Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/3889 |
Resumo: | ABSTRACT: In this study, Rhodosporidium toruloides and Tetradesmus obliquus were used for lipid and carotenoid production in mixed cultures using primary brewery wastewater (PBWW) as a culture medium, supplemented with sugarcane molasses (SCM) as a carbon source and urea as a nitrogen source. To improve biomass, lipid, and carotenoid production by R. toruloides and T obliquus mixed cultures, initial SCM concentrations ranging from 10 to 280 g L-1 were tested. The medium that allowed higher lipid content (26.2% w/w dry cell weight (DCW)) and higher carotenoid productivity (10.47 mu g L-1 h(-1)) was the PBWW medium supplemented with 100 g L-1 of SCM and 2 g L-1 of urea, which was further used in the fed-batch mixed cultivation performed in a 7-L bioreactor. A maximum biomass concentration of 58.6 g L-1 and maximum lipid content of 31.2% w/w DCW were obtained in the fed-batch cultivation. PBWW supplemented with SCM was successfully used as a low-cost medium to produce lipids and carotenoids in a R. toruloides and T obliquus mixed culture, with higher productivities than in pure cultures, which can significantly reduce the cost of the biofuels obtained. |
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Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and ureaMicroalgaeRhodosporidium toruloidesTetradesmus obliquusTetradesmus obliquusLipidsBrewery effluentCarotenoidsABSTRACT: In this study, Rhodosporidium toruloides and Tetradesmus obliquus were used for lipid and carotenoid production in mixed cultures using primary brewery wastewater (PBWW) as a culture medium, supplemented with sugarcane molasses (SCM) as a carbon source and urea as a nitrogen source. To improve biomass, lipid, and carotenoid production by R. toruloides and T obliquus mixed cultures, initial SCM concentrations ranging from 10 to 280 g L-1 were tested. The medium that allowed higher lipid content (26.2% w/w dry cell weight (DCW)) and higher carotenoid productivity (10.47 mu g L-1 h(-1)) was the PBWW medium supplemented with 100 g L-1 of SCM and 2 g L-1 of urea, which was further used in the fed-batch mixed cultivation performed in a 7-L bioreactor. A maximum biomass concentration of 58.6 g L-1 and maximum lipid content of 31.2% w/w DCW were obtained in the fed-batch cultivation. PBWW supplemented with SCM was successfully used as a low-cost medium to produce lipids and carotenoids in a R. toruloides and T obliquus mixed culture, with higher productivities than in pure cultures, which can significantly reduce the cost of the biofuels obtained.SpringerRepositório do LNEGDias, CarlaNobre, B. P.Santos, J. A. L.Reis, AlbertoSilva, Teresa Lopes da2022-07-28T10:06:05Z2022-072022-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3889engDias, Carla... [et.al.] - Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea. In: Applied Biochemistry and Biotechnology, 2022, Early Access0273-228910.1007/s12010-022-04034-z1559-0291info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-02-12T05:16:25Zoai:repositorio.lneg.pt:10400.9/3889Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:37:08.818268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
title |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
spellingShingle |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea Dias, Carla Microalgae Rhodosporidium toruloides Tetradesmus obliquus Tetradesmus obliquus Lipids Brewery effluent Carotenoids |
title_short |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
title_full |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
title_fullStr |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
title_full_unstemmed |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
title_sort |
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea |
author |
Dias, Carla |
author_facet |
Dias, Carla Nobre, B. P. Santos, J. A. L. Reis, Alberto Silva, Teresa Lopes da |
author_role |
author |
author2 |
Nobre, B. P. Santos, J. A. L. Reis, Alberto Silva, Teresa Lopes da |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Dias, Carla Nobre, B. P. Santos, J. A. L. Reis, Alberto Silva, Teresa Lopes da |
dc.subject.por.fl_str_mv |
Microalgae Rhodosporidium toruloides Tetradesmus obliquus Tetradesmus obliquus Lipids Brewery effluent Carotenoids |
topic |
Microalgae Rhodosporidium toruloides Tetradesmus obliquus Tetradesmus obliquus Lipids Brewery effluent Carotenoids |
description |
ABSTRACT: In this study, Rhodosporidium toruloides and Tetradesmus obliquus were used for lipid and carotenoid production in mixed cultures using primary brewery wastewater (PBWW) as a culture medium, supplemented with sugarcane molasses (SCM) as a carbon source and urea as a nitrogen source. To improve biomass, lipid, and carotenoid production by R. toruloides and T obliquus mixed cultures, initial SCM concentrations ranging from 10 to 280 g L-1 were tested. The medium that allowed higher lipid content (26.2% w/w dry cell weight (DCW)) and higher carotenoid productivity (10.47 mu g L-1 h(-1)) was the PBWW medium supplemented with 100 g L-1 of SCM and 2 g L-1 of urea, which was further used in the fed-batch mixed cultivation performed in a 7-L bioreactor. A maximum biomass concentration of 58.6 g L-1 and maximum lipid content of 31.2% w/w DCW were obtained in the fed-batch cultivation. PBWW supplemented with SCM was successfully used as a low-cost medium to produce lipids and carotenoids in a R. toruloides and T obliquus mixed culture, with higher productivities than in pure cultures, which can significantly reduce the cost of the biofuels obtained. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-28T10:06:05Z 2022-07 2022-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/3889 |
url |
http://hdl.handle.net/10400.9/3889 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dias, Carla... [et.al.] - Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus obliquus mixed culture using primary brewery wastewater supplemented with sugarcane molasses and urea. In: Applied Biochemistry and Biotechnology, 2022, Early Access 0273-2289 10.1007/s12010-022-04034-z 1559-0291 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130237691232256 |