Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress

Detalhes bibliográficos
Autor(a) principal: Lopes, Ana
Data de Publicação: 2023
Outros Autores: Azevedo-Silva, João, Carsanba, Erdem, Pintado, Manuela, Oliveira, Ana Sofia, Ferreira, Carlos, Pereira, Joana Odila, Carvalho, Ana P., Oliveira, Carla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/41086
Resumo: Yeast cells face various stress factors during industrial fermentations, since they are exposed to harsh environmental conditions, which may impair biomolecules productivity and yield. In this work, the use of an antioxidant peptide extract obtained from industrial spent yeast was explored as supplement for Saccharomyces cerevisiae fermentation to prevent a common bottleneck: oxidative stress. For that, a recombinant yeast strain, producer of β-farnesene, was firstly incubated with 0.5 and 0.7 g/L peptide extract, in the presence and absence of hydrogen peroxide (an oxidative stress inducer), for 1–5 h, and then assayed for intracellular reactive oxygen species, and growth ability in agar spot assays. Results showed that under 2 mM H2O2, the peptide extract could improve cells growth and reduce reactive oxygen species production. Therefore, this antioxidant effect was further evaluated in shake-flasks and 2-L bioreactor batch fermentations. Peptide extract (0.7 g/L) was able to increase yeast resistance to the oxidative stress promoted by 2 mM H2O2, by reducing reactive oxygen species levels between 1.2- and 1.7-fold in bioreactor and between 1.2- and 3-fold in shake-flask fermentations. Moreover, improvements on yeast cell density of up to 1.5-fold and 2-fold, and on biomolecule concentration of up to 1.6-fold and 2.8-fold, in bioreactor and shake-flasks, respectively, were obtained. Thus, culture medium supplementation with antioxidant peptide extracted from industrial spent yeast is a promising strategy to improve fermentation performance while valuing biomass waste. This valorization can promote a sustainable and eco-friendly solution for the biotechnology industry by the implementation of a circular economy model.
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spelling Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stressAntioxidant activityBioreactor fermentationCircular economyFarnesene productionPeptide-rich extractReactive oxygen speciesYeast cells face various stress factors during industrial fermentations, since they are exposed to harsh environmental conditions, which may impair biomolecules productivity and yield. In this work, the use of an antioxidant peptide extract obtained from industrial spent yeast was explored as supplement for Saccharomyces cerevisiae fermentation to prevent a common bottleneck: oxidative stress. For that, a recombinant yeast strain, producer of β-farnesene, was firstly incubated with 0.5 and 0.7 g/L peptide extract, in the presence and absence of hydrogen peroxide (an oxidative stress inducer), for 1–5 h, and then assayed for intracellular reactive oxygen species, and growth ability in agar spot assays. Results showed that under 2 mM H2O2, the peptide extract could improve cells growth and reduce reactive oxygen species production. Therefore, this antioxidant effect was further evaluated in shake-flasks and 2-L bioreactor batch fermentations. Peptide extract (0.7 g/L) was able to increase yeast resistance to the oxidative stress promoted by 2 mM H2O2, by reducing reactive oxygen species levels between 1.2- and 1.7-fold in bioreactor and between 1.2- and 3-fold in shake-flask fermentations. Moreover, improvements on yeast cell density of up to 1.5-fold and 2-fold, and on biomolecule concentration of up to 1.6-fold and 2.8-fold, in bioreactor and shake-flasks, respectively, were obtained. Thus, culture medium supplementation with antioxidant peptide extracted from industrial spent yeast is a promising strategy to improve fermentation performance while valuing biomass waste. This valorization can promote a sustainable and eco-friendly solution for the biotechnology industry by the implementation of a circular economy model.Veritati - Repositório Institucional da Universidade Católica PortuguesaLopes, AnaAzevedo-Silva, JoãoCarsanba, ErdemPintado, ManuelaOliveira, Ana SofiaFerreira, CarlosPereira, Joana OdilaCarvalho, Ana P.Oliveira, Carla2023-05-10T14:46:10Z2023-06-012023-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/41086eng0175-759810.1007/s00253-023-12514-38515321821737086282000973374700001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-18T01:38:39Zoai:repositorio.ucp.pt:10400.14/41086Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:33:45.870506Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
title Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
spellingShingle Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
Lopes, Ana
Antioxidant activity
Bioreactor fermentation
Circular economy
Farnesene production
Peptide-rich extract
Reactive oxygen species
title_short Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
title_full Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
title_fullStr Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
title_full_unstemmed Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
title_sort Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress
author Lopes, Ana
author_facet Lopes, Ana
Azevedo-Silva, João
Carsanba, Erdem
Pintado, Manuela
Oliveira, Ana Sofia
Ferreira, Carlos
Pereira, Joana Odila
Carvalho, Ana P.
Oliveira, Carla
author_role author
author2 Azevedo-Silva, João
Carsanba, Erdem
Pintado, Manuela
Oliveira, Ana Sofia
Ferreira, Carlos
Pereira, Joana Odila
Carvalho, Ana P.
Oliveira, Carla
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Lopes, Ana
Azevedo-Silva, João
Carsanba, Erdem
Pintado, Manuela
Oliveira, Ana Sofia
Ferreira, Carlos
Pereira, Joana Odila
Carvalho, Ana P.
Oliveira, Carla
dc.subject.por.fl_str_mv Antioxidant activity
Bioreactor fermentation
Circular economy
Farnesene production
Peptide-rich extract
Reactive oxygen species
topic Antioxidant activity
Bioreactor fermentation
Circular economy
Farnesene production
Peptide-rich extract
Reactive oxygen species
description Yeast cells face various stress factors during industrial fermentations, since they are exposed to harsh environmental conditions, which may impair biomolecules productivity and yield. In this work, the use of an antioxidant peptide extract obtained from industrial spent yeast was explored as supplement for Saccharomyces cerevisiae fermentation to prevent a common bottleneck: oxidative stress. For that, a recombinant yeast strain, producer of β-farnesene, was firstly incubated with 0.5 and 0.7 g/L peptide extract, in the presence and absence of hydrogen peroxide (an oxidative stress inducer), for 1–5 h, and then assayed for intracellular reactive oxygen species, and growth ability in agar spot assays. Results showed that under 2 mM H2O2, the peptide extract could improve cells growth and reduce reactive oxygen species production. Therefore, this antioxidant effect was further evaluated in shake-flasks and 2-L bioreactor batch fermentations. Peptide extract (0.7 g/L) was able to increase yeast resistance to the oxidative stress promoted by 2 mM H2O2, by reducing reactive oxygen species levels between 1.2- and 1.7-fold in bioreactor and between 1.2- and 3-fold in shake-flask fermentations. Moreover, improvements on yeast cell density of up to 1.5-fold and 2-fold, and on biomolecule concentration of up to 1.6-fold and 2.8-fold, in bioreactor and shake-flasks, respectively, were obtained. Thus, culture medium supplementation with antioxidant peptide extracted from industrial spent yeast is a promising strategy to improve fermentation performance while valuing biomass waste. This valorization can promote a sustainable and eco-friendly solution for the biotechnology industry by the implementation of a circular economy model.
publishDate 2023
dc.date.none.fl_str_mv 2023-05-10T14:46:10Z
2023-06-01
2023-06-01T00:00:00Z
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