Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/1387 |
Resumo: | By applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose. |
id |
RCAP_30658c27ebb1e33d46ce508e91abf6d8 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/1387 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharidesHazelnutGevuina avellanaSeed structureCell wallsLight microscopyFT-IRXyloglucanScience & TechnologyBy applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose.Fundação para a Ciência e Tecnologia (FCT) - PRAXIS XXI/BPD/18824/99. Research Unit 62/94, QOPNA (Aveiro, Portugal). INCO-DC 96-2205 (OLNOCO).Society of Chemical IndustryUniversidade do MinhoDourado, FernandoVasco, PedroBarros, AntónioMota, M.Coimbra, Manuel A.Gama, F. M.20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/1387engDourado, F., Vasco, P., Barros, A., Mota, M., Coimbra, M.A. and Gama, F.M. (2003), Characterisation of Chilean hazelnut (Gevuina avellana) tissues: light microscopy and cell wall polysaccharides. J. Sci. Food Agric., 83: 158-165. https://doi.org/10.1002/jsfa.12870022-514210.1002/jsfa.1287https://onlinelibrary.wiley.com/doi/10.1002/jsfa.1287info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:27Zoai:repositorium.sdum.uminho.pt:1822/1387Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:34:53.307206Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
title |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
spellingShingle |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides Dourado, Fernando Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan Science & Technology |
title_short |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
title_full |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
title_fullStr |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
title_full_unstemmed |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
title_sort |
Characterisation of chilean hazelnut (gevuina avellana) tissues : light microscopy and cell wall polysaccharides |
author |
Dourado, Fernando |
author_facet |
Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
author_role |
author |
author2 |
Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Dourado, Fernando Vasco, Pedro Barros, António Mota, M. Coimbra, Manuel A. Gama, F. M. |
dc.subject.por.fl_str_mv |
Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan Science & Technology |
topic |
Hazelnut Gevuina avellana Seed structure Cell walls Light microscopy FT-IR Xyloglucan Science & Technology |
description |
By applying several differential staining techniques and light microscopy, the structure and composition of Chilean hazelnut (Gevuina avellana) seeds were analysed. The structure of the G avellana seed is very simple, with a thin, heavily lignified seed coat and two voluminous cotyledons. The embryo food reserves are uniformly distributed over the cotyledon cells. The cell wall polysaccharides were recovered from the alcohol-insoluble residue by mild treatment with warm chlorite solution and sequential extraction with alkali solutions of increasing concentration. FT-IR spectra in the 1200–850 cm-1 region were used together with chemometric techniques to distinguish the hemicellulosic and pectic polysaccharides in the extracts. The most abundant extracts were fractionated by graded precipitation in ethanol. A xyloglucan was identified by 1H and 13C NMR as the major hemicellulosic polysaccharide, with a sugar composition of 4Glc:3.5Xyl:1Gal:0.5Fuc. The hazelnut cell walls are composed of equivalent amounts of pectic polysaccharides, xyloglucans and cellulose. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2003-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/1387 |
url |
https://hdl.handle.net/1822/1387 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dourado, F., Vasco, P., Barros, A., Mota, M., Coimbra, M.A. and Gama, F.M. (2003), Characterisation of Chilean hazelnut (Gevuina avellana) tissues: light microscopy and cell wall polysaccharides. J. Sci. Food Agric., 83: 158-165. https://doi.org/10.1002/jsfa.1287 0022-5142 10.1002/jsfa.1287 https://onlinelibrary.wiley.com/doi/10.1002/jsfa.1287 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society of Chemical Industry |
publisher.none.fl_str_mv |
Society of Chemical Industry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132872384184320 |