Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/59092 |
Resumo: | One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy. |
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Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactorGlucanGlucoseBiofuelsAutohydrolysisSeverity FactorBiorefineryScience & TechnologyOne of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.This project was funded by the Secretary of Public Education of Mexico - Mexican Science and Technology Council (SEP-CONACYT) with the Basic Science Project-2015-01 (Ref. 254808). Marcela Sofía Pino also thanks the National Council for Science and Technology (CONACYT, Mexico) for her Master Fellowship support (grant number: 611312/452636), and Dr. Michele Michelin thanks the Portuguese Foundation for Science and Technology (FCT) for her postdoctoral fellowship (SFRH/BPD/100786/2014).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPino, Marcela SofíaRodríguez-Jasso, Rosa M.Michelin, MicheleRuiz, Héctor A.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59092engPino, Marcela; Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A., Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor. Carbohydrate Polymers, 211, 349-359, 20190144-86170144-861710.1016/j.carbpol.2019.01.11130824099http://www.journals.elsevier.com/carbohydrate-polymers/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:19:43Zoai:repositorium.sdum.uminho.pt:1822/59092Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:12:41.365967Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
title |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
spellingShingle |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor Pino, Marcela Sofía Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery Science & Technology |
title_short |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
title_full |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
title_fullStr |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
title_full_unstemmed |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
title_sort |
Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
author |
Pino, Marcela Sofía |
author_facet |
Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
author_role |
author |
author2 |
Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
dc.subject.por.fl_str_mv |
Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery Science & Technology |
topic |
Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery Science & Technology |
description |
One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/59092 |
url |
http://hdl.handle.net/1822/59092 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pino, Marcela; Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A., Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor. Carbohydrate Polymers, 211, 349-359, 2019 0144-8617 0144-8617 10.1016/j.carbpol.2019.01.111 30824099 http://www.journals.elsevier.com/carbohydrate-polymers/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132563344719872 |