Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/78963 |
Resumo: | Recurrent microbial infections are a major cause of surgical failure and morbidity. Wound healing strategies based on hydrogels have been proposed to provide at once a barrier against pathogen microbial colonization, as well as a favorable environment for tissue repair. Nevertheless, most biocompatible hydrogel materials are more bacteriostatic than antimicrobial materials, and lack specific action against pathogens. Silver-loaded polymeric nanocomposites have efficient and selective activity against pathogenic organisms exploitable for wound healing. However, the loading of metallic nanostructures into hydrogels represents a major challenge due to the low stability of metal colloids in aqueous environments. In this context, the aim of the present study was the development of highly stable silver nanoparticles (AgNPs) as novel potential antimicrobial agents for hyaluronic acids hydrogels. Two candidate stabilizing agents obtained from natural and renewable sources, namely cellulose nanocrystals and ulvan polysaccharide, were exploited to ensure high stability of the silver colloid. Both stabilizing agents possess inherent bioactivity and biocompatibility, as well as the ability to stabilize metal nanostructures thanks to their supramolecular structures. Silver nitrate reduction through sodium borohydride in presence of the selected stabilizing agents was adopted as a model strategy to achieve AgNPs with narrow size distribution. Optimized AgNPs stabilized with the two investigated polysaccharides demonstrated high stability in phosphate buffer saline solution and strong antimicrobial activity. Loading of the developed AgNPs into photocrosslinked methacrylated hyaluronic acid hydrogels was also investigated for the first time as an effective strategy to develop novel antimicrobial wound dressing materials. |
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Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogelsNanotechnologySilver nanoparticlesHydrogelsPolysaccharidesUlvanCellulose nanocrystalsWound healingScience & TechnologyRecurrent microbial infections are a major cause of surgical failure and morbidity. Wound healing strategies based on hydrogels have been proposed to provide at once a barrier against pathogen microbial colonization, as well as a favorable environment for tissue repair. Nevertheless, most biocompatible hydrogel materials are more bacteriostatic than antimicrobial materials, and lack specific action against pathogens. Silver-loaded polymeric nanocomposites have efficient and selective activity against pathogenic organisms exploitable for wound healing. However, the loading of metallic nanostructures into hydrogels represents a major challenge due to the low stability of metal colloids in aqueous environments. In this context, the aim of the present study was the development of highly stable silver nanoparticles (AgNPs) as novel potential antimicrobial agents for hyaluronic acids hydrogels. Two candidate stabilizing agents obtained from natural and renewable sources, namely cellulose nanocrystals and ulvan polysaccharide, were exploited to ensure high stability of the silver colloid. Both stabilizing agents possess inherent bioactivity and biocompatibility, as well as the ability to stabilize metal nanostructures thanks to their supramolecular structures. Silver nitrate reduction through sodium borohydride in presence of the selected stabilizing agents was adopted as a model strategy to achieve AgNPs with narrow size distribution. Optimized AgNPs stabilized with the two investigated polysaccharides demonstrated high stability in phosphate buffer saline solution and strong antimicrobial activity. Loading of the developed AgNPs into photocrosslinked methacrylated hyaluronic acid hydrogels was also investigated for the first time as an effective strategy to develop novel antimicrobial wound dressing materials.This research was supported by the Portuguese Foundation for Science and Technology (FCT) under the projects PTDC/BII-BIO/28870/2017 and POCI-01-0145-FEDER-007038 (UID/Multi/ 50026/2013), and by the European Regional Development Fund (FEDER) through the “COMPETE”— Operational Program for Competitiveness factors (FCOMP-01-0124-FEDER-028120). A.R.F. thanks FCT through the Post-Doctoral scholarship SFRH/BPD/100760/2014.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoMassironi, AlessioFranco, Albina RibeiroBabo, Pedro Miguel SousaPuppi, DarioChiellini, FedericaReis, R. L.Gomes, Manuela E.2022-02-152022-02-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/78963engMassironi, A.; Franco, A.R.; Babo, P.S.; Puppi, D.; Chiellini, F.; Reis, R.L.; Gomes, M.E. Development and Characterization of Highly Stable Silver NanoParticles as Novel Potential Antimicrobial Agents for Wound Healing Hydrogels. Int. J. Mol. Sci. 2022, 23, 2161. https://doi.org/10.3390/ijms230421611661-65961422-006710.3390/ijms23042161352162772161https://www.mdpi.com/1422-0067/23/4/2161info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:58Zoai:repositorium.sdum.uminho.pt:1822/78963Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:02.728358Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
