Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy

Detalhes bibliográficos
Autor(a) principal: Nunes, Jéssica Lopes
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/6686
Resumo: There is growing evidence of an important role of epigenetic on the progression of neurodegenerative diseases, like Parkinson’s disease (PD). C-terminal binding proteins (CtBPs) are transcriptional co-repressors that exert transcriptional repression primarily via recruitment of a co-repressor complex to DNA. Some studies have demonstrated a critical function for CtBP1 and CtBP2 in the transcriptional repression of pro-apoptotic genes, suggesting being good therapeutic target for neurodegenerative diseases. Herein, we explored the expression of CtBPs in in vitro and in vivo models for PD by western-blotting, and their putative effect on dopaminergic survival by the MTT assay (in vitro) or by tyrosine hydroxylase cell countings (in vivo). First, increased expression of CtBP1 was found in the substantia nigra (SN) of 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) challenged mice, while a significant increased expression was found in the striatum (ST) of mice challenged with 6-OHDA only. CtBP2 expression was increased both in the SN and ST of 6-OHDA treated mice, although not reaching statistical significance when compared with saline mice. In accordance, the expression of both CtBP1 and CtBP2 was increased in a rat dopaminergic neural cell line (N27) exposed to 6-OHDA. Then, a broad antagonist of CtBPs, the 4-methylthio 2-oxobutyric acid (MTOB), was used to assess the putative role of CtBPs on neuronal survival. MTOB, at relatively high concentrations, was able to inhibit dopaminergic survival in N27 cells and in the SN of 6-OHDA in vivo mouse model for PD. Moreover, MTOB was able to potentiate cell death induced by 1-methyl-4-phenylpyridinium (MPP+). Interestingly, low doses of MTOB (250 µM) were able to counteract cell death induced by 6-OHDA in in vitro and in vivo PD models. Altogether, our results suggest that CtBPs are a good target to study transcriptional regulation mechanisms that modulate dopaminergic survival.
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spelling Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy6-OhdaDoença de ParkinsonMtobProteínas de Ligação C-TerminalSobrevivência DopaminérgicaDomínio/Área Científica::Ciências Médicas::Ciências BiomédicasThere is growing evidence of an important role of epigenetic on the progression of neurodegenerative diseases, like Parkinson’s disease (PD). C-terminal binding proteins (CtBPs) are transcriptional co-repressors that exert transcriptional repression primarily via recruitment of a co-repressor complex to DNA. Some studies have demonstrated a critical function for CtBP1 and CtBP2 in the transcriptional repression of pro-apoptotic genes, suggesting being good therapeutic target for neurodegenerative diseases. Herein, we explored the expression of CtBPs in in vitro and in vivo models for PD by western-blotting, and their putative effect on dopaminergic survival by the MTT assay (in vitro) or by tyrosine hydroxylase cell countings (in vivo). First, increased expression of CtBP1 was found in the substantia nigra (SN) of 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) challenged mice, while a significant increased expression was found in the striatum (ST) of mice challenged with 6-OHDA only. CtBP2 expression was increased both in the SN and ST of 6-OHDA treated mice, although not reaching statistical significance when compared with saline mice. In accordance, the expression of both CtBP1 and CtBP2 was increased in a rat dopaminergic neural cell line (N27) exposed to 6-OHDA. Then, a broad antagonist of CtBPs, the 4-methylthio 2-oxobutyric acid (MTOB), was used to assess the putative role of CtBPs on neuronal survival. MTOB, at relatively high concentrations, was able to inhibit dopaminergic survival in N27 cells and in the SN of 6-OHDA in vivo mouse model for PD. Moreover, MTOB was able to potentiate cell death induced by 1-methyl-4-phenylpyridinium (MPP+). Interestingly, low doses of MTOB (250 µM) were able to counteract cell death induced by 6-OHDA in in vitro and in vivo PD models. Altogether, our results suggest that CtBPs are a good target to study transcriptional regulation mechanisms that modulate dopaminergic survival.Evidências sugerem um papel epigenético associado à progressão de doenças neurodegenerativas, tais como a doença de Parkinson (PD, do inglês Parkinson’s disease). As proteínas de ligação C-terminal (CtBPs, do inglês C-terminal binding proteins) são co-repressores transcripcionais, que atuam, essencialmente, através do recrutamento de um complexo co-repressor ao ADN. Alguns estudos demonstraram uma função importante para as CtBPs na repressão da transcrição de genes pró-apoptóticos, demonstrando ser um bom alvo terapêutico em doenças neurodegenerativas. Neste trabalho, explorámos a expressão proteica das CtBPs em modelos in vitro e in vivo da PD através de western-blot e o seu efeito na sobrevivência dopaminérgica através de ensaios de MTT (in vitro) ou por contagem do número de células que expressam tirosina hidroxilase (TH) (in vivo). Em primeiro lugar, verificou-se um aumento de expressão