Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections

Detalhes bibliográficos
Autor(a) principal: Friães, Ana
Data de Publicação: 2015
Outros Autores: Pato, Catarina, Cristino, José Melo, Ramirez, Mário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49168
Resumo: Copyright © 2015, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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spelling Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infectionsCopyright © 2015, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/To evaluate the importance of covRS and ropB mutations in invasive disease caused by Group A Streptococci (GAS), we determined the sequence of the covRS and ropB genes of 191 isolates from invasive infections and pharyngitis, comprising a diverse set of emm types and multilocus sequence types. The production of SpeB and the activity of NAD glycohydrolase (NADase) and streptolysin S (SLS) were evaluated. The results support the acquisition of null covS alleles (predicted to eliminate protein function), resulting in downregulation of SpeB and upregulation of NADase and SLS, as a mechanism possibly contributing to higher invasiveness. Among the isolates tested, this mechanism was found to be uncommon (10% of invasive isolates) and was not more prevalent among clones with enhanced invasiveness (including M1T1) but occurred in diverse genetic backgrounds. In lineages such as emm64, these changes did not result in upregulation of NADase and SLS, highlighting the diversity of regulatory pathways in GAS. Despite abrogating SpeB production, null alleles in ropB were not associated with invasive infection. The covRS and ropB genes are under stabilising selection and no expansion of isolates carrying null alleles has been observed, suggesting that the presence of these regulators is important for overall fitness.Springer NatureRepositório da Universidade de LisboaFriães, AnaPato, CatarinaCristino, José MeloRamirez, Mário2021-07-27T13:57:51Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49168engSci Rep. 2015 Jul 15;5:1205710.1038/srep120572045-2322info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:35Zoai:repositorio.ul.pt:10451/49168Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:43.880952Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
title Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
spellingShingle Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
Friães, Ana
title_short Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
title_full Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
title_fullStr Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
title_full_unstemmed Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
title_sort Consequences of the variability of the CovRS and RopB regulators among Streptococcus pyogenes causing human infections
author Friães, Ana
author_facet Friães, Ana
Pato, Catarina
Cristino, José Melo
Ramirez, Mário
author_role author
author2 Pato, Catarina
Cristino, José Melo
Ramirez, Mário
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Friães, Ana
Pato, Catarina
Cristino, José Melo
Ramirez, Mário
description Copyright © 2015, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2021-07-27T13:57:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49168
url http://hdl.handle.net/10451/49168
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sci Rep. 2015 Jul 15;5:12057
10.1038/srep12057
2045-2322
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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