Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8390 |
Resumo: | This article belongs to the Special Issue Emerging Themes in Brucella and Brucellosis. |
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Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the MethodBrucella MelitensisAntimicrobial Susceptibility TestingInterlaboratory ValidationISO 20776 standardCLSIInfecções Sistémicas e ZoonosesThis article belongs to the Special Issue Emerging Themes in Brucella and Brucellosis.Brucellosis, mainly caused by Brucella (B.) melitensis, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for B. melitensis are not available, and the agent is not yet listed in the EUCAST breakpoint tables. CLSI recommendations for B. melitensis exist, but they do not fulfill the requirements of the ISO 20776 standard regarding the culture medium and the incubation conditions. Under the third EU Health Programme, laboratories specializing in the diagnostics of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aiming to develop a suitable method for the AST of B. melitensis. Under the supervision of EUCAST representatives, this working group adapted the CLSI M45 document to the ISO 20776 standard after testing and validation. These adaptations included the comparison of various culture media, culture conditions and AST methods. A Standard Operation Procedure was derived and an interlaboratory validation was performed in order to evaluate the method. The results showed pros and cons for both of the two methods but also indicate that it is not necessary to abandon Mueller–Hinton without additives for the AST of B. melitensis.This research was funded by the EU Health Programme 2014–2020, through the Consumers, Health, Agriculture and Food Executive Agency (CHAFEA, European Commission), the Joint Action EMERGE (CHAFEA n° 677 066) and the Joint Action SHARP (848096-SHARP JA).MDPIRepositório Científico do Instituto Nacional de SaúdeTscherne, A.Mantel, E.Boskani, T.Budniak, S.Elschner, M.Fasanella, A.Feruglio, S.L.Galante, D.Giske, C.G.Grunow, RolandHenczko, JuditHinz, ChristinIwaniak, WojciechJacob, DanielaKedrak-Jablonska, AgnieszkaJensen, Veronica K.Johansen, Tone B.Kahlmeter, GunnarManzulli, VivianaMatuschek, ErikaMelzer, FalkNuncio, Maria S.Papaparaskevas, JosephPelerito, AnaSolheim, MargreteThomann, SusanneTsakris, AthanasiosWahab, TaraWeiner, MarcinZoeller, LotharZange, SabineEMERGE AST Working Group2022-12-09T15:24:26Z2022-07-202022-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8390engMicroorganisms. 2022 Jul 20;10(7):1470. doi: 10.3390/microorganisms100714702076-260710.3390/microorganisms10071470info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:29Zoai:repositorio.insa.pt:10400.18/8390Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:57.320628Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| spellingShingle |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method Tscherne, A. Brucella Melitensis Antimicrobial Susceptibility Testing Interlaboratory Validation ISO 20776 standard CLSI Infecções Sistémicas e Zoonoses |
| title_short |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_full |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_fullStr |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_full_unstemmed |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| title_sort |
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method |
| author |
Tscherne, A. |
| author_facet |
Tscherne, A. Mantel, E. Boskani, T. Budniak, S. Elschner, M. Fasanella, A. Feruglio, S.L. Galante, D. Giske, C.G. Grunow, Roland Henczko, Judit Hinz, Christin Iwaniak, Wojciech Jacob, Daniela Kedrak-Jablonska, Agnieszka Jensen, Veronica K. Johansen, Tone B. Kahlmeter, Gunnar Manzulli, Viviana Matuschek, Erika Melzer, Falk Nuncio, Maria S. Papaparaskevas, Joseph Pelerito, Ana Solheim, Margrete Thomann, Susanne Tsakris, Athanasios Wahab, Tara Weiner, Marcin Zoeller, Lothar Zange, Sabine EMERGE AST Working Group |
| author_role |
author |
| author2 |
Mantel, E. Boskani, T. Budniak, S. Elschner, M. Fasanella, A. Feruglio, S.L. Galante, D. Giske, C.G. Grunow, Roland Henczko, Judit Hinz, Christin Iwaniak, Wojciech Jacob, Daniela Kedrak-Jablonska, Agnieszka Jensen, Veronica K. Johansen, Tone B. Kahlmeter, Gunnar Manzulli, Viviana Matuschek, Erika Melzer, Falk Nuncio, Maria S. Papaparaskevas, Joseph Pelerito, Ana Solheim, Margrete Thomann, Susanne Tsakris, Athanasios Wahab, Tara Weiner, Marcin Zoeller, Lothar Zange, Sabine EMERGE AST Working Group |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Tscherne, A. Mantel, E. Boskani, T. Budniak, S. Elschner, M. Fasanella, A. Feruglio, S.L. Galante, D. Giske, C.G. Grunow, Roland Henczko, Judit Hinz, Christin Iwaniak, Wojciech Jacob, Daniela Kedrak-Jablonska, Agnieszka Jensen, Veronica K. Johansen, Tone B. Kahlmeter, Gunnar Manzulli, Viviana Matuschek, Erika Melzer, Falk Nuncio, Maria S. Papaparaskevas, Joseph Pelerito, Ana Solheim, Margrete Thomann, Susanne Tsakris, Athanasios Wahab, Tara Weiner, Marcin Zoeller, Lothar Zange, Sabine EMERGE AST Working Group |
| dc.subject.por.fl_str_mv |
Brucella Melitensis Antimicrobial Susceptibility Testing Interlaboratory Validation ISO 20776 standard CLSI Infecções Sistémicas e Zoonoses |
| topic |
Brucella Melitensis Antimicrobial Susceptibility Testing Interlaboratory Validation ISO 20776 standard CLSI Infecções Sistémicas e Zoonoses |
| description |
This article belongs to the Special Issue Emerging Themes in Brucella and Brucellosis. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-12-09T15:24:26Z 2022-07-20 2022-07-20T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.18/8390 |
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http://hdl.handle.net/10400.18/8390 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Microorganisms. 2022 Jul 20;10(7):1470. doi: 10.3390/microorganisms10071470 2076-2607 10.3390/microorganisms10071470 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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