Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation

Detalhes bibliográficos
Autor(a) principal: Balmayor, E. R.
Data de Publicação: 2015
Outros Autores: Baran, T. E., Unger, M., Marques, A. P., Azevedo, H. S., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/40668
Resumo: The effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.
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spelling Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulationcytotoxicityenzymatic degradationgentamicinmicroparticlesStarch-conjugated chitosanScience & TechnologyThe effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.Contract grant sponsor: Marie Curie Alea Jacta EST; contract grant number: MEST-CT-2004-008104 Contract grant sponsor: European NoE EXPERTISSUES; contract grant number: NMP3-CT-2004-500283WileyUniversidade do MinhoBalmayor, E. R.Baran, T. E.Unger, M.Marques, A. P.Azevedo, H. S.Reis, R. L.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/40668engBalmayor, E. R., Baran, T. E., Unger, M., Marques, A. P., Azevedo, H. S., & Reis, R. L. (2015). Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 103(8), 1610-1620. doi: 10.1002/jbm.b.333431552-498110.1002/jbm.b.3334325970852http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33343/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:24Zoai:repositorium.sdum.uminho.pt:1822/40668Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:00.521148Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
title Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
spellingShingle Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
Balmayor, E. R.
cytotoxicity
enzymatic degradation
gentamicin
microparticles
Starch-conjugated chitosan
Science & Technology
title_short Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
title_full Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
title_fullStr Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
title_full_unstemmed Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
title_sort Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation
author Balmayor, E. R.
author_facet Balmayor, E. R.
Baran, T. E.
Unger, M.
Marques, A. P.
Azevedo, H. S.
Reis, R. L.
author_role author
author2 Baran, T. E.
Unger, M.
Marques, A. P.
Azevedo, H. S.
Reis, R. L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Balmayor, E. R.
Baran, T. E.
Unger, M.
Marques, A. P.
Azevedo, H. S.
Reis, R. L.
dc.subject.por.fl_str_mv cytotoxicity
enzymatic degradation
gentamicin
microparticles
Starch-conjugated chitosan
Science & Technology
topic cytotoxicity
enzymatic degradation
gentamicin
microparticles
Starch-conjugated chitosan
Science & Technology
description The effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/40668
url https://hdl.handle.net/1822/40668
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Balmayor, E. R., Baran, T. E., Unger, M., Marques, A. P., Azevedo, H. S., & Reis, R. L. (2015). Presence of starch enhances in vitro biodegradation and biocompatibility of a gentamicin delivery formulation. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 103(8), 1610-1620. doi: 10.1002/jbm.b.33343
1552-4981
10.1002/jbm.b.33343
25970852
http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33343/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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