Electrochemical behaviour of cytochrome c at electrically heated microelectrodes
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5270 https://doi.org/10.1016/S0731-7085(98)00189-7 |
Resumo: | The structural changes in cytochrome c with temperature have been been followed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurements of cytochrome c oxidation at different temperatures at heated bare gold electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a marked dependence of the signal on electrode temperature. This augurs well for applications of heated electrodes as local probes in the study of the temperature dependence of electron transfer processes of other redox proteins, avoiding problems of bulk deterioration. |
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Electrochemical behaviour of cytochrome c at electrically heated microelectrodesElectrochemistry of cytochrome cHot-wire electrodesProtein unfoldingThe structural changes in cytochrome c with temperature have been been followed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurements of cytochrome c oxidation at different temperatures at heated bare gold electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a marked dependence of the signal on electrode temperature. This augurs well for applications of heated electrodes as local probes in the study of the temperature dependence of electron transfer processes of other redox proteins, avoiding problems of bulk deterioration.http://www.sciencedirect.com/science/article/B6TGX-3VKCCRN-D/1/171b8580ef00f2e0f90392182b57bde71999info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5270http://hdl.handle.net/10316/5270https://doi.org/10.1016/S0731-7085(98)00189-7engJournal of Pharmaceutical and Biomedical Analysis. 19:1-2 (1999) 127-133Voss, T.Gründler, P.Brett, C. M. A.Brett, A. M. Oliveirainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-07-27T09:44:06Zoai:estudogeral.uc.pt:10316/5270Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:38.320412Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
title |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
spellingShingle |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes Voss, T. Electrochemistry of cytochrome c Hot-wire electrodes Protein unfolding |
title_short |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
title_full |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
title_fullStr |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
title_full_unstemmed |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
title_sort |
Electrochemical behaviour of cytochrome c at electrically heated microelectrodes |
author |
Voss, T. |
author_facet |
Voss, T. Gründler, P. Brett, C. M. A. Brett, A. M. Oliveira |
author_role |
author |
author2 |
Gründler, P. Brett, C. M. A. Brett, A. M. Oliveira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Voss, T. Gründler, P. Brett, C. M. A. Brett, A. M. Oliveira |
dc.subject.por.fl_str_mv |
Electrochemistry of cytochrome c Hot-wire electrodes Protein unfolding |
topic |
Electrochemistry of cytochrome c Hot-wire electrodes Protein unfolding |
description |
The structural changes in cytochrome c with temperature have been been followed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurements of cytochrome c oxidation at different temperatures at heated bare gold electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a marked dependence of the signal on electrode temperature. This augurs well for applications of heated electrodes as local probes in the study of the temperature dependence of electron transfer processes of other redox proteins, avoiding problems of bulk deterioration. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5270 http://hdl.handle.net/10316/5270 https://doi.org/10.1016/S0731-7085(98)00189-7 |
url |
http://hdl.handle.net/10316/5270 https://doi.org/10.1016/S0731-7085(98)00189-7 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pharmaceutical and Biomedical Analysis. 19:1-2 (1999) 127-133 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133907388465152 |