Evaluation of the antioxidant activity of cell extracts from microalgae
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/14310 |
Resumo: | A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract. |
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Evaluation of the antioxidant activity of cell extracts from microalgae16S rDNAABTSPhage P22/SalmonellaDNAAmes testA growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaGuedes, A. CatarinaGião, Maria S.Seabra, RuiFerreira, AntonioTamagnini, PaulaMoradas-Ferreira, PedroMalcata, F. Xavier2014-05-08T12:41:24Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/14310engGUEDES, A. Catarina ...[et al.] - Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Marine Drugs. ISSN 1660-3397. Vol. 11 (2013), p. 1256-12701660-339710.3390/md11041256info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:18:57Zoai:repositorio.ucp.pt:10400.14/14310Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:11:31.346130Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the antioxidant activity of cell extracts from microalgae |
title |
Evaluation of the antioxidant activity of cell extracts from microalgae |
spellingShingle |
Evaluation of the antioxidant activity of cell extracts from microalgae Guedes, A. Catarina 16S rDNA ABTS Phage P22/Salmonella DNA Ames test |
title_short |
Evaluation of the antioxidant activity of cell extracts from microalgae |
title_full |
Evaluation of the antioxidant activity of cell extracts from microalgae |
title_fullStr |
Evaluation of the antioxidant activity of cell extracts from microalgae |
title_full_unstemmed |
Evaluation of the antioxidant activity of cell extracts from microalgae |
title_sort |
Evaluation of the antioxidant activity of cell extracts from microalgae |
author |
Guedes, A. Catarina |
author_facet |
Guedes, A. Catarina Gião, Maria S. Seabra, Rui Ferreira, Antonio Tamagnini, Paula Moradas-Ferreira, Pedro Malcata, F. Xavier |
author_role |
author |
author2 |
Gião, Maria S. Seabra, Rui Ferreira, Antonio Tamagnini, Paula Moradas-Ferreira, Pedro Malcata, F. Xavier |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Guedes, A. Catarina Gião, Maria S. Seabra, Rui Ferreira, Antonio Tamagnini, Paula Moradas-Ferreira, Pedro Malcata, F. Xavier |
dc.subject.por.fl_str_mv |
16S rDNA ABTS Phage P22/Salmonella DNA Ames test |
topic |
16S rDNA ABTS Phage P22/Salmonella DNA Ames test |
description |
A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2014-05-08T12:41:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/14310 |
url |
http://hdl.handle.net/10400.14/14310 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
GUEDES, A. Catarina ...[et al.] - Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Marine Drugs. ISSN 1660-3397. Vol. 11 (2013), p. 1256-1270 1660-3397 10.3390/md11041256 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131791504703488 |