Development of wound healing biomaterials based on bacterial exopolysaccharides
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/129170 |
Resumo: | Currently, biopolymers have aroused great interest mainly in the food, pharmaceutical, cosmetic, and also in the biomedical fields, due to the intelligent and versatile properties that present, namely, biocompatibility, biodegradability, and non-toxicity. In addition to these properties, many polymers have vital characteristics that assist in wound healing processes and, when incorporated into nanoparticles of elements such as silver (Ag), gold (Au), and selenium (Se), further increase the effectiveness of these polymers. Therefore, the main objective of this study was to determine the best conditions for the preparation of biocomposites for application in the treatment of wounds, using different extracellular bacterial polysaccharides (EPSs) (EPS1, EPS2, EPS3, EPS4, and FucoPol) as reducing and stabilizing agents for the synthesis of silver nanoparticles (AgNP), gold (AuNP) and selenium (SeNP). EPSs were characterized as to the composition in sugars and substituting groups, because, depending on the composition, they can interfere in some way in the properties of each one. EPS1 is composed mainly of mannose (56.1% mol), EPS2 and EPS3 are composed mainly of glucose, 67% mol and 39.1% mol, respectively, EPS4 is composed mainly of glucuronic acid (55.9% mol) and FucoPol by fucose (34.9 mol%). Biocomposites were also characterized by UV-vis spectroscopy, FTIR, TGA, XRD, DLS, Zeta potential and ICP. Cytotoxicity and wound healing effect of EPSs and EPS/NPs biocomposites were analyzed in human keratinocyte cells (HaCaT cell line). The samples were tested using a polymer concentration of 62.5 - 1000 mg/L and none of the samples showed cytotoxicity, except for the EPS3/Ag+ biocomposite when tested at a polymer concentration above 125 mg/L, with a content Ag+ above 6.9 mg/L (decreased cell viability by ~91.9%). On the other hand, among all EPSs and their functionalized materials, FucoPol and the biocomposite FucoPol/AgNP exhibited a greater capacity to promote keratinocyte migration in vitro. Although EPS1 did not present wound healing effect, the biocomposites EPS1/AgNP (100 mg/L) and EPS1/AuNP (500 mg/L) showed to promote the migration of HaCaT cells. The results obtained suggest that both FucoPol and the biocomposite FucoPol/AgNP, as well as the biocomposites EPS1/AgNP and EPS1/AuNP, may have potential application in dermo-cosmetics, mainly in wound healing mechanisms. |
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Development of wound healing biomaterials based on bacterial exopolysaccharidesExopolysaccharidesnanoparticlescytotoxicitywound healingbiopolymerbiocomposites synthesisDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasCurrently, biopolymers have aroused great interest mainly in the food, pharmaceutical, cosmetic, and also in the biomedical fields, due to the intelligent and versatile properties that present, namely, biocompatibility, biodegradability, and non-toxicity. In addition to these properties, many polymers have vital characteristics that assist in wound healing processes and, when incorporated into nanoparticles of elements such as silver (Ag), gold (Au), and selenium (Se), further increase the effectiveness of these polymers. Therefore, the main objective of this study was to determine the best conditions for the preparation of biocomposites for application in the treatment of wounds, using different extracellular bacterial polysaccharides (EPSs) (EPS1, EPS2, EPS3, EPS4, and FucoPol) as reducing and stabilizing agents for the synthesis of silver nanoparticles (AgNP), gold (AuNP) and selenium (SeNP). EPSs were characterized as to the composition in sugars and substituting groups, because, depending on the composition, they can interfere in some way in the properties of each one. EPS1 is composed mainly of mannose (56.1% mol), EPS2 and EPS3 are composed mainly of