hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation

Detalhes bibliográficos
Autor(a) principal: Miranda, Cláudia C.
Data de Publicação: 2020
Outros Autores: Barata, Tiago, Vaz, Sandra H., Ferreira, Carla, Quintas, Alexandre, Bekman, Evguenia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/53284
Resumo: Copyright © 2020 Miranda, Barata, Vaz, Ferreira, Quintas and Bekman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
id RCAP_3324bb7d1598e7b99270e5f98d3dc270
oai_identifier_str oai:repositorio.ul.pt:10451/53284
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiationCBDEG-018THJ-018hiPSCNeuronal differentiationPhytocannabinoidsSynthetic cannabinoidsΔ9-THCCopyright © 2020 Miranda, Barata, Vaz, Ferreira, Quintas and Bekman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Phytocannabinoids are psychotropic substances ofcannabis with the ability to bind endocannabinoid (eCB) receptors that regulate synaptic activity in the central nervous system (CNS). Synthetic cannabinoids (SCs) are synthetic analogs of Δ9-tetrahydrocannabinol (Δ9-THC), the psychotropic compound of cannabis, acting as agonists of eCB receptor CB1. SC is an easily available and popular alternative to cannabis, and their molecular structure is always changing, increasing the hazard for the general population. The popularity of cannabis and its derivatives may lead, and often does, to a child's exposure to cannabis both in utero and through breastfeeding by a drug-consuming mother. Prenatal exposure to cannabis has been associated with an altered rate of mental development and significant changes in nervous system functioning. However, the understanding of mechanisms of its action on developing the human CNS is still lacking. We investigated the effect of continuous exposure to cannabinoids on developing human neurons, mimicking the prenatal exposure by drug-consuming mother. Two human induced pluripotent stem cells (hiPSC) lines were induced to differentiate into neuronal cells and exposed for 37 days to cannabidiol (CBD), Δ9-THC, and two SCs, THJ-018 and EG-018. Both Δ9-THC and SC, at 10 μM, promote precocious neuronal and glial differentiation, while CBD at the same concentration is neurotoxic. Neurons exposed to Δ9-THC and SC show abnormal functioning of voltage-gated calcium channels when stimulated by extracellular potassium. In sum, all studied substances have a profound impact on the developing neurons, highlighting the importance of thorough research on the impact of prenatal exposure to natural and SC.This work was supported by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (SFRH/BPD/81627/2011 to SV), by iBB — Institute for Bioengineering and Biosciences — project UIDB/04565/2020, and by Egas Moniz Higher Institute of Health Science (Egas Moniz, CRL). Funding was also received from the European Union’s Horizon 2020 Research and Innovation programme, under the Grant Agreement number 739572—The Discoveries Centre for Regenerative and Precision Medicine H2020-WIDESPREAD-01-2016-2017 to EB.FrontiersRepositório da Universidade de LisboaMiranda, Cláudia C.Barata, TiagoVaz, Sandra H.Ferreira, CarlaQuintas, AlexandreBekman, Evguenia2022-06-06T14:41:39Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53284engFront Mol Neurosci. 2020 Jul 6;13:11910.3389/fnmol.2020.001191662-5099info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:58:59Zoai:repositorio.ul.pt:10451/53284Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:15.723025Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
title hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
spellingShingle hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
Miranda, Cláudia C.
CBD
EG-018
THJ-018
hiPSC
Neuronal differentiation
Phytocannabinoids
Synthetic cannabinoids
Δ9-THC
title_short hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
title_full hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
title_fullStr hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
title_full_unstemmed hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
title_sort hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal differentiation
author Miranda, Cláudia C.
author_facet Miranda, Cláudia C.
Barata, Tiago
Vaz, Sandra H.
Ferreira, Carla
Quintas, Alexandre
Bekman, Evguenia
author_role author
author2 Barata, Tiago
Vaz, Sandra H.
Ferreira, Carla
Quintas, Alexandre
Bekman, Evguenia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Miranda, Cláudia C.
Barata, Tiago
Vaz, Sandra H.
Ferreira, Carla
Quintas, Alexandre
Bekman, Evguenia
dc.subject.por.fl_str_mv CBD
EG-018
THJ-018
hiPSC
Neuronal differentiation
Phytocannabinoids
Synthetic cannabinoids
Δ9-THC
topic CBD
EG-018
THJ-018
hiPSC
Neuronal differentiation
Phytocannabinoids
Synthetic cannabinoids
Δ9-THC
description Copyright © 2020 Miranda, Barata, Vaz, Ferreira, Quintas and Bekman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2022-06-06T14:41:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/53284
url http://hdl.handle.net/10451/53284
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front Mol Neurosci. 2020 Jul 6;13:119
10.3389/fnmol.2020.00119
1662-5099
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134593118371840

Registros relacionados