Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1861 |
Resumo: | The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C12-18EO5. |
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Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilizationScience & TechnologyThe aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C12-18EO5.Oy Panimolaboratorio-Bryggerilaboratorium Ab.Finland. Technology Development Centre (Tekes).American Society for Microbiology (ASM)Universidade do MinhoNakari-Setälä, T.Azeredo, JoanaHenriques, MarianaOliveira, RosárioTeixeira, J. A.Linder, M.Penttilä, M.2002-072002-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1861eng“Applied and environmental microbiology”. ISSN 0099-2240. 68:7 (2002) 3385-3391.0099-224010.1128/AEM.68.7.3385-3391.2002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:34Zoai:repositorium.sdum.uminho.pt:1822/1861Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:42.776909Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
title |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
spellingShingle |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization Nakari-Setälä, T. Science & Technology |
title_short |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
title_full |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
title_fullStr |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
title_full_unstemmed |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
title_sort |
Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization |
author |
Nakari-Setälä, T. |
author_facet |
Nakari-Setälä, T. Azeredo, Joana Henriques, Mariana Oliveira, Rosário Teixeira, J. A. Linder, M. Penttilä, M. |
author_role |
author |
author2 |
Azeredo, Joana Henriques, Mariana Oliveira, Rosário Teixeira, J. A. Linder, M. Penttilä, M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Nakari-Setälä, T. Azeredo, Joana Henriques, Mariana Oliveira, Rosário Teixeira, J. A. Linder, M. Penttilä, M. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C12-18EO5. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-07 2002-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1861 |
url |
http://hdl.handle.net/1822/1861 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
“Applied and environmental microbiology”. ISSN 0099-2240. 68:7 (2002) 3385-3391. 0099-2240 10.1128/AEM.68.7.3385-3391.2002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132469809643520 |