Fast NMR method to probe solvent accessibility and disordered regions in proteins

Detalhes bibliográficos
Autor(a) principal: Faustino, André F.
Data de Publicação: 2019
Outros Autores: Barbosa, Glauce M., Silva, Micael, Castanho, Miguel, Da Poian, Andrea T ., Cabrita, Eurico J., Santos, Nuno C., Almeida, Fabio C. L., Martins, Ivo C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/37911
Resumo: © Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling Fast NMR method to probe solvent accessibility and disordered regions in proteins© Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Understanding protein structure and dynamics, which govern key cellular processes, is crucial for basic and applied research. Intrinsically disordered protein (IDP) regions display multifunctionality via alternative transient conformations, being key players in disease mechanisms. IDP regions are abundant, namely in small viruses, allowing a large number of functions out of a small proteome. The relation between protein function and structure is thus now seen from a different perspective: as IDP regions enable transient structural arrangements, each conformer can play different roles within the cell. However, as IDP regions are hard and time-consuming to study via classical techniques (optimized for globular proteins with unique conformations), new methods are required. Here, employing the dengue virus (DENV) capsid (C) protein and the immunoglobulin-binding domain of streptococcal protein G, we describe a straightforward NMR method to differentiate the solvent accessibility of single amino acid N-H groups in structured and IDP regions. We also gain insights into DENV C flexible fold region biological activity. The method, based on minimal pH changes, uses the well-established 1H-15N HSQC pulse sequence and is easily implementable in current protein NMR routines. The data generated are simple to interpret, with this rapid approach being an useful first-choice IDPs characterization method.This work was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) projects PTDC/QUI-BIQ/112929/2009, PTDC/SAU-ENB/117013/2010 and PTDC/BBB-BQB/3494/2014, Calouste Gulbenkian Foundation (FCG, Portugal) project Science Frontiers Research Prize 2010, European Union Marie Skłodowska-Curie Research and Innovation Staff Exchange H2020-MSCA-RISE-2014 project INPACT (Grant 644167), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil, grant numbers 471239/2012-7 and 306669/2013-7) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil, grant numbers E-26/110.636/2012, E-26/110.092/2013 and E-26/201.167/2014). AFF acknowledges FCT-MCTES fellowship SFRH/BD/77609/2011. MS acknowledges FCT-MCTES fellowship PD/BD/128202/2016. ICM acknowledges consecutive funding from the FCT-MCTES fellowship SFRH/BPD/74287/2010 and the Program “Investigador FCT” (IF/00772/2013 Research Contract). This work was also supported by LISBOA01-0145-FEDER-007391 project, cofounded by FEDER, through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020, and Fundação para a Ciência e a Tecnologia. The NMR spectrometers at FCT NOVA are part of Rede Nacional de RMN (PTNMR), supported by FCT-MCTES (ROTEIRO/0031/2013 - PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC.Nature ResearchRepositório da Universidade de LisboaFaustino, André F.Barbosa, Glauce M.Silva, MicaelCastanho, MiguelDa Poian, Andrea T .Cabrita, Eurico J.Santos, Nuno C.Almeida, Fabio C. L.Martins, Ivo C.2019-04-12T15:31:26Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/37911engScientific Reports (2019) 9:16472045-232210.1038/s41598-018-37599-zinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:35:22Zoai:repositorio.ul.pt:10451/37911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:51:52.726837Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fast NMR method to probe solvent accessibility and disordered regions in proteins
title Fast NMR method to probe solvent accessibility and disordered regions in proteins
spellingShingle Fast NMR method to probe solvent accessibility and disordered regions in proteins
Faustino, André F.
title_short Fast NMR method to probe solvent accessibility and disordered regions in proteins
title_full Fast NMR method to probe solvent accessibility and disordered regions in proteins
title_fullStr Fast NMR method to probe solvent accessibility and disordered regions in proteins
title_full_unstemmed Fast NMR method to probe solvent accessibility and disordered regions in proteins
title_sort Fast NMR method to probe solvent accessibility and disordered regions in proteins
author Faustino, André F.
author_facet Faustino, André F.
Barbosa, Glauce M.
Silva, Micael
Castanho, Miguel
Da Poian, Andrea T .
Cabrita, Eurico J.
Santos, Nuno C.
Almeida, Fabio C. L.
Martins, Ivo C.
author_role author
author2 Barbosa, Glauce M.
Silva, Micael
Castanho, Miguel
Da Poian, Andrea T .
Cabrita, Eurico J.
Santos, Nuno C.
Almeida, Fabio C. L.
Martins, Ivo C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Faustino, André F.
Barbosa, Glauce M.
Silva, Micael
Castanho, Miguel
Da Poian, Andrea T .
Cabrita, Eurico J.
Santos, Nuno C.
Almeida, Fabio C. L.
Martins, Ivo C.
description © Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-12T15:31:26Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports (2019) 9:1647
2045-2322
10.1038/s41598-018-37599-z
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