Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

• Autor(a) principal: Benavente, Rocio
• Data de Publicação: 2015
• Outros Autores: Pessela, Benevides C., Curiel, Jose Antonio, de las Rivas, Blanca, Muñoz, Rosario, Guisán, Jose Manuel, Mancheño, Jose M., Cardelle-Cobas, Alejandra, Ruiz-Matute, Ana I., Corzo, Nieves
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.14/19934
• topic: β-galactosidase; Lactobacillus plantarum; Immobilization; Glyoxyl-agarose; Oligosaccharides synthesis; Lactose; Lactulose

A novel -galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-nitrophenyl -d-galactopyranoside. This value was conserved in the presence of different divalent cations and was quite resistant to the inhibition effects of different carbohydrates. The pure multimeric enzyme was stabilized by multipoint and multisubunit covalent attachment on glyoxyl-agarose. The glyoxyl-LPG immobilized preparation was over 20-fold more stable than the soluble enzyme or the one-point CNBr-LPG immobilized preparation at 50 degrees C. This -galactosidase was successfully used in the hydrolysis of lactose and lactulose and formation of different oligosaccharides was detected. High production of galacto-oligosaccharides (35%) and oligosaccharides derived from lactulose (30%) was found and, for the first time, a new oligosaccharide derived from lactulose, tentatively identified as 3'-galactosyl lactulose, has been described.