Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches

Detalhes bibliográficos
Autor(a) principal: Guerreiro, Marco Alexandre Figueira Rodrigues
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8288
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approachesFungiBasidiomycetesCryptococcusMAT locusSexual reproductionMLSTDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaIn fungi, sexual reproduction is orchestrated by genomic regions known as mating type loci (MAT), which can be defined by two physically unlinked sex determining regions (tetrapolar mating system) or a single locus (bipolar system). Kwoniella mangroviensis is a saprobic basidiomycetous yeast, belonging to the Kwoniella clade of the order Tremellales, which was described as possessing a bipolar mating-system, similar to the related pathogenic species Cryptococcus neoformans and Cryptococcus gattii of the sister Filobasidiella clade. Studies aimed at elucidating the evolution of the MAT locus of these two Cryptococcus species of clinical importance, targeted several related saprobic species in the Kwoniella and Filobasidiella clades. An evolutionary model ensuing from those studies suggests that the tetrapolar mating-systems found in most species are ancestral and gave rise to the bipolar mating-system of C. neoformans by chromosomal rearrangements and fusion events. The present study comprised strains from the original work describing K. mangroviensis, as well as additional isolates from plant substrates in Europe and Africa. A multilocus sequence typing approach revealed genetic variability among those strains and led to the identification of two novel species closely related to K. mangroviensis: Kwoniella sp. A and Kwoniella sp. B. The mating system of K. mangroviensis and sibling species was further explored by a genetic approach based on sequencing of two MAT genes: STE20 and the divergently transcribed genes SXI1 and SXI2. The results obtained demonstrated tetrapolar mating systems in K. mangroviensis, as well as in Kwoniella sp. A and Kwoniella sp. B. Additionally, the MAT locus structure of K. mangroviensis was unveiled by sequencing a 43 kb genomic region containing the STE20 gene. Twelve genes also present in the MAT loci of related species were identified, and full synteny was found between K. mangroviensis and Cryptococcus heveanensis, a distant member of the Kwoniella clade. These findings provided novel insights into the evolution of MAT loci in basidiomycetous yeasts in the Tremellales.Fundação para a Ciência e Tecnologia - PTDC/BIA-MIC/113051/2009Faculdade de Ciências e TecnologiaFonseca, ÁlvaroRUNGuerreiro, Marco Alexandre Figueira Rodrigues2012-12-04T10:24:08Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/8288enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:40:51Zoai:run.unl.pt:10362/8288Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:06.862632Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
title Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
spellingShingle Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
Guerreiro, Marco Alexandre Figueira Rodrigues
Fungi
Basidiomycetes
Cryptococcus
MAT locus
Sexual reproduction
MLST
title_short Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
title_full Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
title_fullStr Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
title_full_unstemmed Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
title_sort Unveiling the mating system and genetic variability in the yeast Kwoniella mangroviensis using molecular approaches
author Guerreiro, Marco Alexandre Figueira Rodrigues
author_facet Guerreiro, Marco Alexandre Figueira Rodrigues
author_role author
dc.contributor.none.fl_str_mv Fonseca, Álvaro
RUN
dc.contributor.author.fl_str_mv Guerreiro, Marco Alexandre Figueira Rodrigues
dc.subject.por.fl_str_mv Fungi
Basidiomycetes
Cryptococcus
MAT locus
Sexual reproduction
MLST
topic Fungi
Basidiomycetes
Cryptococcus
MAT locus
Sexual reproduction
MLST
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2012
dc.date.none.fl_str_mv 2012-12-04T10:24:08Z
2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8288
url http://hdl.handle.net/10362/8288
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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