title |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
spellingShingle |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels Massironi, Alessio Nanotechnology Silver nanoparticles Hydrogels Polysaccharides Ulvan Cellulose nanocrystals Wound healing Science & Technology |
title_short |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
title_full |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
title_fullStr |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
title_full_unstemmed |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
title_sort |
Development and characterization of highly stable silver nanoparticles as novel potential antimicrobial agents for wound healing hydrogels |
author |
Massironi, Alessio |
author_facet |
Massironi, Alessio Franco, Albina Ribeiro Babo, Pedro Miguel Sousa Puppi, Dario Chiellini, Federica Reis, R. L. Gomes, Manuela E. |
author_role |
author |
author2 |
Franco, Albina Ribeiro Babo, Pedro Miguel Sousa Puppi, Dario Chiellini, Federica Reis, R. L. Gomes, Manuela E. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Massironi, Alessio Franco, Albina Ribeiro Babo, Pedro Miguel Sousa Puppi, Dario Chiellini, Federica Reis, R. L. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Nanotechnology Silver nanoparticles Hydrogels Polysaccharides Ulvan Cellulose nanocrystals Wound healing Science & Technology |
topic |
Nanotechnology Silver nanoparticles Hydrogels Polysaccharides Ulvan Cellulose nanocrystals Wound healing Science & Technology |
description |
Recurrent microbial infections are a major cause of surgical failure and morbidity. Wound healing strategies based on hydrogels have been proposed to provide at once a barrier against pathogen microbial colonization, as well as a favorable environment for tissue repair. Nevertheless, most biocompatible hydrogel materials are more bacteriostatic than antimicrobial materials, and lack specific action against pathogens. Silver-loaded polymeric nanocomposites have efficient and selective activity against pathogenic organisms exploitable for wound healing. However, the loading of metallic nanostructures into hydrogels represents a major challenge due to the low stability of metal colloids in aqueous environments. In this context, the aim of the present study was the development of highly stable silver nanoparticles (AgNPs) as novel potential antimicrobial agents for hyaluronic acids hydrogels. Two candidate stabilizing agents obtained from natural and renewable sources, namely cellulose nanocrystals and ulvan polysaccharide, were exploited to ensure high stability of the silver colloid. Both stabilizing agents possess inherent bioactivity and biocompatibility, as well as the ability to stabilize metal nanostructures thanks to their supramolecular structures. Silver nitrate reduction through sodium borohydride in presence of the selected stabilizing agents was adopted as a model strategy to achieve AgNPs with narrow size distribution. Optimized AgNPs stabilized with the two investigated polysaccharides demonstrated high stability in phosphate buffer saline solution and strong antimicrobial activity. Loading of the developed AgNPs into photocrosslinked methacrylated hyaluronic acid hydrogels was also investigated for the first time as an effective strategy to develop novel antimicrobial wound dressing materials. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-15 2022-02-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/78963 |
url |
https://hdl.handle.net/1822/78963 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Massironi, A.; Franco, A.R.; Babo, P.S.; Puppi, D.; Chiellini, F.; Reis, R.L.; Gomes, M.E. Development and Characterization of Highly Stable Silver NanoParticles as Novel Potential Antimicrobial Agents for Wound Healing Hydrogels. Int. J. Mol. Sci. 2022, 23, 2161. https://doi.org/10.3390/ijms23042161 1661-6596 1422-0067 10.3390/ijms23042161 35216277 2161 https://www.mdpi.com/1422-0067/23/4/2161 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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