Protein-based nanoformulations for α-Tocopherol encapsulation

Detalhes bibliográficos
Autor(a) principal: Ghaheh, Fatemeh Shahmoradi
Data de Publicação: 2017
Outros Autores: Khoddami, Akbar, Alihosseini, Farzaneh, Gomes, Andreia, Ribeiro, Artur, Cavaco-Paulo, Artur, Silva, Carla Manuela Pereira Marinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/46389
Resumo: Nanoparticles of bovine serum albumin (BSA) and silk fibroin (SF) with entrapped α-tocopherol were produced via ultrasonic emulsification. Populations with particle size of 200300 nm and highly negatively charged were obtained for all the tested formulations. Entrapment efficiencies of around 99% revealed the effective encapsulation of α-tocopherol into the produced nanoformulations. Generally, these nanodevices did not induce significant cytotoxicity to human skin keratinocytes for all the concentrations tested. The developed formulations showed free radical scavenging of ABTS.+ ability resulting from the synergistic effect between proteins in formulation and the entrapped tocopherol. Overall, the results contribute for the establishment of BSA:VO and BSA:SF:VO as biodegradable and non-toxic nanoformulations for the functionalization of textile devices and controlled delivery of tocopherol into the skin.
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spelling Protein-based nanoformulations for α-Tocopherol encapsulationα-tocopherolanti-oxidant activityprotein-based nanoparticlesskin treatmentultrasoundsScience & TechnologyNanoparticles of bovine serum albumin (BSA) and silk fibroin (SF) with entrapped α-tocopherol were produced via ultrasonic emulsification. Populations with particle size of 200300 nm and highly negatively charged were obtained for all the tested formulations. Entrapment efficiencies of around 99% revealed the effective encapsulation of α-tocopherol into the produced nanoformulations. Generally, these nanodevices did not induce significant cytotoxicity to human skin keratinocytes for all the concentrations tested. The developed formulations showed free radical scavenging of ABTS.+ ability resulting from the synergistic effect between proteins in formulation and the entrapped tocopherol. Overall, the results contribute for the establishment of BSA:VO and BSA:SF:VO as biodegradable and non-toxic nanoformulations for the functionalization of textile devices and controlled delivery of tocopherol into the skin.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE2020 (POCI-01-0145FEDER-006684) and BioTecNorte operation (NORTE-01-0145FEDER-000004) funded by European Regional Development Fund under the scope of Norte 2020-Programa Operacional Regional do Norte. Artur Ribeiro thanks FCT for the SFRH\BPD\98388\2013 grant. Fatemeh Shahmoradi would like to acknowledge the Iran Ministry of Science, Research and Technology (MSRT) for the monetary support.info:eu-repo/semantics/publishedVersionWiley-BlackwellUniversidade do MinhoGhaheh, Fatemeh ShahmoradiKhoddami, AkbarAlihosseini, FarzanehGomes, AndreiaRibeiro, ArturCavaco-Paulo, ArturSilva, Carla Manuela Pereira Marinho2017-052017-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46389engGhaheh, Fatemeh Shahmoradi; Khoddami, Akbar; Alihosseini, Farzaneh; Gomes, Andreia; Ribeiro, Artur; Cavaco-Paulo, Artur; Silva, Carla, Protein-based nanoformulations for α-Tocopherol encapsulation. Engineering in Life Sciences, 17(5), 523-527, 20171618-02401618-286310.1002/elsc.201600188http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1618-2863info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:39Zoai:repositorium.sdum.uminho.pt:1822/46389Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:48.783566Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protein-based nanoformulations for α-Tocopherol encapsulation
title Protein-based nanoformulations for α-Tocopherol encapsulation
spellingShingle Protein-based nanoformulations for α-Tocopherol encapsulation
Ghaheh, Fatemeh Shahmoradi
α-tocopherol
anti-oxidant activity
protein-based nanoparticles
skin treatment
ultrasounds
Science & Technology
title_short Protein-based nanoformulations for α-Tocopherol encapsulation
title_full Protein-based nanoformulations for α-Tocopherol encapsulation
title_fullStr Protein-based nanoformulations for α-Tocopherol encapsulation
title_full_unstemmed Protein-based nanoformulations for α-Tocopherol encapsulation
title_sort Protein-based nanoformulations for α-Tocopherol encapsulation
author Ghaheh, Fatemeh Shahmoradi
author_facet Ghaheh, Fatemeh Shahmoradi
Khoddami, Akbar
Alihosseini, Farzaneh
Gomes, Andreia
Ribeiro, Artur
Cavaco-Paulo, Artur
Silva, Carla Manuela Pereira Marinho
author_role author
author2 Khoddami, Akbar
Alihosseini, Farzaneh
Gomes, Andreia
Ribeiro, Artur
Cavaco-Paulo, Artur
Silva, Carla Manuela Pereira Marinho
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ghaheh, Fatemeh Shahmoradi
Khoddami, Akbar
Alihosseini, Farzaneh
Gomes, Andreia
Ribeiro, Artur
Cavaco-Paulo, Artur
Silva, Carla Manuela Pereira Marinho
dc.subject.por.fl_str_mv α-tocopherol
anti-oxidant activity
protein-based nanoparticles
skin treatment
ultrasounds
Science & Technology
topic α-tocopherol
anti-oxidant activity
protein-based nanoparticles
skin treatment
ultrasounds
Science & Technology
description Nanoparticles of bovine serum albumin (BSA) and silk fibroin (SF) with entrapped α-tocopherol were produced via ultrasonic emulsification. Populations with particle size of 200300 nm and highly negatively charged were obtained for all the tested formulations. Entrapment efficiencies of around 99% revealed the effective encapsulation of α-tocopherol into the produced nanoformulations. Generally, these nanodevices did not induce significant cytotoxicity to human skin keratinocytes for all the concentrations tested. The developed formulations showed free radical scavenging of ABTS.+ ability resulting from the synergistic effect between proteins in formulation and the entrapped tocopherol. Overall, the results contribute for the establishment of BSA:VO and BSA:SF:VO as biodegradable and non-toxic nanoformulations for the functionalization of textile devices and controlled delivery of tocopherol into the skin.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
2017-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/46389
url http://hdl.handle.net/1822/46389
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ghaheh, Fatemeh Shahmoradi; Khoddami, Akbar; Alihosseini, Farzaneh; Gomes, Andreia; Ribeiro, Artur; Cavaco-Paulo, Artur; Silva, Carla, Protein-based nanoformulations for α-Tocopherol encapsulation. Engineering in Life Sciences, 17(5), 523-527, 2017
1618-0240
1618-2863
10.1002/elsc.201600188
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1618-2863
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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