Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116609 |
Resumo: | (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. |
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Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®a multicenter studyMDRNitrate reductaseTechnologyTuberculosisBiochemistry, Genetics and Molecular Biology (miscellaneous)Infectious DiseasesMicrobiologySDG 3 - Good Health and Well-being(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)RUNde Miranda, Silvana Spindolade Almeida, Isabela Nevesde Fátima Filardi Oliveira Mansur, Mariade Assis Figueredo, Lida Joucada Silva Carvalho, WâniaHadaad, João Paulo Amaralde Lourdes do Carmo Guimarães Diniz, Jaciaravon Groll, Andreada Silva, Pedro AlmeidaLopes, Maria Luizados Santos, Marcelo CordeiroBrito, Alexandrade Queiroz Mello, Fernanda Carvalhoda Silva Santos Malaquias, ThiagoCroda, JulioPinhata, Juliana Maira Watanabede Oliveira, Rosângela SiqueiraChimara, EricaRossetti, Maria LúciaHalon, Maria LauraLourenço, Maria Cristinade Melo Medeiros, Reginalda FerreiraMontes, Fátima Cristina Onofre FandinhoMachado, DianaViveiros, MiguelKritski, Afrânio Lineu2021-05-01T22:51:13Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/116609eng1516-8913PURE: 19727060https://doi.org/10.1590/1678-4324-2020190179info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:16Zoai:run.unl.pt:10362/116609Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:09.793482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® a multicenter study |
title |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
spellingShingle |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® de Miranda, Silvana Spindola MDR Nitrate reductase Technology Tuberculosis Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Microbiology SDG 3 - Good Health and Well-being |
title_short |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
title_full |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
title_fullStr |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
title_full_unstemmed |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
title_sort |
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase® |
author |
de Miranda, Silvana Spindola |
author_facet |
de Miranda, Silvana Spindola de Almeida, Isabela Neves de Fátima Filardi Oliveira Mansur, Maria de Assis Figueredo, Lida Jouca da Silva Carvalho, Wânia Hadaad, João Paulo Amaral de Lourdes do Carmo Guimarães Diniz, Jaciara von Groll, Andrea da Silva, Pedro Almeida Lopes, Maria Luiza dos Santos, Marcelo Cordeiro Brito, Alexandra de Queiroz Mello, Fernanda Carvalho da Silva Santos Malaquias, Thiago Croda, Julio Pinhata, Juliana Maira Watanabe de Oliveira, Rosângela Siqueira Chimara, Erica Rossetti, Maria Lúcia Halon, Maria Laura Lourenço, Maria Cristina de Melo Medeiros, Reginalda Ferreira Montes, Fátima Cristina Onofre Fandinho Machado, Diana Viveiros, Miguel Kritski, Afrânio Lineu |
author_role |
author |
author2 |
de Almeida, Isabela Neves de Fátima Filardi Oliveira Mansur, Maria de Assis Figueredo, Lida Jouca da Silva Carvalho, Wânia Hadaad, João Paulo Amaral de Lourdes do Carmo Guimarães Diniz, Jaciara von Groll, Andrea da Silva, Pedro Almeida Lopes, Maria Luiza dos Santos, Marcelo Cordeiro Brito, Alexandra de Queiroz Mello, Fernanda Carvalho da Silva Santos Malaquias, Thiago Croda, Julio Pinhata, Juliana Maira Watanabe de Oliveira, Rosângela Siqueira Chimara, Erica Rossetti, Maria Lúcia Halon, Maria Laura Lourenço, Maria Cristina de Melo Medeiros, Reginalda Ferreira Montes, Fátima Cristina Onofre Fandinho Machado, Diana Viveiros, Miguel Kritski, Afrânio Lineu |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) RUN |
dc.contributor.author.fl_str_mv |
de Miranda, Silvana Spindola de Almeida, Isabela Neves de Fátima Filardi Oliveira Mansur, Maria de Assis Figueredo, Lida Jouca da Silva Carvalho, Wânia Hadaad, João Paulo Amaral de Lourdes do Carmo Guimarães Diniz, Jaciara von Groll, Andrea da Silva, Pedro Almeida Lopes, Maria Luiza dos Santos, Marcelo Cordeiro Brito, Alexandra de Queiroz Mello, Fernanda Carvalho da Silva Santos Malaquias, Thiago Croda, Julio Pinhata, Juliana Maira Watanabe de Oliveira, Rosângela Siqueira Chimara, Erica Rossetti, Maria Lúcia Halon, Maria Laura Lourenço, Maria Cristina de Melo Medeiros, Reginalda Ferreira Montes, Fátima Cristina Onofre Fandinho Machado, Diana Viveiros, Miguel Kritski, Afrânio Lineu |
dc.subject.por.fl_str_mv |
MDR Nitrate reductase Technology Tuberculosis Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Microbiology SDG 3 - Good Health and Well-being |
topic |
MDR Nitrate reductase Technology Tuberculosis Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Microbiology SDG 3 - Good Health and Well-being |
description |
(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-05-01T22:51:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116609 |
url |
http://hdl.handle.net/10362/116609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1516-8913 PURE: 19727060 https://doi.org/10.1590/1678-4324-2020190179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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9 application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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