Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/42993 |
Resumo: | Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1â 3 μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45 μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membraneâ s mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration. |
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Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineeringBM-MSCsMineralisationOsteogenic differentiationPLLA membranesStrontium borosilicate glassesScience & TechnologyHerein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1â 3 μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45 μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membraneâ s mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration. The authors gratefully acknowledge financial support from Portuguese Foundation for Science and Technology (Ph.D. grant BD/73162/2010), the European Union’s Seventh Framework Programme (FP7/2007–2013) under Grant No. REGPOT-CT2012- 31633-POLARIS. This work was also supported by the European Research Council grant agreement ERC-2012-ADG-20120216- 321266 for the project ComplexiTE and UK EPSRC Centre for Innovative Manufacturing of Medical Devices-MeDe Innovation (EPSRC grant EP/K029592/1).ElsevierUniversidade do MinhoFernandes, João S.Gentile, PiergiorgioMartins, Margarida Isabel Barros CoelhoNeves, N. M.Miller, CherylCrawford, AileenPires, R. A.Hatton, PaulReis, R. L.2016-102016-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42993engFernandes J. S., Gentile P., Martins I. M., Neves N. M., Miller C., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Reinforcement of Poly-L-lactic acid Electrospun Membranes with Strontium Borosilicate Bioactive Glasses for Bone Tissue Engineering, Acta Biomaterialia, Vol. 44, pp. 168-177, doi:10.1016/j.actbio.2016.08.042, 20161742-706110.1016/j.actbio.2016.08.04227554018http://www.sciencedirect.com/science/article/pii/S174270611630438Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:02Zoai:repositorium.sdum.uminho.pt:1822/42993Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:25.210868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
title |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
spellingShingle |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering Fernandes, João S. BM-MSCs Mineralisation Osteogenic differentiation PLLA membranes Strontium borosilicate glasses Science & Technology |
title_short |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
title_full |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
title_fullStr |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
title_full_unstemmed |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
title_sort |
Reinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering |
author |
Fernandes, João S. |
author_facet |
Fernandes, João S. Gentile, Piergiorgio Martins, Margarida Isabel Barros Coelho Neves, N. M. Miller, Cheryl Crawford, Aileen Pires, R. A. Hatton, Paul Reis, R. L. |
author_role |
author |
author2 |
Gentile, Piergiorgio Martins, Margarida Isabel Barros Coelho Neves, N. M. Miller, Cheryl Crawford, Aileen Pires, R. A. Hatton, Paul Reis, R. L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fernandes, João S. Gentile, Piergiorgio Martins, Margarida Isabel Barros Coelho Neves, N. M. Miller, Cheryl Crawford, Aileen Pires, R. A. Hatton, Paul Reis, R. L. |
dc.subject.por.fl_str_mv |
BM-MSCs Mineralisation Osteogenic differentiation PLLA membranes Strontium borosilicate glasses Science & Technology |
topic |
BM-MSCs Mineralisation Osteogenic differentiation PLLA membranes Strontium borosilicate glasses Science & Technology |
description |
Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1â 3 μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45 μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membraneâ s mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 2016-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42993 |
url |
http://hdl.handle.net/1822/42993 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes J. S., Gentile P., Martins I. M., Neves N. M., Miller C., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Reinforcement of Poly-L-lactic acid Electrospun Membranes with Strontium Borosilicate Bioactive Glasses for Bone Tissue Engineering, Acta Biomaterialia, Vol. 44, pp. 168-177, doi:10.1016/j.actbio.2016.08.042, 2016 1742-7061 10.1016/j.actbio.2016.08.042 27554018 http://www.sciencedirect.com/science/article/pii/S174270611630438X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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