glucose, 67% mol and 39.1% mol, respectively, EPS4 is composed mainly of glucuronic acid (55.9% mol) and FucoPol by fucose (34.9 mol%). Biocomposites were also characterized by UV-vis spectroscopy, FTIR, TGA, XRD, DLS, Zeta potential and ICP. Cytotoxicity and wound healing effect of EPSs and EPS/NPs biocomposites were analyzed in human keratinocyte cells (HaCaT cell line). The samples were tested using a polymer concentration of 62.5 - 1000 mg/L and none of the samples showed cytotoxicity, except for the EPS3/Ag+ biocomposite when tested at a polymer concentration above 125 mg/L, with a content Ag+ above 6.9 mg/L (decreased cell viability by ~91.9%). On the other hand, among all EPSs and their functionalized materials, FucoPol and the biocomposite FucoPol/AgNP exhibited a greater capacity to promote keratinocyte migration in vitro. Although EPS1 did not present wound healing effect, the biocomposites EPS1/AgNP (100 mg/L) and EPS1/AuNP (500 mg/L) showed to promote the migration of HaCaT cells. The results obtained suggest that both FucoPol and the biocomposite FucoPol/AgNP, as well as the biocomposites EPS1/AgNP and EPS1/AuNP, may have potential application in dermo-cosmetics, mainly in wound healing mechanisms.Atualmente, os biopolímeros têm despertado grande interesse principalmente na área alimentar, farmacêutica, cosmética e também na área biomédica, devido às propriedades inteligentes e versáteis que apresentam, nomeadamente, biocompatibilidade, biodegradabilidade e não toxicidade. Além dessas propriedades, muitos polímeros possuem características vitais que ajudam em processos de cicatrização de feridas e quando incorporados com nanopartículas de elementos como a prata (Ag), ouro (Au) e selénio (Se), aumentam ainda mais a eficácia desses polímeros. Assim sendo, o principal objetivo deste estudo foi determinar as melhores condições de preparação de biocompósitos para aplicação em tratamento de feridas, utilizando diferentes polissacáridos bacterianos extracelulares (EPSs) (EPS1, EPS2, EPS3, EPS4 e FucoPol) como agentes redutores e estabilizadores para a síntese de nanopartículas de prata (AgNP), ouro (AuNP) e selénio (SeNP). Os EPSs foram caracterizados quanto à composição em açúcares e grupos substituintes, pois, dependendo da composição, podem interferir de certa forma nas propriedades apresentadas por cada um. O EPS1 é composto principalmente por manose (56.1 %mol), o EPS2 e o EPS3 são compostos principalmente por glucose, 67 %mol e 39.1 %mol respectivamente, o EPS4 é composto maioritariamente por ácido glucurónico (55.9 %mol) e o FucoPol por fucose (34.9 %mol). Os biocompósitos também foram caracterizados por espectroscopia UV-vis, FTIR, TGA, XRD, DLS, potencial Zeta e por ICP. A citotoxicidade e o efeito de cicatrização de feridas dos EPS e dos biocompósitos EPSs/NPs foram analisados em células de queratinócitos humanos (linha celular HaCaT). As amostras foram testadas usando uma concentração de polímero de 62.5-1000 mg/L e nenhuma amostra apresentou citotoxicidade, excepto para o biocompósito EPS3/Ag+ quando testado em uma concentração de polímero acima de 125 mg/L, com um teor de Ag+ acima de 6.9 mg/L (diminuição da viabilidade cellular em ~ 91.9%). Por outro lado, entre todos os EPSs e seus materiais funcionalizados, o FucoPol e o biocompósito FucoPol/AgNP exibiram uma maior capacidade de promover a migração in vitro dos queratinócitos. Embora o EPS1 não tenha apresentado efeito cicatrizante, os biocompósitos EPS1/AgNP (100 mg/L) e EPS1/AuNP (500 mg/L) mostraram promover a migração de células HaCaT. Os resultados obtidos sugerem que tanto o FucoPol e o biocompósito FucoPol/AgNP, bem como os biocompósitos EPS1/AgNP e EPS1/AuNP podem ter potencial aplicação na dermocosmética, sobretudo nos mecanismos de cicatrização de feridas.Freitas, Maria FilomenaSerra, AnaRUNRamos, Kleyde Delsy Delgado2021-12-14T12:56:25Z2021-102021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/129170enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:25Zoai:run.unl.pt:10362/129170Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:29.093257Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
