Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/4066 |
Resumo: | ABSTRACT: Microalga Chlorella protothecoides materials were assessed as substrates for anaerobic digestion (AD) aiming at the simultaneous production of biogas/methane and pigments: whole autotrophic (AA) and heterotrophic algae (H); extracted heterotrophic microalgae from lipid production (HExt); and pretreated heterotrophic microalgae through enzymatic (HPEnz), autoclave (HPA), and ultrasound (HPU) processes. AA was more suitable for AD than H, as it was more efficiently converted into methane (279 vs. 180 L CH4/kg VSin). In comparison, the pretreatment of heterotrophic microalgae had a positive effect on AD, with registered methane yield increases from 263 to 290 L CH4/kg VSin (HPU, HPA, HExt). Reddish pigmentation developed in H and HPU units due to the presence of purple non-sulfur bacteria (PNSB). This phenomenon and the changes in microbiota structure during AD were confirmed by metagenomic analysis. At the end of the process, the relative abundance of Clostridiales and Bacillales increased, enhancing the hydrolysis of compounds in acetate. Consistently, Methanosaeta became the comparatively dominant methanogen, meaning that methane was produced through the acetoclastic methanogenesis pathway. The obtained results indicate for AD biorefinery feasibility-regarding the simultaneous production of biogas/methane-a digestate flow and pigments (bacteriochlorophyll a and carotenoids). |
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Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored DigestateMicroalgaeChlorella protothecoidesPretreatmentsAnaerobic digestionABSTRACT: Microalga Chlorella protothecoides materials were assessed as substrates for anaerobic digestion (AD) aiming at the simultaneous production of biogas/methane and pigments: whole autotrophic (AA) and heterotrophic algae (H); extracted heterotrophic microalgae from lipid production (HExt); and pretreated heterotrophic microalgae through enzymatic (HPEnz), autoclave (HPA), and ultrasound (HPU) processes. AA was more suitable for AD than H, as it was more efficiently converted into methane (279 vs. 180 L CH4/kg VSin). In comparison, the pretreatment of heterotrophic microalgae had a positive effect on AD, with registered methane yield increases from 263 to 290 L CH4/kg VSin (HPU, HPA, HExt). Reddish pigmentation developed in H and HPU units due to the presence of purple non-sulfur bacteria (PNSB). This phenomenon and the changes in microbiota structure during AD were confirmed by metagenomic analysis. At the end of the process, the relative abundance of Clostridiales and Bacillales increased, enhancing the hydrolysis of compounds in acetate. Consistently, Methanosaeta became the comparatively dominant methanogen, meaning that methane was produced through the acetoclastic methanogenesis pathway. The obtained results indicate for AD biorefinery feasibility-regarding the simultaneous production of biogas/methane-a digestate flow and pigments (bacteriochlorophyll a and carotenoids).MDPIRepositório do LNEGEusebio, AnaSantos, Carla A.Marques, Isabel Paula2023-06-28T15:24:38Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/4066engEusébio, Ana... [et.al.] - Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate. In: Applied Sciences, 2023, vol. 13 (5), article nº 332510.3390/app130533252076-3417info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-02T06:25:57Zoai:repositorio.lneg.pt:10400.9/4066Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:02:16.766197Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
title |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
spellingShingle |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate Eusebio, Ana Microalgae Chlorella protothecoides Pretreatments Anaerobic digestion |
title_short |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
title_full |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
title_fullStr |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
title_full_unstemmed |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
title_sort |
Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate |
author |
Eusebio, Ana |
author_facet |
Eusebio, Ana Santos, Carla A. Marques, Isabel Paula |
author_role |
author |
author2 |
Santos, Carla A. Marques, Isabel Paula |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Eusebio, Ana Santos, Carla A. Marques, Isabel Paula |
dc.subject.por.fl_str_mv |
Microalgae Chlorella protothecoides Pretreatments Anaerobic digestion |
topic |
Microalgae Chlorella protothecoides Pretreatments Anaerobic digestion |
description |
ABSTRACT: Microalga Chlorella protothecoides materials were assessed as substrates for anaerobic digestion (AD) aiming at the simultaneous production of biogas/methane and pigments: whole autotrophic (AA) and heterotrophic algae (H); extracted heterotrophic microalgae from lipid production (HExt); and pretreated heterotrophic microalgae through enzymatic (HPEnz), autoclave (HPA), and ultrasound (HPU) processes. AA was more suitable for AD than H, as it was more efficiently converted into methane (279 vs. 180 L CH4/kg VSin). In comparison, the pretreatment of heterotrophic microalgae had a positive effect on AD, with registered methane yield increases from 263 to 290 L CH4/kg VSin (HPU, HPA, HExt). Reddish pigmentation developed in H and HPU units due to the presence of purple non-sulfur bacteria (PNSB). This phenomenon and the changes in microbiota structure during AD were confirmed by metagenomic analysis. At the end of the process, the relative abundance of Clostridiales and Bacillales increased, enhancing the hydrolysis of compounds in acetate. Consistently, Methanosaeta became the comparatively dominant methanogen, meaning that methane was produced through the acetoclastic methanogenesis pathway. The obtained results indicate for AD biorefinery feasibility-regarding the simultaneous production of biogas/methane-a digestate flow and pigments (bacteriochlorophyll a and carotenoids). |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-28T15:24:38Z 2023-03 2023-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/4066 |
url |
http://hdl.handle.net/10400.9/4066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eusébio, Ana... [et.al.] - Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate. In: Applied Sciences, 2023, vol. 13 (5), article nº 3325 10.3390/app13053325 2076-3417 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131689298952192 |