Natural deep eutectic systems as alternative nontoxic cryoprotective agents

Detalhes bibliográficos
Autor(a) principal: Castro, Vânia I. B.
Data de Publicação: 2018
Outros Autores: Craveiro, Rita, Silva, Joana M., Reis, Rui L., Paiva, Alexandre, Duarte, Ana Rita C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1016/j.cryobiol.2018.06.010
Resumo: The research leading to these results has received funding provided by Portuguese Foundation for Science and Technology (FCT), through project PTDC/BBB-EBB/1676/2014 - DesZyme. Funding from the European Union Horizon 2020 program has also been, granted through the project Des.solve (ERC consolidator), ERC-2016-COG 725034. J.M.S. would also like to acknowledge the financial support by the FCT through the post-doctoral grant with reference number SFRH/BPD/116779/2016.
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spelling Natural deep eutectic systems as alternative nontoxic cryoprotective agentsCryoprotectantsFreeze-thawingNatural deep eutectic solventsSugarsBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)The research leading to these results has received funding provided by Portuguese Foundation for Science and Technology (FCT), through project PTDC/BBB-EBB/1676/2014 - DesZyme. Funding from the European Union Horizon 2020 program has also been, granted through the project Des.solve (ERC consolidator), ERC-2016-COG 725034. J.M.S. would also like to acknowledge the financial support by the FCT through the post-doctoral grant with reference number SFRH/BPD/116779/2016.Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with extreme temperature amplitudes, being responsible for their survival at negative temperatures during winter. Herein, we report for the first time the use of NADES based on trehalose (Treh) and glycerol (Gly) in cryopreservation, as cryoprotective agents (CPA). The evaluation of the thermal behaviour of these eutectic systems, showed that NADES have a strong effect on the water crystallization/freezing and melting process, being able to reduce the number of ice crystals and hence ice crystal damage in cells, which is a crucial parameter for their survival, upon freezing. Using this NADES as CPA, it is possible to achieve similar or even better cellular performance when compared with the gold standard for cryopreservation dimethyl sulfoxide (DMSO). In this sense, this work relates the physical properties of the NADES with their biological performance in cryopreservation. Our comprehensive strategy results in the demonstration of NADES as a promising nontoxic green alternative to the conventional CPA's used in cryopreservation methods.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNCastro, Vânia I. B.Craveiro, RitaSilva, Joana M.Reis, Rui L.Paiva, AlexandreDuarte, Ana Rita C.2019-11-05T23:43:09Z2018-08-012018-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttps://doi.org/10.1016/j.cryobiol.2018.06.010eng0011-2240PURE: 6589915http://www.scopus.com/inward/record.url?scp=85049003774&partnerID=8YFLogxKhttps://doi.org/10.1016/j.cryobiol.2018.06.010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:38:46Zoai:run.unl.pt:10362/86498Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:41.521873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Natural deep eutectic systems as alternative nontoxic cryoprotective agents
title Natural deep eutectic systems as alternative nontoxic cryoprotective agents
spellingShingle Natural deep eutectic systems as alternative nontoxic cryoprotective agents
Castro, Vânia I. B.
Cryoprotectants
Freeze-thawing
Natural deep eutectic solvents
Sugars
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
title_short Natural deep eutectic systems as alternative nontoxic cryoprotective agents
title_full Natural deep eutectic systems as alternative nontoxic cryoprotective agents
title_fullStr Natural deep eutectic systems as alternative nontoxic cryoprotective agents
title_full_unstemmed Natural deep eutectic systems as alternative nontoxic cryoprotective agents
title_sort Natural deep eutectic systems as alternative nontoxic cryoprotective agents
author Castro, Vânia I. B.
author_facet Castro, Vânia I. B.
Craveiro, Rita
Silva, Joana M.
Reis, Rui L.
Paiva, Alexandre
Duarte, Ana Rita C.
author_role author
author2 Craveiro, Rita
Silva, Joana M.
Reis, Rui L.
Paiva, Alexandre
Duarte, Ana Rita C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Castro, Vânia I. B.
Craveiro, Rita
Silva, Joana M.
Reis, Rui L.
Paiva, Alexandre
Duarte, Ana Rita C.
dc.subject.por.fl_str_mv Cryoprotectants
Freeze-thawing
Natural deep eutectic solvents
Sugars
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
topic Cryoprotectants
Freeze-thawing
Natural deep eutectic solvents
Sugars
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
description The research leading to these results has received funding provided by Portuguese Foundation for Science and Technology (FCT), through project PTDC/BBB-EBB/1676/2014 - DesZyme. Funding from the European Union Horizon 2020 program has also been, granted through the project Des.solve (ERC consolidator), ERC-2016-COG 725034. J.M.S. would also like to acknowledge the financial support by the FCT through the post-doctoral grant with reference number SFRH/BPD/116779/2016.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
2018-08-01T00:00:00Z
2019-11-05T23:43:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.cryobiol.2018.06.010
url https://doi.org/10.1016/j.cryobiol.2018.06.010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0011-2240
PURE: 6589915
http://www.scopus.com/inward/record.url?scp=85049003774&partnerID=8YFLogxK
https://doi.org/10.1016/j.cryobiol.2018.06.010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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