de CtBP1 na substantia nigra (SN) nos animais injetados com 6-hidroxidopamina (6-OHDA) ou 1-metil-4-fenil-1,2,3,6-tetrahidropiridina (MPTP), no entanto no estriado (ST, do inglês striatum) apenas se verificou uma diferença estatisticamente significativa nos animais injetados com 6-OHDA, quando comparados com os animais salinos. Os níveis de expressão da CtBP2 na SN e no ST aumentaram após a injeção da 6-OHDA, no entanto não de uma forma estatisticamente significativa quando comparados com os resultados obtidos nas mesmas regiões em animais salinos. Concordantemente, tanto a expressão da CtBP1 como da CtBP2 aumentou numa linha neural dopaminérgica do mesencéfalo de ratos (N27) exposta à toxina 6-OHDA. Seguidamente, utilizou-se um antagonista das CtBPs, o ácido 4-metiltio-2-oxobutírico (MTOB), para determinar o efeito putativo das CtBPs na sobrevivência neuronal. O MTOB, a concentrações relativamente elevadas, foi capaz de inibir a sobrevivência neuronal das células N27 e a sobrevivência dopaminérgica na SN do modelo animal induzido por 6-OHDA. Adicionalmente, o MTOB foi capaz de potenciar a morte celular induzida por 1-metil-4-fenilpiridinio (MPP+). Curiosamente, baixas concentrações de MTOB (250µM) foram capazes de contrariar a morte celular induzida pela 6-OHDA nos modelos da PD in vitro e in vivo. Concluindo, os nossos resultados sugerem que as CtBPs são um bom alvo para se estudar mecanismos de regulação transcripcional na modulação da sobrevivência dopaminérgica.Bernardino, Liliana InáciouBibliorumNunes, Jéssica Lopes2020-06-26T00:30:11Z2017-6-272017-07-132017-07-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.6/6686TID:202107540enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:45:24Zoai:ubibliorum.ubi.pt:10400.6/6686Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:47:21.285180Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
title Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
spellingShingle Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
Nunes, Jéssica Lopes
6-Ohda
Doença de Parkinson
Mtob
Proteínas de Ligação C-Terminal
Sobrevivência Dopaminérgica
Domínio/Área Científica::Ciências Médicas::Ciências Biomédicas
title_short Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
title_full Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
title_fullStr Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
title_full_unstemmed Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
title_sort Novel transcriptional regulation mechanisms associated with Parkinson’s disease pathogenesis: from biological activity towards therapy
author Nunes, Jéssica Lopes
author_facet Nunes, Jéssica Lopes
author_role author
dc.contributor.none.fl_str_mv Bernardino, Liliana Inácio
uBibliorum
dc.contributor.author.fl_str_mv Nunes, Jéssica Lopes
dc.subject.por.fl_str_mv 6-Ohda
Doença de Parkinson
Mtob
Proteínas de Ligação C-Terminal
Sobrevivência Dopaminérgica
Domínio/Área Científica::Ciências Médicas::Ciências Biomédicas
topic 6-Ohda
Doença de Parkinson
Mtob
Proteínas de Ligação C-Terminal
Sobrevivência Dopaminérgica
Domínio/Área Científica::Ciências Médicas::Ciências Biomédicas
description There is growing evidence of an important role of epigenetic on the progression of neurodegenerative diseases, like Parkinson’s disease (PD). C-terminal binding proteins (CtBPs) are transcriptional co-repressors that exert transcriptional repression primarily via recruitment of a co-repressor complex to DNA. Some studies have demonstrated a critical function for CtBP1 and CtBP2 in the transcriptional repression of pro-apoptotic genes, suggesting being good therapeutic target for neurodegenerative diseases. Herein, we explored the expression of CtBPs in in vitro and in vivo models for PD by western-blotting, and their putative effect on dopaminergic survival by the MTT assay (in vitro) or by tyrosine hydroxylase cell countings (in vivo). First, increased expression of CtBP1 was found in the substantia nigra (SN) of 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) challenged mice, while a significant increased expression was found in the striatum (ST) of mice challenged with 6-OHDA only. CtBP2 expression was increased both in the SN and ST of 6-OHDA treated mice, although not reaching statistical significance when compared with saline mice. In accordance, the expression of both CtBP1 and CtBP2 was increased in a rat dopaminergic neural cell line (N27) exposed to 6-OHDA. Then, a broad antagonist of CtBPs, the 4-methylthio 2-oxobutyric acid (MTOB), was used to assess the putative role of CtBPs on neuronal survival. MTOB, at relatively high concentrations, was able to inhibit dopaminergic survival in N27 cells and in the SN of 6-OHDA in vivo mouse model for PD. Moreover, MTOB was able to potentiate cell death induced by 1-methyl-4-phenylpyridinium (MPP+). Interestingly, low doses of MTOB (250 µM) were able to counteract cell death induced by 6-OHDA in in vitro and in vivo PD models. Altogether, our results suggest that CtBPs are a good target to study transcriptional regulation mechanisms that modulate dopaminergic survival.
publishDate 2017
dc.date.none.fl_str_mv 2017-6-27
2017-07-13
2017-07-13T00:00:00Z
2020-06-26T00:30:11Z
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