HMB-45 AND KI-67 STAINING FOR MELANOMA ASSOCIATED WITH NEVI

• Autor(a) principal: Gomes, Joana
• Data de Publicação: 2011
• Outros Autores: Parente, Joana, Viana, Isabel, Vale, Esmeralda
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://doi.org/10.29021/spdv.69.2.605
• topic: Melanoma; Nevi; HMB-45; Ki-67; Immunohistochemestry; Melanoma; Nevo; HMB-45; Ki-67; Imunohistoquímica

Introduction: The majority of melanomas appear to arise de novo, however in about one third of the cases it can arise in association with a preexisting nevus. The histopathological diagnosis in these situations can be difficult, particularly in the separation of the two cellular populations and, as a consequence, establishing the tumoral thickness. Immunohistochemical staining can help in this distinction.Objectives: To evaluate the immunohistochemical staining patterns of HMB-45 and Ki-67 in selected cases of malignant melanoma associated with melanocytic nevi.Methods: Immunoperoxidase stains for HMB-45 and Ki-67 were done on 5 dermal melanocytic nevi, 5 compound melanocytic nevi, 5 invasive melanomas and 16 melanomas arising in a nevus.Results: The dermal melanocytes of nevi were consistently negative for HMB-45 and Ki-67. The melanomas showed diffuse moderate to intense staining for HMB-45 and Ki-65. In melanomas associated with nevus the HMB-45 was consistently positive in the melanoma component and negative in the nevus, allowing the separation of the two cellular populations. We find a considerable variation in the Ki-67 staining in this group.Conclusion: Although the histopathological examination with hematoxilin and eosin remains the main component in the diagnosis of melanocytic lesions; the immunohistochemical staining with HMB-45 and Ki-67 can be helpful in the diagnosis of melanoma associated with nevi.