The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress

Detalhes bibliográficos
Autor(a) principal: Silva, Liliana
Data de Publicação: 2018
Outros Autores: Baptista, Joana, Bately, Charlotte, Andrews, Simon, Saraiva, Ligia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/59829
Resumo: The RIC (Repair of Iron Clusters) protein of Escherichia coli is a di-iron hemerythrin-like protein that has a proposed function in repairing stress-damaged iron-sulphur clusters. In this work, we performed a Bacterial Two Hybrid screening to search for RIC-protein interaction partners in E. coli. As a result, the DNA-binding protein from starved cells (Dps) was identified and its potential interaction with RIC was tested by BACTH, Bimolecular-Fluorescence-Complementation and pull-down assays. (...)
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spelling The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stressDpsE. coliYtfEdi-ironThe RIC (Repair of Iron Clusters) protein of Escherichia coli is a di-iron hemerythrin-like protein that has a proposed function in repairing stress-damaged iron-sulphur clusters. In this work, we performed a Bacterial Two Hybrid screening to search for RIC-protein interaction partners in E. coli. As a result, the DNA-binding protein from starved cells (Dps) was identified and its potential interaction with RIC was tested by BACTH, Bimolecular-Fluorescence-Complementation and pull-down assays. (...)American Society for MicrobiologyRUNSilva, LilianaBaptista, JoanaBately, CharlotteAndrews, SimonSaraiva, Ligia2019-02-07T18:03:42Z2018-122018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/59829engSilva LSO, Baptista JM, Batley C, Andrews SC, Saraiva LM. 2018. The di-iron RIC protein (YtfE) of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC protein-mediated redox stress. J Bacteriol 200:e00527-18. https://doi.org/10.1128/JB.00527-18.10.1128/JB.00527-18info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:37:07Zoai:run.unl.pt:10362/59829Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:37:07Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
title The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
spellingShingle The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
Silva, Liliana
Dps
E. coli
YtfE
di-iron
title_short The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
title_full The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
title_fullStr The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
title_full_unstemmed The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
title_sort The di-iron RIC (YtfE) protein 1 of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC-protein-mediated redox stress
author Silva, Liliana
author_facet Silva, Liliana
Baptista, Joana
Bately, Charlotte
Andrews, Simon
Saraiva, Ligia
author_role author
author2 Baptista, Joana
Bately, Charlotte
Andrews, Simon
Saraiva, Ligia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Silva, Liliana
Baptista, Joana
Bately, Charlotte
Andrews, Simon
Saraiva, Ligia
dc.subject.por.fl_str_mv Dps
E. coli
YtfE
di-iron
topic Dps
E. coli
YtfE
di-iron
description The RIC (Repair of Iron Clusters) protein of Escherichia coli is a di-iron hemerythrin-like protein that has a proposed function in repairing stress-damaged iron-sulphur clusters. In this work, we performed a Bacterial Two Hybrid screening to search for RIC-protein interaction partners in E. coli. As a result, the DNA-binding protein from starved cells (Dps) was identified and its potential interaction with RIC was tested by BACTH, Bimolecular-Fluorescence-Complementation and pull-down assays. (...)
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2018-12-01T00:00:00Z
2019-02-07T18:03:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/59829
url http://hdl.handle.net/10362/59829
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva LSO, Baptista JM, Batley C, Andrews SC, Saraiva LM. 2018. The di-iron RIC protein (YtfE) of Escherichia coli interacts with the DNA-binding protein from starved cells (Dps) to diminish RIC protein-mediated redox stress. J Bacteriol 200:e00527-18. https://doi.org/10.1128/JB.00527-18.
10.1128/JB.00527-18
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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