Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents

Detalhes bibliográficos
Autor(a) principal: Tiago, Francisco José Martins
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/133430
Resumo: The increasing demand for medical cannabis urges the development for new and effective methods for the extraction of phytocannabinoids. Deep eutectic Solvents (DESs) are an alternative to the use of hazardous organic solvents typically used in the industry. In this study, DESs were developed, based on terpenes, sugars, and natural organic acids, as a green extraction media for the extraction of cannabis bioactive compounds. The factors influencing the extraction of bioactive components, such as the type of DESs and extraction time were investigated. Initial screening in hemp showed that the DES composed of Menthol: Lauric acid (2:1 M ratio) had a greater extraction efficiency of CBD and CBDA (11,07 ± 0,37 mg/g) of all the tested DESs and higher than ethanol. In medical cannabis Men:Lac (2:1 M ratio) allowed an extraction as effective as ethanol. Besides having a higher or equivalent extraction yield as the organic solvents tested, DESs showed to be more selective extracting less impurities, such as chlorophyll and waxes. These results coupled with the non-toxic, biodegradable, low cost and environmentally friendly characteristics of DESs, provided strong evidence that DESs represents a better alternative to organic solvents.
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spelling Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solventsCannabis sativaGreen ExtractionDeep Eutectic SolventsCannabinoidsDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaThe increasing demand for medical cannabis urges the development for new and effective methods for the extraction of phytocannabinoids. Deep eutectic Solvents (DESs) are an alternative to the use of hazardous organic solvents typically used in the industry. In this study, DESs were developed, based on terpenes, sugars, and natural organic acids, as a green extraction media for the extraction of cannabis bioactive compounds. The factors influencing the extraction of bioactive components, such as the type of DESs and extraction time were investigated. Initial screening in hemp showed that the DES composed of Menthol: Lauric acid (2:1 M ratio) had a greater extraction efficiency of CBD and CBDA (11,07 ± 0,37 mg/g) of all the tested DESs and higher than ethanol. In medical cannabis Men:Lac (2:1 M ratio) allowed an extraction as effective as ethanol. Besides having a higher or equivalent extraction yield as the organic solvents tested, DESs showed to be more selective extracting less impurities, such as chlorophyll and waxes. These results coupled with the non-toxic, biodegradable, low cost and environmentally friendly characteristics of DESs, provided strong evidence that DESs represents a better alternative to organic solvents.O aumento da procura por cannabis medicinal aumenta a procura de métodos novos e eficazes para a extração de fitocanabinóides. Os solventes eutéticos profundos (deep eutectic solvents, DESs) são uma alternativa ao uso de solventes orgânicos normalmente nocivos e tóxicos, usados na indústria. Neste estudo, vários DESs foram desenvolvidos, com base em terpenos, açúcares e ácidos orgânicos naturais, como um meio de extração verde para a obtenção de compostos bioativos a partir de Cannabis. Os fatores que influenciam a extração de compostos bioactivos, tais como o tipo de DES ou o tempo de extração foram avaliados. A triagem inicial realizada com cânhamo mostrou que os DES compostos por Mentol: ácido láurico (na razão molar de 2:1) tiveram uma maior eficiência de extração de CBD e CBDA (11,07 ± 0,37 mg / g) entre todos os DESs testados, sendo inclusivamente superior ao etanol. Na cannabis medicinal, Men:Lac (proporção de 2: 1 M) permitiu uma extração tão eficaz quanto o etanol. Além de apresentar um rendimento de extração superior ou equivalente ao dos solventes orgânicos testados, os DESs mostraram-se mais seletivos, extraindo menos impurezas, como clorofila e ceras. Esses resultados, juntamente com as características não tóxicas, biodegradáveis, de baixo custo e ambientalmente sustentáveis dos DESs, forneceram fortes evidências de que os DESs representam uma melhor alternativa aos solventes orgânicos.Duarte, AnaMatias, AnaRUNTiago, Francisco José Martins2024-11-01T01:32:12Z2022-02-172022-02-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/133430enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-11T01:37:50Zoai:run.unl.pt:10362/133430Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-11T01:37:50Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
title Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
spellingShingle Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
Tiago, Francisco José Martins
Cannabis sativa
Green Extraction
Deep Eutectic Solvents
Cannabinoids
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
title_short Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
title_full Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
title_fullStr Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
title_full_unstemmed Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
title_sort Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents
author Tiago, Francisco José Martins
author_facet Tiago, Francisco José Martins
author_role author
dc.contributor.none.fl_str_mv Duarte, Ana
Matias, Ana
RUN
dc.contributor.author.fl_str_mv Tiago, Francisco José Martins
dc.subject.por.fl_str_mv Cannabis sativa
Green Extraction
Deep Eutectic Solvents
Cannabinoids
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
topic Cannabis sativa
Green Extraction
Deep Eutectic Solvents
Cannabinoids
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
description The increasing demand for medical cannabis urges the development for new and effective methods for the extraction of phytocannabinoids. Deep eutectic Solvents (DESs) are an alternative to the use of hazardous organic solvents typically used in the industry. In this study, DESs were developed, based on terpenes, sugars, and natural organic acids, as a green extraction media for the extraction of cannabis bioactive compounds. The factors influencing the extraction of bioactive components, such as the type of DESs and extraction time were investigated. Initial screening in hemp showed that the DES composed of Menthol: Lauric acid (2:1 M ratio) had a greater extraction efficiency of CBD and CBDA (11,07 ± 0,37 mg/g) of all the tested DESs and higher than ethanol. In medical cannabis Men:Lac (2:1 M ratio) allowed an extraction as effective as ethanol. Besides having a higher or equivalent extraction yield as the organic solvents tested, DESs showed to be more selective extracting less impurities, such as chlorophyll and waxes. These results coupled with the non-toxic, biodegradable, low cost and environmentally friendly characteristics of DESs, provided strong evidence that DESs represents a better alternative to organic solvents.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-17
2022-02-17T00:00:00Z
2024-11-01T01:32:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133430
url http://hdl.handle.net/10362/133430
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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