Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®

Detalhes bibliográficos
Autor(a) principal: de Miranda, Silvana Spindola
Data de Publicação: 2020
Outros Autores: de Almeida, Isabela Neves, de Fátima Filardi Oliveira Mansur, Maria, de Assis Figueredo, Lida Jouca, da Silva Carvalho, Wânia, Hadaad, João Paulo Amaral, de Lourdes do Carmo Guimarães Diniz, Jaciara, von Groll, Andrea, da Silva, Pedro Almeida, Lopes, Maria Luiza, dos Santos, Marcelo Cordeiro, Brito, Alexandra, de Queiroz Mello, Fernanda Carvalho, da Silva Santos Malaquias, Thiago, Croda, Julio, Pinhata, Juliana Maira Watanabe, de Oliveira, Rosângela Siqueira, Chimara, Erica, Rossetti, Maria Lúcia, Halon, Maria Laura, Lourenço, Maria Cristina, de Melo Medeiros, Reginalda Ferreira, Montes, Fátima Cristina Onofre Fandinho, Machado, Diana, Viveiros, Miguel, Kritski, Afrânio Lineu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116609
Resumo: (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
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spelling Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®a multicenter studyMDRNitrate reductaseTechnologyTuberculosisBiochemistry, Genetics and Molecular Biology (miscellaneous)Infectious DiseasesMicrobiologySDG 3 - Good Health and Well-being(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)RUNde Miranda, Silvana Spindolade Almeida, Isabela Nevesde Fátima Filardi Oliveira Mansur, Mariade Assis Figueredo, Lida Joucada Silva Carvalho, WâniaHadaad, João Paulo Amaralde Lourdes do Carmo Guimarães Diniz, Jaciaravon Groll, Andreada Silva, Pedro AlmeidaLopes, Maria Luizados Santos, Marcelo CordeiroBrito, Alexandrade Queiroz Mello, Fernanda Carvalhoda Silva Santos Malaquias, ThiagoCroda, JulioPinhata, Juliana Maira Watanabede Oliveira, Rosângela SiqueiraChimara, EricaRossetti, Maria LúciaHalon, Maria LauraLourenço, Maria Cristinade Melo Medeiros, Reginalda FerreiraMontes, Fátima Cristina Onofre FandinhoMachado, DianaViveiros, MiguelKritski, Afrânio Lineu2021-05-01T22:51:13Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/116609eng1516-8913PURE: 19727060https://doi.org/10.1590/1678-4324-2020190179info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:16Zoai:run.unl.pt:10362/116609Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:09.793482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
a multicenter study
title Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
spellingShingle Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
de Miranda, Silvana Spindola
MDR
Nitrate reductase
Technology
Tuberculosis
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Microbiology
SDG 3 - Good Health and Well-being
title_short Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
title_full Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
title_fullStr Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
title_full_unstemmed Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
title_sort Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
author de Miranda, Silvana Spindola
author_facet de Miranda, Silvana Spindola
de Almeida, Isabela Neves
de Fátima Filardi Oliveira Mansur, Maria
de Assis Figueredo, Lida Jouca
da Silva Carvalho, Wânia
Hadaad, João Paulo Amaral
de Lourdes do Carmo Guimarães Diniz, Jaciara
von Groll, Andrea
da Silva, Pedro Almeida
Lopes, Maria Luiza
dos Santos, Marcelo Cordeiro
Brito, Alexandra
de Queiroz Mello, Fernanda Carvalho
da Silva Santos Malaquias, Thiago
Croda, Julio
Pinhata, Juliana Maira Watanabe
de Oliveira, Rosângela Siqueira
Chimara, Erica
Rossetti, Maria Lúcia
Halon, Maria Laura
Lourenço, Maria Cristina
de Melo Medeiros, Reginalda Ferreira
Montes, Fátima Cristina Onofre Fandinho
Machado, Diana
Viveiros, Miguel
Kritski, Afrânio Lineu
author_role author
author2 de Almeida, Isabela Neves
de Fátima Filardi Oliveira Mansur, Maria
de Assis Figueredo, Lida Jouca
da Silva Carvalho, Wânia
Hadaad, João Paulo Amaral
de Lourdes do Carmo Guimarães Diniz, Jaciara
von Groll, Andrea
da Silva, Pedro Almeida
Lopes, Maria Luiza
dos Santos, Marcelo Cordeiro
Brito, Alexandra
de Queiroz Mello, Fernanda Carvalho
da Silva Santos Malaquias, Thiago
Croda, Julio
Pinhata, Juliana Maira Watanabe
de Oliveira, Rosângela Siqueira
Chimara, Erica
Rossetti, Maria Lúcia
Halon, Maria Laura
Lourenço, Maria Cristina
de Melo Medeiros, Reginalda Ferreira
Montes, Fátima Cristina Onofre Fandinho
Machado, Diana
Viveiros, Miguel
Kritski, Afrânio Lineu
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
TB, HIV and opportunistic diseases and pathogens (THOP)
RUN
dc.contributor.author.fl_str_mv de Miranda, Silvana Spindola
de Almeida, Isabela Neves
de Fátima Filardi Oliveira Mansur, Maria
de Assis Figueredo, Lida Jouca
da Silva Carvalho, Wânia
Hadaad, João Paulo Amaral
de Lourdes do Carmo Guimarães Diniz, Jaciara
von Groll, Andrea
da Silva, Pedro Almeida
Lopes, Maria Luiza
dos Santos, Marcelo Cordeiro
Brito, Alexandra
de Queiroz Mello, Fernanda Carvalho
da Silva Santos Malaquias, Thiago
Croda, Julio
Pinhata, Juliana Maira Watanabe
de Oliveira, Rosângela Siqueira
Chimara, Erica
Rossetti, Maria Lúcia
Halon, Maria Laura
Lourenço, Maria Cristina
de Melo Medeiros, Reginalda Ferreira
Montes, Fátima Cristina Onofre Fandinho
Machado, Diana
Viveiros, Miguel
Kritski, Afrânio Lineu
dc.subject.por.fl_str_mv MDR
Nitrate reductase
Technology
Tuberculosis
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Microbiology
SDG 3 - Good Health and Well-being
topic MDR
Nitrate reductase
Technology
Tuberculosis
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Microbiology
SDG 3 - Good Health and Well-being
description (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-05-01T22:51:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116609
url http://hdl.handle.net/10362/116609
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1516-8913
PURE: 19727060
https://doi.org/10.1590/1678-4324-2020190179
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eu_rights_str_mv openAccess
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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