Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

Detalhes bibliográficos
Autor(a) principal: Costa, Cristina
Data de Publicação: 1996
Outros Autores: Moura, José J. G., Moura, Isabel, Wang, Yaning, Huynh, Boi Hanh
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1074/jbc.271.38.23191
Resumo: NIGMS NIH HHS (GM 39803) NIGMS NIH HHS (GM 47295)
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spelling Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774BiochemistryMolecular BiologyCell BiologyNIGMS NIH HHS (GM 39803) NIGMS NIH HHS (GM 47295)The dissimilatory nitrite reductase from Desulfovibrio desulfuricans ATCC 27774 catalyzes the reduction of nitrite to ammonia. Previous spectroscopic investigation revealed that it is a hexaheme cytochrome containing one high spin ferric heme and five low spin ferric hemes in the oxidized enzyme. The current study uses the high resolution of Mossbauer spectroscopy to obtain redox properties of the six heme groups. Correlating the Mossbauer findings with the EPR data reveals the pair-wise spin-spin coupling among four of the heme groups. The other two hemes are found to be magnetically isolated. Reduction with dithionite and reaction with CO further indicate that only the high spin heme is capable of binding small exogenous ligands. These results confirm our previous finding that Desulfovibrio desulfuricans nitrite reductase contains six heme groups and that the high spin ferric heme is the substrate and inhibitor binding site.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNCosta, CristinaMoura, José J. G.Moura, IsabelWang, YaningHuynh, Boi Hanh2019-09-09T22:37:06Z1996-09-301996-09-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttps://doi.org/10.1074/jbc.271.38.23191eng0021-9258PURE: 14620899http://www.scopus.com/inward/record.url?scp=0029841950&partnerID=8YFLogxKhttps://doi.org/10.1074/jbc.271.38.23191info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:35Zoai:run.unl.pt:10362/80624Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:52.889776Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
title Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
spellingShingle Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
Costa, Cristina
Biochemistry
Molecular Biology
Cell Biology
title_short Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
title_full Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
title_fullStr Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
title_full_unstemmed Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
title_sort Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774
author Costa, Cristina
author_facet Costa, Cristina
Moura, José J. G.
Moura, Isabel
Wang, Yaning
Huynh, Boi Hanh
author_role author
author2 Moura, José J. G.
Moura, Isabel
Wang, Yaning
Huynh, Boi Hanh
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Costa, Cristina
Moura, José J. G.
Moura, Isabel
Wang, Yaning
Huynh, Boi Hanh
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Cell Biology
topic Biochemistry
Molecular Biology
Cell Biology
description NIGMS NIH HHS (GM 39803) NIGMS NIH HHS (GM 47295)
publishDate 1996
dc.date.none.fl_str_mv 1996-09-30
1996-09-30T00:00:00Z
2019-09-09T22:37:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1074/jbc.271.38.23191
url https://doi.org/10.1074/jbc.271.38.23191
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9258
PURE: 14620899
http://www.scopus.com/inward/record.url?scp=0029841950&partnerID=8YFLogxK
https://doi.org/10.1074/jbc.271.38.23191
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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