Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/7298 |
Resumo: | Review |
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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and resultsDNA DamageComet AssayMinimum Information for Reporting Comet Assay (MIRCA)RecommendationsReviewThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Nature ResearchRepositório Científico do Instituto Nacional de SaúdeMøller, PeterAzqueta, AmayaBoutet-Robinet, ElisaKoppen, GudrunBonassi, StefanoMilić, MirtaGajski, GoranCosta, SolangeTeixeira, João PauloCosta Pereira, CristianaDusinska, MariaGodschalk, RogerBrunborg, GunnarGutzkow, Kristine B.Giovannelli, LisaCooke, Marcus S.Richling, ElkeLaffon, BlancaValdiglesias, VanessaBasaran, NursenDel Bo’, CristianZegura, BojanaNovak, MatjazStopper, HelgaVodicka, PavelVodenkova, Sonade Andrade, Vanessa MoraesSramkova, MonikaGabelova, AlenaCollins, AndrewLangie, Sabine A.S.2021-03-03T16:34:36Z2020-122020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7298engNat Protoc. 2020 Dec;15(12):3817-3826. doi: 10.1038/s41596-020-0398-1. Epub 2020 Oct 26.1754-218910.1038/s41596-020-0398-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:01Zoai:repositorio.insa.pt:10400.18/7298Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:06.795055Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| title |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| spellingShingle |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results Møller, Peter DNA Damage Comet Assay Minimum Information for Reporting Comet Assay (MIRCA) Recommendations |
| title_short |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| title_full |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| title_fullStr |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| title_full_unstemmed |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| title_sort |
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results |
| author |
Møller, Peter |
| author_facet |
Møller, Peter Azqueta, Amaya Boutet-Robinet, Elisa Koppen, Gudrun Bonassi, Stefano Milić, Mirta Gajski, Goran Costa, Solange Teixeira, João Paulo Costa Pereira, Cristiana Dusinska, Maria Godschalk, Roger Brunborg, Gunnar Gutzkow, Kristine B. Giovannelli, Lisa Cooke, Marcus S. Richling, Elke Laffon, Blanca Valdiglesias, Vanessa Basaran, Nursen Del Bo’, Cristian Zegura, Bojana Novak, Matjaz Stopper, Helga Vodicka, Pavel Vodenkova, Sona de Andrade, Vanessa Moraes Sramkova, Monika Gabelova, Alena Collins, Andrew Langie, Sabine A.S. |
| author_role |
author |
| author2 |
Azqueta, Amaya Boutet-Robinet, Elisa Koppen, Gudrun Bonassi, Stefano Milić, Mirta Gajski, Goran Costa, Solange Teixeira, João Paulo Costa Pereira, Cristiana Dusinska, Maria Godschalk, Roger Brunborg, Gunnar Gutzkow, Kristine B. Giovannelli, Lisa Cooke, Marcus S. Richling, Elke Laffon, Blanca Valdiglesias, Vanessa Basaran, Nursen Del Bo’, Cristian Zegura, Bojana Novak, Matjaz Stopper, Helga Vodicka, Pavel Vodenkova, Sona de Andrade, Vanessa Moraes Sramkova, Monika Gabelova, Alena Collins, Andrew Langie, Sabine A.S. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Møller, Peter Azqueta, Amaya Boutet-Robinet, Elisa Koppen, Gudrun Bonassi, Stefano Milić, Mirta Gajski, Goran Costa, Solange Teixeira, João Paulo Costa Pereira, Cristiana Dusinska, Maria Godschalk, Roger Brunborg, Gunnar Gutzkow, Kristine B. Giovannelli, Lisa Cooke, Marcus S. Richling, Elke Laffon, Blanca Valdiglesias, Vanessa Basaran, Nursen Del Bo’, Cristian Zegura, Bojana Novak, Matjaz Stopper, Helga Vodicka, Pavel Vodenkova, Sona de Andrade, Vanessa Moraes Sramkova, Monika Gabelova, Alena Collins, Andrew Langie, Sabine A.S. |
| dc.subject.por.fl_str_mv |
DNA Damage Comet Assay Minimum Information for Reporting Comet Assay (MIRCA) Recommendations |
| topic |
DNA Damage Comet Assay Minimum Information for Reporting Comet Assay (MIRCA) Recommendations |
| description |
Review |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12 2020-12-01T00:00:00Z 2021-03-03T16:34:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7298 |
| url |
http://hdl.handle.net/10400.18/7298 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Nat Protoc. 2020 Dec;15(12):3817-3826. doi: 10.1038/s41596-020-0398-1. Epub 2020 Oct 26. 1754-2189 10.1038/s41596-020-0398-1 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Nature Research |
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Nature Research |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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