Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

Detalhes bibliográficos
Autor(a) principal: Silva, Inês Vieira
Data de Publicação: 2021
Outros Autores: Soares, Bárbara P., Pimpão, Catarina, Pinto, Rui M.A., Costa, Teresa, Freire, João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/21343
Resumo: Research Article
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spelling Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of pigletspigletsdietsaquaporinscystineResearch ArticleThe regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulationPlos OneRepositório da Universidade de LisboaSilva, Inês VieiraSoares, Bárbara P.Pimpão, CatarinaPinto, Rui M.A.Costa, TeresaFreire, João2021-05-25T14:25:40Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21343engVieira da Silva I, P. Soares B, Pimpão C, M. A. Pinto R, Costa T, Freire JPB, et al. (2021) Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PLoS ONE 16(1): e0245739https://doi. org/10.1371/journal.pone.0245739info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:50:47Zoai:www.repository.utl.pt:10400.5/21343Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:05:56.824190Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
spellingShingle Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
Silva, Inês Vieira
piglets
diets
aquaporins
cystine
title_short Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_fullStr Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_full_unstemmed Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
title_sort Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
author Silva, Inês Vieira
author_facet Silva, Inês Vieira
Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M.A.
Costa, Teresa
Freire, João
author_role author
author2 Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M.A.
Costa, Teresa
Freire, João
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Inês Vieira
Soares, Bárbara P.
Pimpão, Catarina
Pinto, Rui M.A.
Costa, Teresa
Freire, João
dc.subject.por.fl_str_mv piglets
diets
aquaporins
cystine
topic piglets
diets
aquaporins
cystine
description Research Article
publishDate 2021
dc.date.none.fl_str_mv 2021-05-25T14:25:40Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21343
url http://hdl.handle.net/10400.5/21343
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vieira da Silva I, P. Soares B, Pimpão C, M. A. Pinto R, Costa T, Freire JPB, et al. (2021) Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PLoS ONE 16(1): e0245739
https://doi. org/10.1371/journal.pone.0245739
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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