Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action

Detalhes bibliográficos
Autor(a) principal: Perazzo, Juliana
Data de Publicação: 2017
Outros Autores: Lima, Carla, Heras, Montserrat, Bardají, Eduard, Ferreira, Mônica Lopes, Castanho, Miguel A. R. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/32603
Resumo: © 2017 American Chemical Society
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spelling Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory actionKyotorphinKyotorphin-amideIntravital microscopyGlucocorticoidsMechanism of actionAnti-inflammatory© 2017 American Chemical SocietyNeuropeptide kyotorphin (KTP) is a potent analgesic if administered directly into the brain. In contrast, KTP-amide (KTP-NH2) is analgesic, neuroprotective, and anti-inflammatory following systemic administration, albeit its mechanism of action is unknown. The aim of this study was to shed light on the mechanism of action of KTP-NH2 at the molecular level. KTP-NH2 does not inhibit the enkephalinases angiotensin-converting-enzyme and dipeptidyl-peptidase 3. Intravital microscopy showed that KTP-NH2 decreased the number of rolling leukocytes in a mouse model of inflammation induced by lipopolysaccharide (LPS). Pretreatment with metyrapone abrogated the action of KTP-NH2. Interestingly, stimulating rolling leukocytes using CXCL-1 is also counteracted by the KTP-NH2, but this effect is not abrogated by metyrapone. We conclude that KTP-NH2 has dual action: a glucocorticoid-mediated action, which is dominant in the full-fledged LPS-induced inflammation model, and a glucocorticoid-independent mechanism, which is predominant in models in which leukocyte rolling is stimulated but inflammation is not totally developedFCT-MCTES is acknowledged for Ph.D. fellowship SFRH/BD/52225/2013 to J.P. Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) is acknowledged for funding: call H2020-MSCA-RISE-2014, Grant Agreement 644167, 2015-2019American Chemical SocietyRepositório da Universidade de LisboaPerazzo, JulianaLima, CarlaHeras, MontserratBardají, EduardFerreira, Mônica LopesCastanho, Miguel A. R. B.2018-04-05T10:44:38Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10451/32603engACS Chem. Neurosci. 2017, 8, 1663−16671948-719310.1021/acschemneuro.7b00007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:41:43Zoai:repositorio.ul.pt:10451/32603Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:41:43Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
title Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
spellingShingle Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
Perazzo, Juliana
Kyotorphin
Kyotorphin-amide
Intravital microscopy
Glucocorticoids
Mechanism of action
Anti-inflammatory
title_short Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
title_full Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
title_fullStr Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
title_full_unstemmed Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
title_sort Neuropeptide kyotorphin impacts on lipopolysaccharide-induced glucocorticoid-mediated inflammatory response : a molecular link to nociception, neuroprotection, and anti-inflammatory action
author Perazzo, Juliana
author_facet Perazzo, Juliana
Lima, Carla
Heras, Montserrat
Bardají, Eduard
Ferreira, Mônica Lopes
Castanho, Miguel A. R. B.
author_role author
author2 Lima, Carla
Heras, Montserrat
Bardají, Eduard
Ferreira, Mônica Lopes
Castanho, Miguel A. R. B.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Perazzo, Juliana
Lima, Carla
Heras, Montserrat
Bardají, Eduard
Ferreira, Mônica Lopes
Castanho, Miguel A. R. B.
dc.subject.por.fl_str_mv Kyotorphin
Kyotorphin-amide
Intravital microscopy
Glucocorticoids
Mechanism of action
Anti-inflammatory
topic Kyotorphin
Kyotorphin-amide
Intravital microscopy
Glucocorticoids
Mechanism of action
Anti-inflammatory
description © 2017 American Chemical Society
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-04-05T10:44:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/32603
url http://hdl.handle.net/10451/32603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ACS Chem. Neurosci. 2017, 8, 1663−1667
1948-7193
10.1021/acschemneuro.7b00007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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