Biofilm formation among clinical and food isolates of Listeria monocytogenes

Detalhes bibliográficos
Autor(a) principal: Barbosa, Joana
Data de Publicação: 2013
Outros Autores: Borges, Sandra, Camilo, Ruth, Magalhães, Rui, Ferreira, Vânia, Santos, Isabel, Silva, Joana, Almeida, Gonçalo, Teixeira, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/14337
Resumo: Objective. A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h. Methods. Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions. Results. Significant differences ( ) between clinical and food isolates were observed. At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak. At 4°C during 5 days, 65 and 59% isolates, from food and clinical cases, respectively, were classified as weak. After both sublethal stresses, at 37°C just one of the five isolates tested was shown to be more sensitive to subsequent acidic exposure. However, at 4°C both stresses did not confer either sensitivity or resistance. Conclusions. Significant differences between isolates origin, temperature, and sublethal acidic stress were observed concerning the ability to form biofilms. Strain, origin, and environmental conditions can determine the level of biofilm production by L. monocytogenes isolates.
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spelling Biofilm formation among clinical and food isolates of Listeria monocytogenesObjective. A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h. Methods. Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions. Results. Significant differences ( ) between clinical and food isolates were observed. At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak. At 4°C during 5 days, 65 and 59% isolates, from food and clinical cases, respectively, were classified as weak. After both sublethal stresses, at 37°C just one of the five isolates tested was shown to be more sensitive to subsequent acidic exposure. However, at 4°C both stresses did not confer either sensitivity or resistance. Conclusions. Significant differences between isolates origin, temperature, and sublethal acidic stress were observed concerning the ability to form biofilms. Strain, origin, and environmental conditions can determine the level of biofilm production by L. monocytogenes isolates.Hindawi Publishing CorporationVeritati - Repositório Institucional da Universidade Católica PortuguesaBarbosa, JoanaBorges, SandraCamilo, RuthMagalhães, RuiFerreira, VâniaSantos, IsabelSilva, JoanaAlmeida, GonçaloTeixeira, Paula2014-05-08T15:07:43Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/14337engBARBOSA, Joana...[et al.] - Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology. ISSN 1687-9198. Vol. 2013 (2013), 6 p.1687-918X10.1155/2013/5249751687-9198info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:18:44Zoai:repositorio.ucp.pt:10400.14/14337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:11:23.262442Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilm formation among clinical and food isolates of Listeria monocytogenes
title Biofilm formation among clinical and food isolates of Listeria monocytogenes
spellingShingle Biofilm formation among clinical and food isolates of Listeria monocytogenes
Barbosa, Joana
title_short Biofilm formation among clinical and food isolates of Listeria monocytogenes
title_full Biofilm formation among clinical and food isolates of Listeria monocytogenes
title_fullStr Biofilm formation among clinical and food isolates of Listeria monocytogenes
title_full_unstemmed Biofilm formation among clinical and food isolates of Listeria monocytogenes
title_sort Biofilm formation among clinical and food isolates of Listeria monocytogenes
author Barbosa, Joana
author_facet Barbosa, Joana
Borges, Sandra
Camilo, Ruth
Magalhães, Rui
Ferreira, Vânia
Santos, Isabel
Silva, Joana
Almeida, Gonçalo
Teixeira, Paula
author_role author
author2 Borges, Sandra
Camilo, Ruth
Magalhães, Rui
Ferreira, Vânia
Santos, Isabel
Silva, Joana
Almeida, Gonçalo
Teixeira, Paula
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Barbosa, Joana
Borges, Sandra
Camilo, Ruth
Magalhães, Rui
Ferreira, Vânia
Santos, Isabel
Silva, Joana
Almeida, Gonçalo
Teixeira, Paula
description Objective. A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h. Methods. Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions. Results. Significant differences ( ) between clinical and food isolates were observed. At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak. At 4°C during 5 days, 65 and 59% isolates, from food and clinical cases, respectively, were classified as weak. After both sublethal stresses, at 37°C just one of the five isolates tested was shown to be more sensitive to subsequent acidic exposure. However, at 4°C both stresses did not confer either sensitivity or resistance. Conclusions. Significant differences between isolates origin, temperature, and sublethal acidic stress were observed concerning the ability to form biofilms. Strain, origin, and environmental conditions can determine the level of biofilm production by L. monocytogenes isolates.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-05-08T15:07:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/14337
url http://hdl.handle.net/10400.14/14337
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BARBOSA, Joana...[et al.] - Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. International Journal of Microbiology. ISSN 1687-9198. Vol. 2013 (2013), 6 p.
1687-918X
10.1155/2013/524975
1687-9198
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
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