPD. Cassava samples stained with periodic acid-Schiff (PAS) highlighted the presence of starch and cellulose. Degradation of starch granules during PPD was also detected. Slight metachromatic reaction with toluidine blue is indicative of increasing of acidic polysaccharides and may play an important role in PPD delay. Principal component analysis (PCA) classified samples according to their levels of enzymatic activity based on the decision tree model which showed GPX and total protein amounts to be correlated with PPD. The Oriental (ORI) cultivar was more susceptible to PPD.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-15
2016-04-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/38530
url http://hdl.handle.net/1822/38530
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Uarrota, Virgílio Gavicho; Moresco, Rodolfo; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Nunes, Eduardo da Costa; Neubert, Enilto de Oliveira; Peruch, Luiz Augusto Martins; Rocha, Miguel; Maraschin, Marcelo, The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration. Food Chemistry, 197(Part A), 737-746, 2016
0308-8146
0308-8146
10.1016/j.foodchem.2015.11.025
26617011
http://www.elsevier.com/locate/issn/03088146
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132870065782784

Registros relacionados