Quantitative assessment of individual populations within polymicrobial biofilms

Detalhes bibliográficos
Autor(a) principal: Lopes, Susana P.
Data de Publicação: 2018
Outros Autores: Azevedo, Nuno Filipe, Pereira, Maria Olívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/55170
Resumo: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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spelling Quantitative assessment of individual populations within polymicrobial biofilmsScience & TechnologyThe datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.Selecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and heterogeneous nature. Here, plate count, quantitative real-time polymerase chain reaction (q-PCR) and peptide nucleic acid probe-fluorescence in situ hybridization (PNA-FISH) were employed to quantitate cystic fibrosis multispecies biofilms. Growth of Pseudomonas aeruginosa, Inquilinus limosus and Dolosigranulum pigrum was assessed in dual- and triple-species consortia under oxygen and antibiotic stress. Quantification methods, that were previously optimized and validated in planktonic consortia, were not always in agreement when applied in multispecies biofilms. Discrepancies in culture and molecular outcomes were observed, particularly for triple-species consortia and antibiotic-stressed biofilms. Some differences were observed, such as the higher bacterial counts obtained by q-PCR and/or PNA-FISH (?4 log10 cells/cm2) compared to culture. But the discrepancies between PNA-FISH and q-PCR data (eg D. pigrum limited assessment by q-PCR) demonstrate the effect of biofilm heterogeneity in method's reliability. As the heterogeneity in biofilms is a reflection of a myriad of variables, tailoring an accurate picture of communities? changes is crucial. This work demonstrates that at least two, but preferentially three, quantification techniques are required to obtain reliable measures and take comprehensive analysis of polymicrobial biofilm-associated infections.The authors thank the financial support from the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI-01–0145-FEDER-006684) and BioTecNorte operation (NORTE01-0145-FEDER-000004) funded by the European Regional Development Fund (ERDF) under the scope of Norte2020 - Programa Operacional Regional do Norte. This work was also the result of the projects: (i) POCI01-0145-FEDER-006939 (Laboratory for Processing Engineering, Environment, Biotechnology and Energy – UID/EQU/00511/2013) funded by the ERDF, through COMPETE2020 and by national funds, through FCT; (ii) NORTE-01-0145-FEDER-000005 – LEPABE-2-ECO-INNOVATION, supported by North Portugal Regional Operational Programme (NORTE2020), under the Portugal2020 Partnership Agreement, through the ERDF; (iii) Coded-FISH PTDC/DTP-PIC/4562/2014/16678; (iv) POCI-01-0145-FEDER-029841, through COMPETE2020 - Programa Operacional Competitividade e Internacionalização and by national funds, through FCT). Also, the fellowship of Susana P. Lopes SFRH/BPD/95616/2013 is acknowledged. The authors would also like to thanks to Dr Michael Surette (Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada) for kindly providing the I. limosus strain used in this study.info:eu-repo/semantics/publishedVersionNature GroupUniversidade do MinhoLopes, Susana P.Azevedo, Nuno FilipePereira, Maria Olívia2018-06-222018-06-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55170engLopes, Susana P.; Azevedo, Nuno Filipe; Pereira, Maria Olívia, Quantitative assessment of individual populations within polymicrobial biofilms. Scientific Reports, 8(9494), 20182045-23222045-232210.1038/s41598-018-27497-929934504http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:44Zoai:repositorium.sdum.uminho.pt:1822/55170Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:23.584728Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Quantitative assessment of individual populations within polymicrobial biofilms
title Quantitative assessment of individual populations within polymicrobial biofilms
spellingShingle Quantitative assessment of individual populations within polymicrobial biofilms
Lopes, Susana P.
Science & Technology
title_short Quantitative assessment of individual populations within polymicrobial biofilms
title_full Quantitative assessment of individual populations within polymicrobial biofilms
title_fullStr Quantitative assessment of individual populations within polymicrobial biofilms
title_full_unstemmed Quantitative assessment of individual populations within polymicrobial biofilms
title_sort Quantitative assessment of individual populations within polymicrobial biofilms
author Lopes, Susana P.
author_facet Lopes, Susana P.
Azevedo, Nuno Filipe
Pereira, Maria Olívia
author_role author
author2 Azevedo, Nuno Filipe
Pereira, Maria Olívia
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lopes, Susana P.
Azevedo, Nuno Filipe
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-22
2018-06-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/55170
url http://hdl.handle.net/1822/55170
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lopes, Susana P.; Azevedo, Nuno Filipe; Pereira, Maria Olívia, Quantitative assessment of individual populations within polymicrobial biofilms. Scientific Reports, 8(9494), 2018
2045-2322
2045-2322
10.1038/s41598-018-27497-9
29934504
http://www.nature.com/srep/index.html
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Group
publisher.none.fl_str_mv Nature Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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