Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/16601 https://doi.org/http://dx.doi.org/10.1016/j.cbpa.2015.07.018 https://doi.org/10.1016/j.cbpa.2015.07.018 |
Resumo: | Seawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells' basolateral membranes may disrupt the major transporter Na+K+-ATPase, the goal of this study was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membrane's phospholipids. When held in full-strength seawater, the fatty acid profile of basolateral membrane's phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na+K+- 2 ATPase revealed a significant and positive correlation with basolateral membrane's cholesterol content in the presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na+K+-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cell's basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey. |
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Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juvenilesbasolateral membranePetromyzon marinusgill cellsjuvenilesphospholipids compositionsaltwater acclimationSeawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells' basolateral membranes may disrupt the major transporter Na+K+-ATPase, the goal of this study was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membrane's phospholipids. When held in full-strength seawater, the fatty acid profile of basolateral membrane's phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na+K+- 2 ATPase revealed a significant and positive correlation with basolateral membrane's cholesterol content in the presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na+K+-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cell's basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey.This work was financially supported by the Foundation for Science and Technology (FCT) project PTDC/BIA-BEC/103258/2008 and project UID/MAR/04292/2013 (national funds FCT/MEC–PIDDAC through Acordo de Parceria PT2020).Comparative Biochemistry and Physiology2016-01-13T11:54:41Z2016-01-132015-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/16601https://doi.org/http://dx.doi.org/10.1016/j.cbpa.2015.07.018http://hdl.handle.net/10174/16601https://doi.org/10.1016/j.cbpa.2015.07.018engLança, M.J. et al., 2015 -Comparative Biochemistry and Physiology, Part A 189:67–75naonaonaoICAAMndmaria.potes.amaral.machado@gmail.comafferreira@fc.ul.ptbsquintella@fc.ul.ptpmra@uevora.pt361Lança, Maria JoãoMachado, MariaFerreira, FilipaQuintella, BernardoR. Almeida, Pedroinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:03:33Zoai:dspace.uevora.pt:10174/16601Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:08:58.828179Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
title |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
spellingShingle |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles Lança, Maria João basolateral membrane Petromyzon marinus gill cells juveniles phospholipids composition saltwater acclimation |
title_short |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
title_full |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
title_fullStr |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
title_full_unstemmed |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
title_sort |
Structural lipid changes and Na+/K+-ATPase activity of gill cells'basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles |
author |
Lança, Maria João |
author_facet |
Lança, Maria João Machado, Maria Ferreira, Filipa Quintella, Bernardo R. Almeida, Pedro |
author_role |
author |
author2 |
Machado, Maria Ferreira, Filipa Quintella, Bernardo R. Almeida, Pedro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lança, Maria João Machado, Maria Ferreira, Filipa Quintella, Bernardo R. Almeida, Pedro |
dc.subject.por.fl_str_mv |
basolateral membrane Petromyzon marinus gill cells juveniles phospholipids composition saltwater acclimation |
topic |
basolateral membrane Petromyzon marinus gill cells juveniles phospholipids composition saltwater acclimation |
description |
Seawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells' basolateral membranes may disrupt the major transporter Na+K+-ATPase, the goal of this study was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membrane's phospholipids. When held in full-strength seawater, the fatty acid profile of basolateral membrane's phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na+K+- 2 ATPase revealed a significant and positive correlation with basolateral membrane's cholesterol content in the presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na+K+-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cell's basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01T00:00:00Z 2016-01-13T11:54:41Z 2016-01-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/16601 https://doi.org/http://dx.doi.org/10.1016/j.cbpa.2015.07.018 http://hdl.handle.net/10174/16601 https://doi.org/10.1016/j.cbpa.2015.07.018 |
url |
http://hdl.handle.net/10174/16601 https://doi.org/http://dx.doi.org/10.1016/j.cbpa.2015.07.018 https://doi.org/10.1016/j.cbpa.2015.07.018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lança, M.J. et al., 2015 -Comparative Biochemistry and Physiology, Part A 189:67–75 nao nao nao ICAAM nd maria.potes.amaral.machado@gmail.com afferreira@fc.ul.pt bsquintella@fc.ul.pt pmra@uevora.pt 361 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Comparative Biochemistry and Physiology |
publisher.none.fl_str_mv |
Comparative Biochemistry and Physiology |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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