Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/31242 |
Resumo: | The Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products. |
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Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breedsAlentejano pigGene variabilityDEGsmeat qualityBísaro piglipid metabolismThe Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products.2022-03-07T12:28:14Z2022-03-072020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/31242http://hdl.handle.net/10174/31242engAlbuquerque, A.; M.R. Félix; M. Laranjo; R. Charneca; J.M. Martins (2020). Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds. In: V PhD Students Meeting in Environmental and Agriculture – Book of Abstracts, Pólo da Mitra, Valverde, Portugal, p. 17.ZOOandrealb@uevora.ptndndndjmartins@uevora.pt226Albuquerque, A.Félix, M.R.Laranjo, M.Charneca, R.Martins, J.M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:30:35Zoai:dspace.uevora.pt:10174/31242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:20:28.056868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
title |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
spellingShingle |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds Albuquerque, A. Alentejano pig Gene variability DEGs meat quality Bísaro pig lipid metabolism |
title_short |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
title_full |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
title_fullStr |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
title_full_unstemmed |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
title_sort |
Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds |
author |
Albuquerque, A. |
author_facet |
Albuquerque, A. Félix, M.R. Laranjo, M. Charneca, R. Martins, J.M. |
author_role |
author |
author2 |
Félix, M.R. Laranjo, M. Charneca, R. Martins, J.M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Albuquerque, A. Félix, M.R. Laranjo, M. Charneca, R. Martins, J.M. |
dc.subject.por.fl_str_mv |
Alentejano pig Gene variability DEGs meat quality Bísaro pig lipid metabolism |
topic |
Alentejano pig Gene variability DEGs meat quality Bísaro pig lipid metabolism |
description |
The Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01T00:00:00Z 2022-03-07T12:28:14Z 2022-03-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/31242 http://hdl.handle.net/10174/31242 |
url |
http://hdl.handle.net/10174/31242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Albuquerque, A.; M.R. Félix; M. Laranjo; R. Charneca; J.M. Martins (2020). Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds. In: V PhD Students Meeting in Environmental and Agriculture – Book of Abstracts, Pólo da Mitra, Valverde, Portugal, p. 17. ZOO andrealb@uevora.pt nd nd nd jmartins@uevora.pt 226 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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