title |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
spellingShingle |
Development of wound healing biomaterials based on bacterial exopolysaccharides Ramos, Kleyde Delsy Delgado Exopolysaccharides nanoparticles cytotoxicity wound healing biopolymer biocomposites synthesis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
title_full |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
title_fullStr |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
title_full_unstemmed |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
title_sort |
Development of wound healing biomaterials based on bacterial exopolysaccharides |
author |
Ramos, Kleyde Delsy Delgado |
author_facet |
Ramos, Kleyde Delsy Delgado |
author_role |
author |
dc.contributor.none.fl_str_mv |
Freitas, Maria Filomena Serra, Ana RUN |
dc.contributor.author.fl_str_mv |
Ramos, Kleyde Delsy Delgado |
dc.subject.por.fl_str_mv |
Exopolysaccharides nanoparticles cytotoxicity wound healing biopolymer biocomposites synthesis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Exopolysaccharides nanoparticles cytotoxicity wound healing biopolymer biocomposites synthesis Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
Currently, biopolymers have aroused great interest mainly in the food, pharmaceutical, cosmetic, and also in the biomedical fields, due to the intelligent and versatile properties that present, namely, biocompatibility, biodegradability, and non-toxicity. In addition to these properties, many polymers have vital characteristics that assist in wound healing processes and, when incorporated into nanoparticles of elements such as silver (Ag), gold (Au), and selenium (Se), further increase the effectiveness of these polymers. Therefore, the main objective of this study was to determine the best conditions for the preparation of biocomposites for application in the treatment of wounds, using different extracellular bacterial polysaccharides (EPSs) (EPS1, EPS2, EPS3, EPS4, and FucoPol) as reducing and stabilizing agents for the synthesis of silver nanoparticles (AgNP), gold (AuNP) and selenium (SeNP). EPSs were characterized as to the composition in sugars and substituting groups, because, depending on the composition, they can interfere in some way in the properties of each one. EPS1 is composed mainly of mannose (56.1% mol), EPS2 and EPS3 are composed mainly of glucose, 67% mol and 39.1% mol, respectively, EPS4 is composed mainly of glucuronic acid (55.9% mol) and FucoPol by fucose (34.9 mol%). Biocomposites were also characterized by UV-vis spectroscopy, FTIR, TGA, XRD, DLS, Zeta potential and ICP. Cytotoxicity and wound healing effect of EPSs and EPS/NPs biocomposites were analyzed in human keratinocyte cells (HaCaT cell line). The samples were tested using a polymer concentration of 62.5 - 1000 mg/L and none of the samples showed cytotoxicity, except for the EPS3/Ag+ biocomposite when tested at a polymer concentration above 125 mg/L, with a content Ag+ above 6.9 mg/L (decreased cell viability by ~91.9%). On the other hand, among all EPSs and their functionalized materials, FucoPol and the biocomposite FucoPol/AgNP exhibited a greater capacity to promote keratinocyte migration in vitro. Although EPS1 did not present wound healing effect, the biocomposites EPS1/AgNP (100 mg/L) and EPS1/AuNP (500 mg/L) showed to promote the migration of HaCaT cells. The results obtained suggest that both FucoPol and the biocomposite FucoPol/AgNP, as well as the biocomposites EPS1/AgNP and EPS1/AuNP, may have potential application in dermo-cosmetics, mainly in wound healing mechanisms. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-14T12:56:25Z 2021-10 2021-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/129170 |
url |
http://hdl.handle.net/10362/129170 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138068418002944 |