Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/47539 |
Resumo: | Cystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial composition) of dual-/three-species biofilms involving CF-classical (Pseudomonas aeruginosa) and unusual species (Inquilinus limosus and Dolosigranulum pigrum), according to variable oxygen conditions and antibiotic exposure. Low fluctuations in biofilm compositions were observed across distinct oxygen environments, with dual-species biofilms exhibiting similar relative proportions and P. aeruginosa and/or D. pigrum populations dominating three-species consortia. Once exposed to antibiotics, biofilms displayed high resistance profiles, and microbial compositions, distributions, and microbial interactions significantly challenged. The antibiotic/oxygen environment supported such fluctuations, which enhanced for three-species communities. In conclusion, antibiotic therapy hugely disturbed CF communities dynamics, inducing significant compositional changes on multispecies consortia. Clearly, multiple perturbations may disturb this dynamic, giving rise to various microbiological scenarios in vivo, and affecting disease phenotype. Therefore, an appreciation of the ecological/evolutionary nature within CF communities will be useful for the optimal use of current therapies and for newer breakthroughs on CF antibiotherapy. |
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Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stressantibiotic therapycystic fibrosisinterspecies interactionspolymicrobial biofilmsPseudomonas aeruginosaScience & TechnologyCystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial composition) of dual-/three-species biofilms involving CF-classical (Pseudomonas aeruginosa) and unusual species (Inquilinus limosus and Dolosigranulum pigrum), according to variable oxygen conditions and antibiotic exposure. Low fluctuations in biofilm compositions were observed across distinct oxygen environments, with dual-species biofilms exhibiting similar relative proportions and P. aeruginosa and/or D. pigrum populations dominating three-species consortia. Once exposed to antibiotics, biofilms displayed high resistance profiles, and microbial compositions, distributions, and microbial interactions significantly challenged. The antibiotic/oxygen environment supported such fluctuations, which enhanced for three-species communities. In conclusion, antibiotic therapy hugely disturbed CF communities dynamics, inducing significant compositional changes on multispecies consortia. Clearly, multiple perturbations may disturb this dynamic, giving rise to various microbiological scenarios in vivo, and affecting disease phenotype. Therefore, an appreciation of the ecological/evolutionary nature within CF communities will be useful for the optimal use of current therapies and for newer breakthroughs on CF antibiotherapy.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684). The authors also acknowledge the financial support provided by FCT through the projects: PTDC/SAU-ESA/646091/2006/ FCOMP01-0124-FEDER-007480FCT; strategic project PEst-OE/EQB/LA0023/2013; “BioHealth – Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE-07-0124-FEDER-000027, cofunded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER; RECI/BBB-EBI/0179/2012 – Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB, FCOMP-01-0124-FEDER-027462, FEDER; and the DNA mimics project PIC/IC/82815/2007. The FCT BPD fellowship of Susana P. Lopes SFRH/BPD/95616/2013 and the support of the COST-Action TD1004:Theragnostics for imaging and therapy is also acknowledged.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoLopes, Susana P.Azevedo, Nuno FilipePereira, Maria Olívia2017-122017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/47539engLopes, Susana P.; Azevedo, Nuno Filipe; Pereira, Maria Olívia, Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress. International Journal of Medical Microbiology, 307(8), 460-470, 20171618-06071438-422110.1016/j.ijmm.2017.09.01829033313http://www.journals.elsevier.com/international-journal-of-medical-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:23Zoai:repositorium.sdum.uminho.pt:1822/47539Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:54:41.107543Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
title |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
spellingShingle |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress Lopes, Susana P. antibiotic therapy cystic fibrosis interspecies interactions polymicrobial biofilms Pseudomonas aeruginosa Science & Technology |
title_short |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
title_full |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
title_fullStr |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
title_full_unstemmed |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
title_sort |
Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress |
author |
Lopes, Susana P. |
author_facet |
Lopes, Susana P. Azevedo, Nuno Filipe Pereira, Maria Olívia |
author_role |
author |
author2 |
Azevedo, Nuno Filipe Pereira, Maria Olívia |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Lopes, Susana P. Azevedo, Nuno Filipe Pereira, Maria Olívia |
dc.subject.por.fl_str_mv |
antibiotic therapy cystic fibrosis interspecies interactions polymicrobial biofilms Pseudomonas aeruginosa Science & Technology |
topic |
antibiotic therapy cystic fibrosis interspecies interactions polymicrobial biofilms Pseudomonas aeruginosa Science & Technology |
description |
Cystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial composition) of dual-/three-species biofilms involving CF-classical (Pseudomonas aeruginosa) and unusual species (Inquilinus limosus and Dolosigranulum pigrum), according to variable oxygen conditions and antibiotic exposure. Low fluctuations in biofilm compositions were observed across distinct oxygen environments, with dual-species biofilms exhibiting similar relative proportions and P. aeruginosa and/or D. pigrum populations dominating three-species consortia. Once exposed to antibiotics, biofilms displayed high resistance profiles, and microbial compositions, distributions, and microbial interactions significantly challenged. The antibiotic/oxygen environment supported such fluctuations, which enhanced for three-species communities. In conclusion, antibiotic therapy hugely disturbed CF communities dynamics, inducing significant compositional changes on multispecies consortia. Clearly, multiple perturbations may disturb this dynamic, giving rise to various microbiological scenarios in vivo, and affecting disease phenotype. Therefore, an appreciation of the ecological/evolutionary nature within CF communities will be useful for the optimal use of current therapies and for newer breakthroughs on CF antibiotherapy. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12 2017-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/47539 |
url |
http://hdl.handle.net/1822/47539 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lopes, Susana P.; Azevedo, Nuno Filipe; Pereira, Maria Olívia, Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress. International Journal of Medical Microbiology, 307(8), 460-470, 2017 1618-0607 1438-4221 10.1016/j.ijmm.2017.09.018 29033313 http://www.journals.elsevier.com/international-journal-of-medical-microbiology/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132329624469504 |