The hydrophobic polysaccharides of apple pomace

Detalhes bibliográficos
Autor(a) principal: Fernandes, Pedro A. R.
Data de Publicação: 2019
Outros Autores: Silva, Artur M. S., Evtuguin, Dmitry V., Nunes, Fernando M., Wessel, Dulcineia F., Cardoso, Susana M., Coimbra, Manuel A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37552
Resumo: In this work, polysaccharides extracted with hot water from apple pomace were isolated by C18 cartridge solid-phase extraction at pH 7 (Fr7). Dialysis (12-14 kDa) of this fraction allowed to obtain 17% (w/w) of polymeric material composed by 65% of polysaccharides, mainly arabinose (58 mol%), galacturonic acid (16 mol%) and glucose (10 mol%). Folin-Ciocalteu assay showed 62 g of phloridzin equiv/kg of polyphenols. Moreover, adjusting to pH 3, it was possible to retain an additional fraction (Fr3) representing a further 4% of the polymeric material. Fr3 contained 53% of polysaccharides composed mainly by galacturonic acid (66 mol%) and polyphenols accounted for 37 g of phloridzin equiv/kg. Precipitation with ethanol and subsequent methylation and NMR spectroscopic analysis of Fr7 dialysate allowed the identification of covalently-linked pectic-polyphenol-xyloglucan and arabinan-polyphenol complexes. These structures are possibly formed as a result of polyphenol oxidation reactions during the industrial processing of apples, conferring hydrophobic characteristics to apple pomace polysaccharides.
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spelling The hydrophobic polysaccharides of apple pomacePectic polysaccharidesXyloglucanArabinanPolyphenol interactionNMRIn this work, polysaccharides extracted with hot water from apple pomace were isolated by C18 cartridge solid-phase extraction at pH 7 (Fr7). Dialysis (12-14 kDa) of this fraction allowed to obtain 17% (w/w) of polymeric material composed by 65% of polysaccharides, mainly arabinose (58 mol%), galacturonic acid (16 mol%) and glucose (10 mol%). Folin-Ciocalteu assay showed 62 g of phloridzin equiv/kg of polyphenols. Moreover, adjusting to pH 3, it was possible to retain an additional fraction (Fr3) representing a further 4% of the polymeric material. Fr3 contained 53% of polysaccharides composed mainly by galacturonic acid (66 mol%) and polyphenols accounted for 37 g of phloridzin equiv/kg. Precipitation with ethanol and subsequent methylation and NMR spectroscopic analysis of Fr7 dialysate allowed the identification of covalently-linked pectic-polyphenol-xyloglucan and arabinan-polyphenol complexes. These structures are possibly formed as a result of polyphenol oxidation reactions during the industrial processing of apples, conferring hydrophobic characteristics to apple pomace polysaccharides.Elsevier2023-05-05T15:13:08Z2019-11-01T00:00:00Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37552eng0144-861710.1016/j.carbpol.2019.115132Fernandes, Pedro A. R.Silva, Artur M. S.Evtuguin, Dmitry V.Nunes, Fernando M.Wessel, Dulcineia F.Cardoso, Susana M.Coimbra, Manuel A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-06T04:45:45Zoai:ria.ua.pt:10773/37552Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-06T04:45:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The hydrophobic polysaccharides of apple pomace
title The hydrophobic polysaccharides of apple pomace
spellingShingle The hydrophobic polysaccharides of apple pomace
Fernandes, Pedro A. R.
Pectic polysaccharides
Xyloglucan
Arabinan
Polyphenol interaction
NMR
title_short The hydrophobic polysaccharides of apple pomace
title_full The hydrophobic polysaccharides of apple pomace
title_fullStr The hydrophobic polysaccharides of apple pomace
title_full_unstemmed The hydrophobic polysaccharides of apple pomace
title_sort The hydrophobic polysaccharides of apple pomace
author Fernandes, Pedro A. R.
author_facet Fernandes, Pedro A. R.
Silva, Artur M. S.
Evtuguin, Dmitry V.
Nunes, Fernando M.
Wessel, Dulcineia F.
Cardoso, Susana M.
Coimbra, Manuel A.
author_role author
author2 Silva, Artur M. S.
Evtuguin, Dmitry V.
Nunes, Fernando M.
Wessel, Dulcineia F.
Cardoso, Susana M.
Coimbra, Manuel A.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Pedro A. R.
Silva, Artur M. S.
Evtuguin, Dmitry V.
Nunes, Fernando M.
Wessel, Dulcineia F.
Cardoso, Susana M.
Coimbra, Manuel A.
dc.subject.por.fl_str_mv Pectic polysaccharides
Xyloglucan
Arabinan
Polyphenol interaction
NMR
topic Pectic polysaccharides
Xyloglucan
Arabinan
Polyphenol interaction
NMR
description In this work, polysaccharides extracted with hot water from apple pomace were isolated by C18 cartridge solid-phase extraction at pH 7 (Fr7). Dialysis (12-14 kDa) of this fraction allowed to obtain 17% (w/w) of polymeric material composed by 65% of polysaccharides, mainly arabinose (58 mol%), galacturonic acid (16 mol%) and glucose (10 mol%). Folin-Ciocalteu assay showed 62 g of phloridzin equiv/kg of polyphenols. Moreover, adjusting to pH 3, it was possible to retain an additional fraction (Fr3) representing a further 4% of the polymeric material. Fr3 contained 53% of polysaccharides composed mainly by galacturonic acid (66 mol%) and polyphenols accounted for 37 g of phloridzin equiv/kg. Precipitation with ethanol and subsequent methylation and NMR spectroscopic analysis of Fr7 dialysate allowed the identification of covalently-linked pectic-polyphenol-xyloglucan and arabinan-polyphenol complexes. These structures are possibly formed as a result of polyphenol oxidation reactions during the industrial processing of apples, conferring hydrophobic characteristics to apple pomace polysaccharides.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-01T00:00:00Z
2019-11-01
2023-05-05T15:13:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37552
url http://hdl.handle.net/10773/37552
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8617
10.1016/j.carbpol.2019.115132
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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