Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding

Detalhes bibliográficos
Autor(a) principal: Santos, David Miguel Ribeiro Vieira
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/17825
Resumo: Mestrado em Engenharia Zootécnica - Produção Animal - Instituto Superior de Agronomia / Faculdade de Medicina Veterinária
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spelling Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feedingmineralsamino acidsseasonal weight losslambsMestrado em Engenharia Zootécnica - Produção Animal - Instituto Superior de Agronomia / Faculdade de Medicina VeterináriaSeasonal weight loss (SWL) is a major constraint in extensive animal production systems of the tropics and the Mediterranean, namely for the Australian sheep production that is based on merino sheep, a European breed sensitive to SWL. SWL-tolerant alternative breeds such as the fat-tailed Damara and the Dorper have been increasingly used in Australia, due to their hardiness. Previous studies have demonstrated the impact that feed restriction has on the muscle and liver mineral and amino acid profile. The aim of this thesis is to understand the profiles of these tissues of Australian Merino, Damara and Dorper, when subjected to Seasonal Weight Loss and by comparison to control animals. Twenty-four lambs per breed were divided between growing and nutritionally restricted groups. The trial lasted for 42 days in which bi-weekly weights were registered to evaluate growth performance. At the end of the trial, animals were slaughtered. Liver and muscle samples were taken immediately after and snap frozen in liquid nitrogen. Mineral assessment as carried out using Inductively Coupled Plasma – Optical Emission Spectrometry. Amino acid profiling was achieved through High Performance Liquid Chromatography. Two-factor variance analysis was carried out using the SAS software to detect statistically significant differences resulting from the effect of breed, feeding intensity and the interaction between them. Overall, mineral concentration was lowered in the muscle of restricted animals, as a consequence of fat tissue mobilization. Increase in certain mineral concentrations such as zinc and iron might indicate increase of enzymatic activity. Outstanding concentrations of Cu in the liver of Dorper indicates greater ability to accumulate this element, even when seasonal weight loss was induced. Weight losing animals accumulate more ferritin when under weight loss. It also induced generalized muscle protein breakdown among restricted groups of all breeds with varying intensity. Dorper breed seems to mobilize less muscle amino acids when under these conditions, with the Damara having frequent significant differences. This may indicate yet another mechanism that provides the Damara with the necessary tools to endure harsh conditions. Amino acid concentration in the liver confirmed gluconeogenesis triggered by poor nutrition. Overall, the Damara demonstrated to be less susceptible to nutritional stress, the Dorper having intermediate classification and Merino the highest susceptibilityISA/ULAlmeida, André MartinhoMourato, Miguel BarbosaRepositório da Universidade de LisboaSantos, David Miguel Ribeiro Vieira2022-06-01T00:32:06Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/17825TID:203085167engSantos, D.M.R.V. - Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding. Lisboa: ISA, 2018, 107 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:28Zoai:www.repository.utl.pt:10400.5/17825Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:02:59.319920Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
title Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
spellingShingle Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
Santos, David Miguel Ribeiro Vieira
minerals
amino acids
seasonal weight loss
lambs
title_short Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
title_full Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
title_fullStr Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
title_full_unstemmed Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
title_sort Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding
author Santos, David Miguel Ribeiro Vieira
author_facet Santos, David Miguel Ribeiro Vieira
author_role author
dc.contributor.none.fl_str_mv Almeida, André Martinho
Mourato, Miguel Barbosa
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, David Miguel Ribeiro Vieira
dc.subject.por.fl_str_mv minerals
amino acids
seasonal weight loss
lambs
topic minerals
amino acids
seasonal weight loss
lambs
description Mestrado em Engenharia Zootécnica - Produção Animal - Instituto Superior de Agronomia / Faculdade de Medicina Veterinária
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2022-06-01T00:32:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/17825
TID:203085167
url http://hdl.handle.net/10400.5/17825
identifier_str_mv TID:203085167
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos, D.M.R.V. - Mineral and amino acid profiling in muscle and hepatic tissues of Damara, Dorper and Australian Merino lambs under restricted feeding. Lisboa: ISA, 2018, 107 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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