New insights into resistance to colistin and third-generation cephalosporins of Escherichia coli in poultry, Portugal: Novel blaCTX-M-166 and blaESAC genes

• Autor(a) principal: Manageiro, Vera
• Data de Publicação: 2017
• Outros Autores: Clemente, Lurdes, Graça, Rafael, Correia, Ivone, Albuquerque, Teresa, Ferreira, Eugénia, Caniça, Manuela
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.18/5352
• topic: Animals; Anti-Bacterial Agents; Cephalosporins; Chickens; Colistin; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Food Contamination; Plasmids; Portugal; Poultry Diseases; Turkeys; beta-Lactamases; Resistência aos Antimicrobianos

The increasing incidence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and Gram negative organisms that has been observed in food animals such as poultry, cattle and pigs, are suggestive that animals, food and environment are potential sources of ESBL-producing bacteria. Hence, the aim of this study was to characterized commensal E. coli obtained from healthy broiler and turkey flocks at slaughter for the presence of penicillinases-, ESBL-, extended-spectrum AmpC (ESAC)-, plasmid-mediated quinolone resistance- and MCR-encoding genes. Study of clonal relatedness showed genetic diversity among CTX-M-type, SHV-12 and TEM-52 producing isolates with human isolates of the same type, was also assessed. We detected that eleven (5.4%, 11/202) and forty-five (2.2%, 45/185) E. coli isolates from broilers and turkeys, respectively, carried blaESBLor blaESACgenes and two isolates from turkeys carried mcr-1 gene. A new variant blaCTX-M-166was reported in a multidrug resistant isolate from a broiler flock. Overall, we detected a diversity of resistance mechanisms among E. coli from food-producing animals, all of them with high importance at a public health level.