Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

Detalhes bibliográficos
Autor(a) principal: Almeida, Carina
Data de Publicação: 2013
Outros Autores: Azevedo, N. F., Bento, Joana C., Cerca, Nuno, Ramos, H., Vieira, M. J., Keevil, C. W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/24934
Resumo: We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
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spelling Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISHScience & TechnologyWe developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.We would like to thank Cristina Silva for providing us the urine samples. This work was supported by Fundacao para a Ciencia e Tecnologia (Postdoctoral Fellowship SFRH/BPD/74480/2010 and project PIC/IC/82815/2007) and by the European Commission's Framework Programme (FP7: "COST-Action TD1004: Theragnostics for imaging and therapy").SpringerSpringer VerlagUniversidade do MinhoAlmeida, CarinaAzevedo, N. F.Bento, Joana C.Cerca, NunoRamos, H.Vieira, M. J.Keevil, C. W.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24934eng0934-97231435-437310.1007/s10096-012-1808-223288291info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:47Zoai:repositorium.sdum.uminho.pt:1822/24934Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:03.088051Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
title Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
spellingShingle Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
Almeida, Carina
Science & Technology
title_short Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
title_full Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
title_fullStr Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
title_full_unstemmed Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
title_sort Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
author Almeida, Carina
author_facet Almeida, Carina
Azevedo, N. F.
Bento, Joana C.
Cerca, Nuno
Ramos, H.
Vieira, M. J.
Keevil, C. W.
author_role author
author2 Azevedo, N. F.
Bento, Joana C.
Cerca, Nuno
Ramos, H.
Vieira, M. J.
Keevil, C. W.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Almeida, Carina
Azevedo, N. F.
Bento, Joana C.
Cerca, Nuno
Ramos, H.
Vieira, M. J.
Keevil, C. W.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/24934
url http://hdl.handle.net/1822/24934
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0934-9723
1435-4373
10.1007/s10096-012-1808-2
23288291
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
Springer Verlag
publisher.none.fl_str_mv Springer
Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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