GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/732 |
Resumo: | The non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth. Until now it was not known whether GABA exerts its effects in plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux through plant aluminium-activated malate transporter (ALMT) proteins is activated by anions and negatively regulated by GABA. Site-directed mutagenesis of selected amino acids within ALMT proteins abolishes GABA efficacy but does not alter other transport properties. GABA modulation of ALMT activity results in altered root growth and altered root tolerance to alkaline pH, acid pH and aluminium ions. We propose that GABA exerts its multiple physiological effects in plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally be considered a legitimate signalling molecule in both the plant and animal kingdoms. |
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GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transportersPlant physiologyPlant signallingPlant transportersThe non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth. Until now it was not known whether GABA exerts its effects in plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux through plant aluminium-activated malate transporter (ALMT) proteins is activated by anions and negatively regulated by GABA. Site-directed mutagenesis of selected amino acids within ALMT proteins abolishes GABA efficacy but does not alter other transport properties. GABA modulation of ALMT activity results in altered root growth and altered root tolerance to alkaline pH, acid pH and aluminium ions. We propose that GABA exerts its multiple physiological effects in plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally be considered a legitimate signalling molecule in both the plant and animal kingdoms.Australian Research Council; Waite Research Institute grants: (FT130100709, DP130104205, CE140100008); Fundação para a Ciência e Tecnologia grant: (PTDC/BEX-BCM/0376/2012); University of Maryland.Nature Publishing GroupARCARamesh, Sunita A.Tyerman, Stephen D.Xu, BoBose, JayakumarKaur, SatwinderConn, VanessaDomingos, PatriciaUllah, SanaWege, StefanieShabala, SergeyFeijó, José A.Ryan, Peter R.Gillham, Matthew2017-02-15T18:16:49Z2015-07-292015-07-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/732engRamesh, S. A. et al. GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters. Nat. Commun. 6:7879 doi: 10.1038/ncomms8879 (2015).10.1038/ncomms8879info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:07Zoai:arca.igc.gulbenkian.pt:10400.7/732Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:57.729127Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
title |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
spellingShingle |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters Ramesh, Sunita A. Plant physiology Plant signalling Plant transporters |
title_short |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
title_full |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
title_fullStr |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
title_full_unstemmed |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
title_sort |
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters |
author |
Ramesh, Sunita A. |
author_facet |
Ramesh, Sunita A. Tyerman, Stephen D. Xu, Bo Bose, Jayakumar Kaur, Satwinder Conn, Vanessa Domingos, Patricia Ullah, Sana Wege, Stefanie Shabala, Sergey Feijó, José A. Ryan, Peter R. Gillham, Matthew |
author_role |
author |
author2 |
Tyerman, Stephen D. Xu, Bo Bose, Jayakumar Kaur, Satwinder Conn, Vanessa Domingos, Patricia Ullah, Sana Wege, Stefanie Shabala, Sergey Feijó, José A. Ryan, Peter R. Gillham, Matthew |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Ramesh, Sunita A. Tyerman, Stephen D. Xu, Bo Bose, Jayakumar Kaur, Satwinder Conn, Vanessa Domingos, Patricia Ullah, Sana Wege, Stefanie Shabala, Sergey Feijó, José A. Ryan, Peter R. Gillham, Matthew |
dc.subject.por.fl_str_mv |
Plant physiology Plant signalling Plant transporters |
topic |
Plant physiology Plant signalling Plant transporters |
description |
The non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth. Until now it was not known whether GABA exerts its effects in plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux through plant aluminium-activated malate transporter (ALMT) proteins is activated by anions and negatively regulated by GABA. Site-directed mutagenesis of selected amino acids within ALMT proteins abolishes GABA efficacy but does not alter other transport properties. GABA modulation of ALMT activity results in altered root growth and altered root tolerance to alkaline pH, acid pH and aluminium ions. We propose that GABA exerts its multiple physiological effects in plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally be considered a legitimate signalling molecule in both the plant and animal kingdoms. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-29 2015-07-29T00:00:00Z 2017-02-15T18:16:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/732 |
url |
http://hdl.handle.net/10400.7/732 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ramesh, S. A. et al. GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters. Nat. Commun. 6:7879 doi: 10.1038/ncomms8879 (2015). 10.1038/ncomms8879 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130574755987456 |