Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds

Detalhes bibliográficos
Autor(a) principal: Albuquerque, A.
Data de Publicação: 2020
Outros Autores: Félix, M.R., Laranjo, M., Charneca, R., Martins, J.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/31242
Resumo: The Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products.
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spelling Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breedsAlentejano pigGene variabilityDEGsmeat qualityBísaro piglipid metabolismThe Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products.2022-03-07T12:28:14Z2022-03-072020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/31242http://hdl.handle.net/10174/31242engAlbuquerque, A.; M.R. Félix; M. Laranjo; R. Charneca; J.M. Martins (2020). Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds. In: V PhD Students Meeting in Environmental and Agriculture – Book of Abstracts, Pólo da Mitra, Valverde, Portugal, p. 17.ZOOandrealb@uevora.ptndndndjmartins@uevora.pt226Albuquerque, A.Félix, M.R.Laranjo, M.Charneca, R.Martins, J.M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:30:35Zoai:dspace.uevora.pt:10174/31242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:20:28.056868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
title Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
spellingShingle Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
Albuquerque, A.
Alentejano pig
Gene variability
DEGs
meat quality
Bísaro pig
lipid metabolism
title_short Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
title_full Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
title_fullStr Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
title_full_unstemmed Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
title_sort Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds
author Albuquerque, A.
author_facet Albuquerque, A.
Félix, M.R.
Laranjo, M.
Charneca, R.
Martins, J.M.
author_role author
author2 Félix, M.R.
Laranjo, M.
Charneca, R.
Martins, J.M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Albuquerque, A.
Félix, M.R.
Laranjo, M.
Charneca, R.
Martins, J.M.
dc.subject.por.fl_str_mv Alentejano pig
Gene variability
DEGs
meat quality
Bísaro pig
lipid metabolism
topic Alentejano pig
Gene variability
DEGs
meat quality
Bísaro pig
lipid metabolism
description The Alentejano (AL) and Bísaro (BI) pigs stand as the predominant local breeds raised in Portugal. AL pigs are characterized by lower growth rates and precocious and higher lipid deposition, particularly of monounsaturated fatty acids, when compared to BI pigs. Lipid and fatty acids composition of meat and meat products are important features for consumers health, and gene expression is a decisive process determining fatty acid composition and deposition rates in the muscle tissue of every mammal. This study investigates the transcriptomic fundamentals associated to the phenotypical differences through a real-time qPCR approach on a set of candidate genes responsible for regulating lipid metabolism processes. Longissimus lumborum samples were collected at slaughter from five animals of each breed raised under similar conditions until ~150kg BW. Total RNA was extracted, and target cDNA was amplified to estimate expression levels when compared to endogenous control genes. Several lipogenic related genes were found to be overexpressed in the AL breed including ACLY (log2FC=0.50, p<0.05), ELOVL6 (log2FC=0.67, p<0.01), ME1 (log2FC=0.78, p<0.05), while other major lipogenic markers, such as FASN (log2FC=0.45, p=0.09) and ACACA (log2FC=0.28, p=0.17) did not attain statistical significancy, but indicate a tendency towards AL. The gene coding for the central appetite regulation hormone (LEP) was detected as significantly overexpressed in AL (log2FC=1.60, p<0.05), which may suggest the occurrence of leptin resistance in this breed, comparable to what happens with the genetically similar Iberian pig, failing to decrease feeding and increase stored energy expenditure. Similarly, adiponectin resistance is predicted to have developed in AL pigs since higher ADIPOQ levels (log2FC=0.90, p<0.01) are associated with leaner animals due to the activation of fatty acid oxidation pathways by this regulator. These results agree with the previously described high intramuscular fat content profile of the AL breed, essential in enhancing the overall sensory quality of its meat and meat products.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01T00:00:00Z
2022-03-07T12:28:14Z
2022-03-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/31242
http://hdl.handle.net/10174/31242
url http://hdl.handle.net/10174/31242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Albuquerque, A.; M.R. Félix; M. Laranjo; R. Charneca; J.M. Martins (2020). Differentially expressed genes involved in lipid metabolism in the muscle tissue of Alentejano and Bísaro pig breeds. In: V PhD Students Meeting in Environmental and Agriculture – Book of Abstracts, Pólo da Mitra, Valverde, Portugal, p. 17.
ZOO
andrealb@uevora.pt
nd
nd
nd
jmartins@uevora.pt
226
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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