Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/74010 |
Resumo: | Biosorbent materials are effective in the removal of spilled oil from water, but their effect on hydrocarbonoclastic bacteria is not known. Here, we show that corksorb, a cork-based biosorbent, enhances growth and alkane degradation by Rhodococcus opacus B4 (Ro) and Alcanivorax borkumensis SK2 (Ab). Ro and Ab degraded 96 ± 1% and 72 ± 2%, respectively, of a mixture of n-alkanes (2 g L1) in the presence of corksorb. These values represent an increase of 6 and 24%, respectively, relative to the assays without corksorb. The biosorbent also increased the growth of Ab by 51%. However, no significant changes were detected in the expression of genes involved in alkane uptake and degradation in the presence of corksorb relative to the control without the biosorbent. Nevertheless, transcriptomics analysis revealed an increased expression of rRNA and tRNA coding genes, which confirms the higher metabolic activity of Ab in the presence of corksorb. The effect of corksorb is not related to the release of soluble stimulating compounds, but rather to the presence of the biosorbent, which was shown to be essential. Indeed, scanning electron microscopy images and downregulation of pili formation coding genes, which are involved in cell mobility, suggest that cell attachment on corksorb is a determinant for the improved activity. Furthermore, the existence of native alkane-degrading bacteria in corksorb was revealed, which may assist in situ bioremediation. Hence, the use of corksorb in marine oil spills may induce a combined effect of sorption and stimulated biodegradation, with high potential for enhancing in situ bioremediation processes. © Copyright © 2021 Martins, Freitas, Castro, Silva, Gudiña, Sequeira, Salvador, Pereira and Cavaleiro. |
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Corksorb enhances alkane degradation by hydrocarbonoclastic bacteriaAlcanivorax borkumensisAlkanesBioremediationBiosorbentComparative transcriptomicsCorksorbGrowthRhodococcus opacusScience & TechnologyBiosorbent materials are effective in the removal of spilled oil from water, but their effect on hydrocarbonoclastic bacteria is not known. Here, we show that corksorb, a cork-based biosorbent, enhances growth and alkane degradation by Rhodococcus opacus B4 (Ro) and Alcanivorax borkumensis SK2 (Ab). Ro and Ab degraded 96 ± 1% and 72 ± 2%, respectively, of a mixture of n-alkanes (2 g L1) in the presence of corksorb. These values represent an increase of 6 and 24%, respectively, relative to the assays without corksorb. The biosorbent also increased the growth of Ab by 51%. However, no significant changes were detected in the expression of genes involved in alkane uptake and degradation in the presence of corksorb relative to the control without the biosorbent. Nevertheless, transcriptomics analysis revealed an increased expression of rRNA and tRNA coding genes, which confirms the higher metabolic activity of Ab in the presence of corksorb. The effect of corksorb is not related to the release of soluble stimulating compounds, but rather to the presence of the biosorbent, which was shown to be essential. Indeed, scanning electron microscopy images and downregulation of pili formation coding genes, which are involved in cell mobility, suggest that cell attachment on corksorb is a determinant for the improved activity. Furthermore, the existence of native alkane-degrading bacteria in corksorb was revealed, which may assist in situ bioremediation. Hence, the use of corksorb in marine oil spills may induce a combined effect of sorption and stimulated biodegradation, with high potential for enhancing in situ bioremediation processes. © Copyright © 2021 Martins, Freitas, Castro, Silva, Gudiña, Sequeira, Salvador, Pereira and Cavaleiro.This study was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of project MORE (POCI-01-0145-FEDER-016575) and Salt Oil+ (POCI-01-0145-FEDER-030180) and of the strategic funding of UIDB/04469/2020 unit. Research of RS and JS was supported by Ph.D. grants SFRH/BD/116154/2016 and SFRH/BD/147271/2019, respectively, funded by FCT.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoMartins, Valdo R.Freitas, C.Castro, RitaSilva, Ana Rita MarquesGudiña, Eduardo JoséSequeira, J. C.Salvador, Andreia Filipa FerreiraPereira, M. A.Cavaleiro, Ana Júlia2021-08-192021-08-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/74010engMartins, Valdo R.; Freitas, C.; Castro, Rita; Silva, Ana Rita; Gudiña, Eduardo J.; Sequeira, J. C.; Salvador, Andreia F.; Pereira, M. Alcina; Cavaleiro, Ana Júlia, Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria. Frontiers in Microbiology, 12(618270), 20211664-302X10.3389/fmicb.2021.618270618270https://www.frontiersin.org/articles/10.3389/fmicb.2021.618270/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:35Zoai:repositorium.sdum.uminho.pt:1822/74010Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:54:53.717396Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
