Clinical Outcomes and Genetic Expression Profile in Human Liver Graft Dysfunction During Ischemia/Reperfusion Injury

• Autor(a) principal: Paulino, J
• Data de Publicação: 2015
• Outros Autores: Vigia, E, Marcelino, P, Abade, O, Sobral, J, Ligeiro, D, Carvalho, A, Alves, M, Papoila, AL, Trindade, H, Barroso, E
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.17/2292
• topic: Cold Ischemia/methods; HCC ANPAT; HCC CHBPT; Gene Expression Profiling/methods; Gene Expression Regulation; Genetic Markers/genetics; Liver Diseases/genetics; Liver Diseases/metabolism; Liver Diseases/surgery; Liver Transplantation; Microarray Analysis; RNA/genetics; Reperfusion Injury/genetics; Reperfusion Injury/metabolism; Retrospective Studies; Transplants/metabolism; Transplants/pathology

Introduction. This study aims to compare the molecular gene expression during ischemia reperfusion injury. Several surgical times were considered: in the beginning of the harvesting (T0), at the end of the cold ischemia period (T1), and after reperfusion (T2) and compared with graft dysfunction after liver transplant (OLT). Methods. We studied 54 patients undergoing OLT. Clinical, laboratory data, and histologic data (Suzuki classification) as well as the Survival Outcomes Following Liver Transplantation (SOFT) score were used and compared with the molecular gene expression of the following genes: Interleukin (IL)-1b, IL-6, tumor necrosis factor-a, perforin, E-selectin (SELE), Fas-ligand, granzyme B, heme oxygenase-1, and nitric oxide synthetase. Results. Fifteen patients presented with graft dysfunction according to SOFT criteria. No relevant data were obtained by comparing the variables graft dysfunction and histologic variables. We observed a statistically significant relation between SELE at T0 (P ¼ .013) and IL-1b at T0 (P ¼ .028) and early graft dysfunction. Conclusions. We conclude that several genetically determined proinflammatory expressions may play a critical role in the development of graft dysfunction after OLT.