Evaluation of zearalenone in flours

Detalhes bibliográficos
Autor(a) principal: Ramos Aldana, Juan
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/26089
Resumo: Dissertação de mestrado em Segurança Alimentar, apresentada à Faculdade de Farmácia da Universidade de Coimbra.
id RCAP_32c12b01412f3dba980aa2682a7ce4de
oai_identifier_str oai:estudogeral.uc.pt:10316/26089
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evaluation of zearalenone in floursFarinhaContaminação alimentarZearalenonaDissertação de mestrado em Segurança Alimentar, apresentada à Faculdade de Farmácia da Universidade de Coimbra.An analytical methodology based on extraction with acetonitrile:water (90:10), clean-up with immunoaffinity columns (IACs), and detection and quantification by liquid chromatography with fluorescence detection (LC-FD) was validated in order to evaluate zearalenone (ZEA) in different types of flours (wheat, maize, mixed cereals) used for human consumption with different purposes, originated from Coimbra (Portugal), Utrecht (The Netherlands) and Valencia (Spain). Linearity, in the working standards solutions, between 12.5 ng/mL and 200ng/mL, was good (r2=0.998). Linearity in the matrix-matched assay, prepared between 20 and 250μg/Kg, was r2=0.997. Matrix-effect was 92.5%. Recovery values ranged between 97.6 and 105.3%, and precision between 2 and 13.6%. The accuracy and precision results comply with the requirements established by the EC directive 401/2006. LOD and LOQ were 3.75 and 12.5μg/Kg, respectively. The application of the procedure to 50 samples from the three cities showed that 36% of the samples were contaminated. One sample with baby flour purpose exceeded the maximum limit established by EC legislation of 2007, and another one was close to the limit. A maize flour sample exceeded the ML established by EC with a concentration of 111.7μg/kg. Thus, two of the tested samples from Coimbra were contaminated above the established maximum limits for processed maize-based food for infants and maize intended for direct human consumption. The estimated daily intake (EDI) ranged between 0.013 and 0.14 g/kg b.w./day, which represents 52x102% and 560x102% of the TDI established by EFSA in 2011, 0.25 ng/kg b.w./day. Therefore, all the studied populations are at risk, being this risk higher for babies than for adults, both in Portuguese and Dutch population.2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://hdl.handle.net/10316/26089http://hdl.handle.net/10316/26089porRamos Aldana, Juaninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-01-20T17:48:40Zoai:estudogeral.uc.pt:10316/26089Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:46.377986Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of zearalenone in flours
title Evaluation of zearalenone in flours
spellingShingle Evaluation of zearalenone in flours
Ramos Aldana, Juan
Farinha
Contaminação alimentar
Zearalenona
title_short Evaluation of zearalenone in flours
title_full Evaluation of zearalenone in flours
title_fullStr Evaluation of zearalenone in flours
title_full_unstemmed Evaluation of zearalenone in flours
title_sort Evaluation of zearalenone in flours
author Ramos Aldana, Juan
author_facet Ramos Aldana, Juan
author_role author
dc.contributor.author.fl_str_mv Ramos Aldana, Juan
dc.subject.por.fl_str_mv Farinha
Contaminação alimentar
Zearalenona
topic Farinha
Contaminação alimentar
Zearalenona
description Dissertação de mestrado em Segurança Alimentar, apresentada à Faculdade de Farmácia da Universidade de Coimbra.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/26089
http://hdl.handle.net/10316/26089
url http://hdl.handle.net/10316/26089
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133756078948352

Registros relacionados