title |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
spellingShingle |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria Martins, Valdo R. Alcanivorax borkumensis Alkanes Bioremediation Biosorbent Comparative transcriptomics Corksorb Growth Rhodococcus opacus Science & Technology |
title_short |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
title_full |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
title_fullStr |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
title_full_unstemmed |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
title_sort |
Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria |
author |
Martins, Valdo R. |
author_facet |
Martins, Valdo R. Freitas, C. Castro, Rita Silva, Ana Rita Marques Gudiña, Eduardo José Sequeira, J. C. Salvador, Andreia Filipa Ferreira Pereira, M. A. Cavaleiro, Ana Júlia |
author_role |
author |
author2 |
Freitas, C. Castro, Rita Silva, Ana Rita Marques Gudiña, Eduardo José Sequeira, J. C. Salvador, Andreia Filipa Ferreira Pereira, M. A. Cavaleiro, Ana Júlia |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Valdo R. Freitas, C. Castro, Rita Silva, Ana Rita Marques Gudiña, Eduardo José Sequeira, J. C. Salvador, Andreia Filipa Ferreira Pereira, M. A. Cavaleiro, Ana Júlia |
dc.subject.por.fl_str_mv |
Alcanivorax borkumensis Alkanes Bioremediation Biosorbent Comparative transcriptomics Corksorb Growth Rhodococcus opacus Science & Technology |
topic |
Alcanivorax borkumensis Alkanes Bioremediation Biosorbent Comparative transcriptomics Corksorb Growth Rhodococcus opacus Science & Technology |
description |
Biosorbent materials are effective in the removal of spilled oil from water, but their effect on hydrocarbonoclastic bacteria is not known. Here, we show that corksorb, a cork-based biosorbent, enhances growth and alkane degradation by Rhodococcus opacus B4 (Ro) and Alcanivorax borkumensis SK2 (Ab). Ro and Ab degraded 96 ± 1% and 72 ± 2%, respectively, of a mixture of n-alkanes (2 g L1) in the presence of corksorb. These values represent an increase of 6 and 24%, respectively, relative to the assays without corksorb. The biosorbent also increased the growth of Ab by 51%. However, no significant changes were detected in the expression of genes involved in alkane uptake and degradation in the presence of corksorb relative to the control without the biosorbent. Nevertheless, transcriptomics analysis revealed an increased expression of rRNA and tRNA coding genes, which confirms the higher metabolic activity of Ab in the presence of corksorb. The effect of corksorb is not related to the release of soluble stimulating compounds, but rather to the presence of the biosorbent, which was shown to be essential. Indeed, scanning electron microscopy images and downregulation of pili formation coding genes, which are involved in cell mobility, suggest that cell attachment on corksorb is a determinant for the improved activity. Furthermore, the existence of native alkane-degrading bacteria in corksorb was revealed, which may assist in situ bioremediation. Hence, the use of corksorb in marine oil spills may induce a combined effect of sorption and stimulated biodegradation, with high potential for enhancing in situ bioremediation processes. © Copyright © 2021 Martins, Freitas, Castro, Silva, Gudiña, Sequeira, Salvador, Pereira and Cavaleiro. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-19 2021-08-19T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/74010 |
url |
https://hdl.handle.net/1822/74010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, Valdo R.; Freitas, C.; Castro, Rita; Silva, Ana Rita; Gudiña, Eduardo J.; Sequeira, J. C.; Salvador, Andreia F.; Pereira, M. Alcina; Cavaleiro, Ana Júlia, Corksorb enhances alkane degradation by hydrocarbonoclastic bacteria. Frontiers in Microbiology, 12(618270), 2021 1664-302X 10.3389/fmicb.2021.618270 618270 https://www.frontiersin.org/articles/10.3389/fmicb.2021.618270/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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