RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing

Detalhes bibliográficos
Autor(a) principal: Nojima, Takayuki
Data de Publicação: 2018
Outros Autores: Rebelo, Kenny, Gomes, Tomás, Grosso, Ana Rita, Proudfoot, Nicholas J., Carmo-Fonseca, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49190
Resumo: © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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spelling RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicingRNA polymerase IISpliceosomeCo-transcriptional splicingExon definitionmNET-MSmNET-seqPhospho-serine 5 CTDSplicing intermediates© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).The highly intronic nature of protein coding genes in mammals necessitates a co-transcriptional splicing mechanism as revealed by mNET-seq analysis. Immunoprecipitation of MNase-digested chromatin with antibodies against RNA polymerase II (Pol II) shows that active spliceosomes (both snRNA and proteins) are complexed to Pol II S5P CTD during elongation and co-transcriptional splicing. Notably, elongating Pol II-spliceosome complexes form strong interactions with nascent transcripts, resulting in footprints of approximately 60 nucleotides. Also, splicing intermediates formed by cleavage at the 5' splice site are associated with nearly all spliced exons. These spliceosome-bound intermediates are frequently ligated to upstream exons, implying a sequential, constitutive, and U12-dependent splicing process. Finally, lack of detectable spliced products connected to the Pol II active site in human HeLa or murine lymphoid cells suggests that splicing does not occur immediately following 3' splice site synthesis. Our results imply that most mammalian splicing requires exon definition for completion.This work was supported by funding to N.J.P. (Wellcome Trust Investigator Award [107928/Z/15/Z] and ERC Advanced [339270] grants) and to M.C.-F. (Fundação Ciência e Tecnologia, Portugal; grant PTDC/BEX-BCM/5899/2014).ElsevierRepositório da Universidade de LisboaNojima, TakayukiRebelo, KennyGomes, TomásGrosso, Ana RitaProudfoot, Nicholas J.Carmo-Fonseca, Maria2021-07-28T14:20:13Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49190engMol Cell. 2018 Oct 18;72(2):369-379.e41097-276510.1016/j.molcel.2018.09.0041097-4164info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:40Zoai:repositorio.ul.pt:10451/49190Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:48.877016Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
title RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
spellingShingle RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
Nojima, Takayuki
RNA polymerase II
Spliceosome
Co-transcriptional splicing
Exon definition
mNET-MS
mNET-seq
Phospho-serine 5 CTD
Splicing intermediates
title_short RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
title_full RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
title_fullStr RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
title_full_unstemmed RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
title_sort RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing
author Nojima, Takayuki
author_facet Nojima, Takayuki
Rebelo, Kenny
Gomes, Tomás
Grosso, Ana Rita
Proudfoot, Nicholas J.
Carmo-Fonseca, Maria
author_role author
author2 Rebelo, Kenny
Gomes, Tomás
Grosso, Ana Rita
Proudfoot, Nicholas J.
Carmo-Fonseca, Maria
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Nojima, Takayuki
Rebelo, Kenny
Gomes, Tomás
Grosso, Ana Rita
Proudfoot, Nicholas J.
Carmo-Fonseca, Maria
dc.subject.por.fl_str_mv RNA polymerase II
Spliceosome
Co-transcriptional splicing
Exon definition
mNET-MS
mNET-seq
Phospho-serine 5 CTD
Splicing intermediates
topic RNA polymerase II
Spliceosome
Co-transcriptional splicing
Exon definition
mNET-MS
mNET-seq
Phospho-serine 5 CTD
Splicing intermediates
description © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2021-07-28T14:20:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49190
url http://hdl.handle.net/10451/49190
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mol Cell. 2018 Oct 18;72(2):369-379.e4
1097-2765
10.1016/j.molcel.2018.09.004
1097-4164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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